Imperial College London
Alternate

ProfessorNaomiChayen

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Prof Res Fellow - Professor of Biomedical Sciences
 
 
 
//

Contact

 

+44 (0)20 7594 3240n.chayen Website

 
 
//

Location

 

Office no. E304Burlington DanesHammersmith Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Chayen:2016:10.1038/srep20053,
author = {Chayen, N and shaffer and govada and Khurshid and Kassen and Leese, H S and Hu, S and Menzel and Chain, B and Saridakis},
doi = {10.1038/srep20053},
journal = {Scientific Reports},
title = {Exploring Carbon Nanomaterial Diversity for Nucleation of Protein Crystals},
url = {http://dx.doi.org/10.1038/srep20053},
volume = {6},
year = {2016}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Controlling crystal nucleation is a crucial step in obtaining high quality protein crystals for structure determination by X-ray crystallography. Carbon nanomaterials (CNMs) including carbon nanotubes, graphene oxide, and carbon black provide a range of surface topographies, porosities and length scales; functionalisation with two different approaches, gas phase radical grafting and liquid phase reductive grafting, provide routes to a range of oligomer functionalised products. These grafted materials, combined with a range of controls, were used in a large-scale assessment of the effectiveness for protein crystal nucleation of 20 different carbon nanomaterials on five proteins. This study has allowed a direct comparison of the key characteristics of carbon-based nucleants: appropriate surface chemistry, porosity and/or roughness are required. The most effective solid system tested in this study, carbon black nanoparticles functionalised with poly(ethylene glycol) methyl ether of mean molecular weight 5000, provides a novel highly effective nucleant, that was able to induce crystal nucleation of four out of the five proteins tested at metastable conditions.
AU  - Chayen,N
AU  - shaffer
AU  - govada
AU  - Khurshid
AU  - Kassen
AU  - Leese,H S
AU  - Hu,S
AU  - Menzel
AU  - Chain,B
AU  - Saridakis
DO  - 10.1038/srep20053
PY  - 2016///
SN  - 2045-2322
TI  - Exploring Carbon Nanomaterial Diversity for Nucleation of Protein Crystals
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/srep20053
UR  - http://hdl.handle.net/10044/1/28245
VL  - 6
ER  -
Download