Publications
183 results found
Khurshid S, Saridakis E, Govada L, et al., 2014, Porous nucleating agents for protein crystallization, Nature Protocols, Vol: 9, Pages: 1621-1633, ISSN: 1750-2799
Solving the structure of proteins is pivotal to achieving success in rational drug design and in other biotechnological endeavors. The most powerful method for determining the structure of proteins is X-ray crystallography, which relies on the availability of high-quality crystals. However, obtaining such crystals is a major hurdle. Nucleation is the crucial prerequisite step, which requires overcoming an energy barrier. The presence in a protein solution of a nucleant, a solid or a semiliquid substance that facilitates overcoming that barrier allows crystals to grow under ideal conditions, paving the way for the formation of high-quality crystals. The use of nucleants provides a unique means for optimizing the diffraction quality of crystals, as well as for discovering new crystallization conditions. We present a protocol for controlling the nucleation of protein crystals that is applicable to a wide variety of nucleation-inducing substances. Setting up crystallization trials using these nucleating agents takes an additional few seconds compared with conventional setup, and it can accelerate crystallization, which typically takes several days to months.
Lansky S, Alalouf O, Solomon V, et al., 2014, Crystallization and preliminary crystallographic analysis of Axe2, an acetylxylan esterase from <i>Geobacillus stearothermophilus</i>. (vol 69, pg 430, 2013), ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, Vol: 70, Pages: 685-685
Chayen NE, Shaffer M, Leese H, et al., 2014, NUCLEANT FOR MACROMOLECULE CRYSTALLISATION.
Lansky S, Alalouf O, Solomon HV, et al., 2014, A unique octameric structure of Axe2, an intracellular acetyl-xylooligosaccharide esterase from <i>Geobacillus stearothermophilus</i>, ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, Vol: 70, Pages: 261-278, ISSN: 1399-0047
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- Citations: 26
Govada L, Saridakis E, Khurshid S, et al., 2014, Enhancing the success of crystallization: strategies and techniques, Publisher: INT UNION CRYSTALLOGRAPHY, Pages: C1082-C1082, ISSN: 2053-2733
Govada L, Saridakis E, Kassen S, et al., 2014, Smart materials for increasing the success of protein crystallization, Publisher: INT UNION CRYSTALLOGRAPHY, Pages: C1138-C1138, ISSN: 2053-2733
Solomon HV, Tabachnikov O, Feinberg H, et al., 2013, Crystallization and preliminary crystallographic analysis of GanB, a GH42 intracellular β-galactosidase from <i>Geobacillus stearothermophilus</i>, ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, Vol: 69, Pages: 1114-1119
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- Citations: 19
Saridakis E, Chayen NE, 2013, Imprinted polymers assisting protein crystallization, TRENDS IN BIOTECHNOLOGY, Vol: 31, Pages: 515-520, ISSN: 0167-7799
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- Citations: 41
Lansky S, Alalouf O, Solomon V, et al., 2013, Crystallization and preliminary crystallographic analysis of Axe2, an acetylxylan esterase from <i>Geobacillus stearothermophilus</i>, ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, Vol: 69, Pages: 430-434
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- Citations: 17
Kertis F, Khurshid S, Okman O, et al., 2012, Heterogeneous nucleation of protein crystals using nanoporous gold nucleants, JOURNAL OF MATERIALS CHEMISTRY, Vol: 22, Pages: 21928-21934, ISSN: 0959-9428
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- Citations: 45
Glassford SE, Govada L, Chayen NE, et al., 2012, Micro ATR FTIR imaging of hanging drop protein crystallisation, VIBRATIONAL SPECTROSCOPY, Vol: 63, Pages: 492-498, ISSN: 0924-2031
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- Citations: 20
Reddy SM, Phan QT, El-Sharif H, et al., 2012, Protein Crystallization and Biosensor Applications of Hydrogel-based Molecularly Imprinted Polymers (HydroMIPs), Biomacromolecules, Vol: 13, Pages: 3959-3965
Stiegler AL, Draheim KM, Li X, et al., 2012, Structural Basis for Paxillin Binding and Focal Adhesion Targeting of β-Parvin, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 287, Pages: 32566-32577
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- Citations: 29
Mckie AB, Vaughan S, Zanini E, et al., 2012, The OPCML Tumor Suppressor Functions as a Cell Surface Repressor–Adaptor, Negatively Regulating Receptor Tyrosine Kinases in Epithelial Ovarian Cancer, Cancer Discovery
Stewart PDS, Kolek SA, Briggs RA, et al., 2012, Microseed it! A Theoretical and Practical Exploration of Seed Stability and Seeding Techniques for Successful Protein Crystallization, Publisher: INT UNION CRYSTALLOGRAPHY, Pages: S142-S142, ISSN: 2053-2733
Chayen NE, 2012, Smart materials for protein crystallization, Publisher: INT UNION CRYSTALLOGRAPHY, Pages: S11-S11, ISSN: 2053-2733
Saridakis E, Khurshid S, Govada L, et al., 2011, Protein crystallization facilitated by molecularly imprinted polymers (vol 108, pg 11081, 2011), PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 108, Pages: 18566-18566, ISSN: 0027-8424
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- Citations: 3
Boudjemline A, Saridakis E, Swann MJ, et al., 2011, Use of Dual Polarization Interferometry as a Diagnostic Tool for Protein Crystallization, ANALYTICAL CHEMISTRY, Vol: 83, Pages: 7881-7887, ISSN: 0003-2700
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- Citations: 12
Stewart PDS, Kolek SA, Briggs RA, et al., 2011, Random Microseeding: A Theoretical and Practical Exploration of Seed Stability and Seeding Techniques for Successful Protein Crystallization, CRYSTAL GROWTH & DESIGN, Vol: 11, Pages: 3432-3441, ISSN: 1528-7483
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- Citations: 65
Saridakis E, Khurshid S, Govada L, et al., 2011, Protein crystallization facilitated by molecularly imprinted polymers, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 108, Pages: 11081-11086, ISSN: 0027-8424
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- Citations: 106
Abrahams J-P, Apweiler R, Balling R, et al., 2011, "4D Biology for health and disease" workshop report, NEW BIOTECHNOLOGY, Vol: 28, Pages: 291-293, ISSN: 1871-6784
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- Citations: 1
Garcia-Caballero A, Gavira JA, Pineda-Molina E, et al., 2011, Optimization of Protein Crystallization: The OptiCryst Project, CRYSTAL GROWTH & DESIGN, Vol: 11, Pages: 2112-2121, ISSN: 1528-7483
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- Citations: 12
McKie AB, Vaughan S, Okon I, et al., 2011, The OPCML tumor suppressor negatively regulates a specific repertoire of 5 receptor tyrosine kinases via a novel proteasomal mechanism, and its recombinant derivative is a potent in-vivo anticancer protein therapy, CANCER RESEARCH, Vol: 71, ISSN: 0008-5472
Petit SJ, Wise EL, Chambers JC, et al., 2011, The CXCL16 A181V Mutation Selectively Inhibits Monocyte Adhesion to CXCR6 but Is Not Associated With Human Coronary Heart Disease, ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, Vol: 31, Pages: 914-920, ISSN: 1079-5642
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- Citations: 16
Kulkarni MV, Tettamanzi MC, Murphy JW, et al., 2010, Two Independent Histidines, One in Human Prolactin and One in Its Receptor, Are Critical for pH-dependent Receptor Recognition and Activation, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 285, Pages: 38524-38533
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- Citations: 25
Khurshid S, Haire LF, Chayen NE, 2010, Automated seeding for the optimization of crystal quality, JOURNAL OF APPLIED CRYSTALLOGRAPHY, Vol: 43, Pages: 752-756, ISSN: 0021-8898
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- Citations: 12
Kertis F, Snyder J, Govada L, et al., 2010, Structure/Processing Relationships in the Fabrication of Nanoporous Gold, JOM, Vol: 62, Pages: 50-56, ISSN: 1047-4838
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- Citations: 79
Chayen N, Khurshid S, 2010, Upside Down Protein Crystallization., Publisher: INT UNION CRYSTALLOGRAPHY, Pages: S294-S294, ISSN: 2053-2733
Saridakis E, Boudjemline A, Swann MJ, et al., 2010, Dual Polarization Interferometry in Macromolecular Crystallisation Diagnostics., Publisher: INT UNION CRYSTALLOGRAPHY, Pages: S293-S293, ISSN: 2053-2733
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