Imperial College London
Alternate

ProfessorNaomiChayen

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Prof Res Fellow - Professor of Biomedical Sciences
 
 
 
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Contact

 

+44 (0)20 7594 3240n.chayen Website

 
 
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Location

 

Office no. E304Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Li:2019:10.3390/cryst9090462,
author = {Li, Y and Govada, L and Solomon, HV and Gillis, RB and Adams, GG and Chayen, NE},
doi = {10.3390/cryst9090462},
journal = {Crystals},
title = {Analysis of glulisine crystallisation utilising phase diagrams and nucleants},
url = {http://dx.doi.org/10.3390/cryst9090462},
volume = {9},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Glulisine is a US Food and Drug Administration (FDA) approved insulin analogue, used for controlling hyperglycaemia in patients with diabetes mellitus (DM). It is fast acting which better approximates physiological insulin secretion, improving patient outcome. Crystallisation of Glulisine was analysed by its crystallisation phase diagram and nucleation-inducing materials. Both the hanging drop vapour diffusion and microbatch-under-oil methods were used and compared. We have shown that the same protein can have different solubility behaviours depending on the nature of the salt in the precipitating agent. In the case of Glulisine with magnesium formate, lowering the precipitant concentration drove the system further into supersaturation resulting in the formation of crystals and precipitation. This was the opposite effect to the usual scenario where raising the precipitant concentration leads to supersaturation. Glulisine with sodium potassium tartrate tetrahydrate (NaKT) followed the expected trend of forming crystals or precipitate at higher concentrations and clear drops at lower concentrations of the precipitant. The outcomes of crystallisation using the different crystallisation methods is also described. Glulisine was successfully crystallised and the crystals diffracted up to a resolution limit of 1.4 Å.
AU  - Li,Y
AU  - Govada,L
AU  - Solomon,HV
AU  - Gillis,RB
AU  - Adams,GG
AU  - Chayen,NE
DO  - 10.3390/cryst9090462
PY  - 2019///
SN  - 2073-4352
TI  - Analysis of glulisine crystallisation utilising phase diagrams and nucleants
T2  - Crystals
UR  - http://dx.doi.org/10.3390/cryst9090462
UR  - http://hdl.handle.net/10044/1/73129
VL  - 9
ER  -
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