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@article{Shimolina:2020:10.1117/1.JBO.25.12.126004,
author = {Shimolina, LE and Gulin, AA and Paez-Perez, M and Lopez-Duarte, I and Druzhkova, IN and Lukina, MM and Gubina, M and Brooks, NJ and Zagaynova, E and Kuimova, MK and Shirmanova, M},
doi = {10.1117/1.JBO.25.12.126004},
journal = {Journal of Biomedical Optics},
pages = {1--16},
title = {Mapping cisplatin-induced viscosity alterations in cancer cells using molecular rotor and fluorescence lifetime imaging microscopy},
url = {http://dx.doi.org/10.1117/1.JBO.25.12.126004},
volume = {25},
year = {2020}
}

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TY  - JOUR
AB  - Significance: Despite the importance of the cell membrane in regulation of drug activity, the influence of drug treatments on its physical properties is still poorly understood. The combination of fluorescence lifetime imaging microscopy (FLIM) with specific viscosity-sensitive fluorescent molecular rotors allows the quantification of membrane viscosity with high spatiotemporal resolution, down to the individual cell organelles.Aim: The aim of our work was to analyze microviscosity of the plasma membrane of living cancer cells during chemotherapy with cisplatin using FLIM and correlate the observed changes with lipid composition and cell’s response to treatment.Approach: FLIM together with viscosity-sensitive boron dipyrromethene-based fluorescent molecular rotor was used to map the fluidity of the cell’s membrane. Chemical analysis of membrane lipid composition was performed with time-of-flight secondary ion mass spectrometry (ToF-SIMS).Results: We detected a significant steady increase in membrane viscosity in viable cancer cells, both in cell monolayers and tumor spheroids, upon prolonged treatment with cisplatin, as well as in cisplatin-adapted cell line. ToF-SIMS revealed correlative changes in lipid profile of cisplatin-treated cells.Conclusions: These results suggest an involvement of membrane viscosity in the cell adaptation to the drug and in the acquisition of drug resistance.
AU  - Shimolina,LE
AU  - Gulin,AA
AU  - Paez-Perez,M
AU  - Lopez-Duarte,I
AU  - Druzhkova,IN
AU  - Lukina,MM
AU  - Gubina,M
AU  - Brooks,NJ
AU  - Zagaynova,E
AU  - Kuimova,MK
AU  - Shirmanova,M
DO  - 10.1117/1.JBO.25.12.126004
EP  - 16
PY  - 2020///
SN  - 1083-3668
SP  - 1
TI  - Mapping cisplatin-induced viscosity alterations in cancer cells using molecular rotor and fluorescence lifetime imaging microscopy
T2  - Journal of Biomedical Optics
UR  - http://dx.doi.org/10.1117/1.JBO.25.12.126004
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000605144900013&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.spiedigitallibrary.org/journals/journal-of-biomedical-optics/volume-25/issue-12/126004/Mapping-cisplatin-induced-viscosity-alterations-in-cancer-cells-using-molecular/10.1117/1.JBO.25.12.126004.full?SSO=1
UR  - http://hdl.handle.net/10044/1/87216
VL  - 25
ER  -

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