Imperial College London

DrNickDibb

Faculty of MedicineDepartment of Metabolism, Digestion and Reproduction

Reader in Cell Signalling
 
 
 
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Contact

 

+44 (0)20 7594 2103n.dibb

 
 
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Location

 

5009Hammersmith HospitalHammersmith Campus

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Summary

 

Publications

Publication Type
Year
to

48 results found

Tan GC, Wong YP, Cui W, Dibb Net al., 2020, Construction of a doxycycline inducible lentivirus that expresses stem cell-specific miR-302 cluster, MALAYSIAN JOURNAL OF PATHOLOGY, Vol: 42, Pages: 91-97, ISSN: 0126-8635

Journal article

Chandrashekran A, Casimir C, Dibb N, Readhead C, Winston Ret al., 2016, Generating Transgenic Mice by Lentiviral Transduction of Spermatozoa Followed by In Vitro Fertilization and Embryo Transfer., Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools., Publisher: Humana Press, Pages: 95-106, ISBN: 978-1-4939-3751-6

Most transgenic technologies rely on the oocyte as a substrate for genetic modification. Transgenics animals are usually generated by the injection of the gene constructs (including lentiviruses encoding gene constructs or modified embryonic stem cells) into the pronucleus of a fertilized egg followed by the transfer of the injected embryos into the uterus of a foster mother. Male germ cells also have potential as templates for transgenic development. We have previously shown that mature sperm can be utilized as template for lentiviral transduction and as such used to generate transgenic mice efficiently with germ line capabilities. We provide here a detailed protocol that is relatively simple, to establish transgenic mice using lentivirally transduced spermatozoa. This protocol employs a well-established lentiviral gene delivery system (usual for somatic cells) delivering a variety of transgenes to be directly used with sperm, and the subsequent use of these modified sperm in in vitro fertilization studies and embryo transfer into foster female mice, for the establishment of transgenic mice.

Book chapter

Tan GC, Dibb N, 2015, IsomiRs have functional importance, MALAYSIAN JOURNAL OF PATHOLOGY, Vol: 37, Pages: 73-81, ISSN: 0126-8635

Journal article

Tan GC, Chan E, Molnar A, Sarkar R, Alexieva D, Isa IM, Robinson S, Zhang S, Ellis P, Langford CF, Guillot PV, Chandrashekran A, Fisk NM, Castellano L, Meister G, Winston RM, Cui W, Baulcombe D, Dibb NJet al., 2014, 5 ' isomiR variation is of functional and evolutionary importance, Nucleic Acids Research, Vol: 42, Pages: 9424-9435, ISSN: 1362-4962

We have sequenced miRNA libraries from human embryonic,neural and foetal mesenchymal stem cells.We report that the majority of miRNA genes encodemature isomers that vary in size by one ormore bases at the 3 and/or 5 end of the miRNA.Northern blotting for individual miRNAs showed thatthe proportions of isomiRs expressed by a singlemiRNA gene often differ between cell and tissuetypes. IsomiRs were readily co-immunoprecipitatedwith Argonaute proteins in vivo and were active inluciferase assays, indicating that they are functional.Bioinformatics analysis predicts substantial differencesin targeting between miRNAs with minor 5differences and in support of this we report that a 5isomiR-9–1 gained the ability to inhibit the expressionof DNMT3B and NCAM2 but lost the ability toinhibit CDH1 in vitro. This result was confirmed bythe use of isomiR-specific sponges. Our analysis ofthe miRGator database indicates that a small percentageof human miRNA genes express isomiRs asthe dominant transcript in certain cell types and analysisof miRBase shows that 5 isomiRs have replacedcanonical miRNAs many times during evolution. Thisstrongly indicates that isomiRs are of functional importanceand have contributed to the evolution ofmiRNA genes.INT

Journal article

Chandrashekran A, Sarkar R, Thrasher A, Fraser SE, Dibb N, Casimir C, Winston R, Readhead Cet al., 2014, Efficient generation of transgenic mice by lentivirus-mediated modification of spermatozoa, FASEB JOURNAL, Vol: 28, Pages: 569-576, ISSN: 0892-6638

Journal article

Chandrashekran A, Isa I, Dudhia J, Thrasher AJ, Dibb N, Casimir C, Readhead C, Winston Ret al., 2014, Lentiviral vector transduction of spermatozoa as a tool for the study of early development, FEBS OPEN BIO, Vol: 4, Pages: 266-275, ISSN: 2211-5463

Journal article

Tan GC, Dibb NJ, 2012, MicroRNA-induced pluripotent stem cells., Malays J Pathol, Vol: 34, Pages: 167-168, ISSN: 0126-8635

Journal article

Kapustin Y, Chan E, Sarkar R, Wong F, Vorechovsky I, Winston RM, Tatusova T, Dibb NJet al., 2011, Cryptic splice sites and split genes, NUCLEIC ACIDS RESEARCH, Vol: 39, Pages: 5837-5844, ISSN: 0305-1048

Journal article

Zhou AY, Ichaso N, Adamarek A, Zila V, Forstova J, Dibb NJ, Dilworth SMet al., 2011, Polyomavirus Middle T-Antigen Is a Transmembrane Protein That Binds Signaling Proteins in Discrete Subcellular Membrane Sites, JOURNAL OF VIROLOGY, Vol: 85, Pages: 3046-3054, ISSN: 0022-538X

Journal article

Lee KC, Ouwehand I, Giannini AL, Thomas NS, Dibb NJ, Bijlmakers MJet al., 2010, Lck is a key target of imatinib and dasatinib in T-cell activation, LEUKEMIA, Vol: 24, Pages: 896-900, ISSN: 0887-6924

Journal article

Skobridis K, Kinigopoulou M, Theodorou V, Giannousi E, Russell A, Chauhan R, Sala R, Brownlow N, Kiriakidis S, Domin J, Tzakos AG, Dibb NJet al., 2010, Novel Imatinib Derivatives with Altered Specificity between Bcr-AbI and FMS, KIT, and PDGF Receptors, CHEMMEDCHEM, Vol: 5, Pages: 130-139, ISSN: 1860-7179

Journal article

Brownlow N, Mol C, Hayford C, Ghaem-Maghami S, Dibb NJet al., 2009, Dasatinib is a potent inhibitor of tumour-associated macrophages, osteoclasts and the FMS receptor, LEUKEMIA, Vol: 23, Pages: 590-594, ISSN: 0887-6924

Journal article

Brownlow N, Vaid M, Dibb NJ, 2008, Tandutinib inhibits FMS receptor signalling, and macrophage and osteoclast formation in vitro, LEUKEMIA, Vol: 22, Pages: 1452-1453, ISSN: 0887-6924

Journal article

Lo ASY, Taylor JR, Farzaneh F, Kemeny DM, Dibb NJ, Maher Jet al., 2008, Harnessing the tumour-derived cytokine, CSF-1, to co-stimulate T-cell growth and activation, MOLECULAR IMMUNOLOGY, Vol: 45, Pages: 1276-1287, ISSN: 0161-5890

Journal article

Brownlow N, Russell AE, Saravanapavan H, Wiesmann M, Murray JM, Manley PW, Dibb NJet al., 2008, Comparison of nilotinib and imatinib inhibition of FMS receptor signaling, macrophage production and osteoclastogenesis, LEUKEMIA, Vol: 22, Pages: 649-652, ISSN: 0887-6924

Journal article

Khorashad JS, Lipton JH, Marin D, Milojkovic D, Cross NCP, Dibb N, Melo JV, Kamel-Reid S, Goldman JM, Apperley JF, Kaeda JSet al., 2006, Abnormally small BCR-ABL transcripts in CML patients before and during imatinib treatment., 48th Annual Meeting of the American-Society-of-Hematology, Publisher: AMER SOC HEMATOLOGY, Pages: 611A-611A, ISSN: 0006-4971

Conference paper

Taylor JR, Brownlow N, Domin J, Dibb NJet al., 2006, FMS receptor for M-CSF (CSF-1) is sensitive to the kinase inhibitor imatinib and mutation of Asp-802 to Val confers resistance, ONCOGENE, Vol: 25, Pages: 147-151, ISSN: 0950-9232

Journal article

Dibb NJ, Dilworth SM, Mol CD, 2004, Opinion - Switching on kinases: oncogenic activation of BRAF and the PDGFR family, NATURE REVIEWS CANCER, Vol: 4, Pages: 718-727, ISSN: 1474-175X

Journal article

Sadusky T, Newman AJ, Dibb NJ, 2004, Exon junction sequences as cryptic splice sites: Implications for intron origin, CURRENT BIOLOGY, Vol: 14, Pages: 505-509, ISSN: 0960-9822

Journal article

Baker DA, Mille-Baker B, Wainwright SM, Ish-Horowicz D, Dibb NJet al., 2001, Mae mediates MAP kinase phosphorylation of Ets transcription factors in Drosophila, NATURE, Vol: 411, Pages: 330-334, ISSN: 0028-0836

Journal article

Taylor DM, Handyside AH, Ray PF, Dibb NJ, Winston RML, Ao Aet al., 2001, Quantitative measurement of transcript levels throughout human preimplantation development: analysis of hypoxanthine phosphoribosyl transferase, MOLECULAR HUMAN REPRODUCTION, Vol: 7, Pages: 147-154, ISSN: 1360-9947

Journal article

Maher J, Lo ASY, Dibb NJ, Farzaneh F, Sadelain M, Kemeny DMet al., 2000, Harnessing the tumor-derived cytokine, macrophage colony-stimulating factor (M-CSF), to costimulate genetically modified T cells., BLOOD, Vol: 96, Pages: 294A-294A, ISSN: 0006-4971

Journal article

Uden M, Morley GM, Dibb NJ, 1999, Evidence that downregulation of the M-CSF receptor is not dependent upon receptor kinase activity, ONCOGENE, Vol: 18, Pages: 3846-3851, ISSN: 0950-9232

Journal article

Morley GM, Uden M, Gullick WJ, Dibb NJet al., 1999, Cell specific transformation by c-fms activating loop mutations is attributable to constitutive receptor degradation, ONCOGENE, Vol: 18, Pages: 3076-3084, ISSN: 0950-9232

Journal article

Takeda S, Soutter WP, Dibb NJ, White JOet al., 1996, Biological activity of the receptor for macrophage colony-stimulating factor in the human endometrial cancer cell line, Ishikawa, BRITISH JOURNAL OF CANCER, Vol: 73, Pages: 615-619, ISSN: 0007-0920

Journal article

Maher J, Baker D, Dibb N, Roberts Iet al., 1996, Mutant ras promotes haemopoietic cell proliferation or differentiation in a cell-specific manner, LEUKEMIA, Vol: 10, Pages: 83-90, ISSN: 0887-6924

Journal article

Baker DA, Uden M, White JO, Dibb NJet al., 1995, M-CSF autocrine loop stimulates the proliferation of AML and CML leukaemic cell lines, BLOOD, Vol: 86, Pages: 2884-2884, ISSN: 0006-4971

Journal article

MAHER J, BAKER DA, MANNING M, DIBB NJ, ROBERTS IAGet al., 1995, EVIDENCE FOR CELL-SPECIFIC DIFFERENCES IN TRANSFORMATION BY N-RAS, H-RAS AND K-RAS, ONCOGENE, Vol: 11, Pages: 1639-1647, ISSN: 0950-9232

Journal article

GLOVER HR, BAKER DA, CELETTI A, DIBB NJet al., 1995, SELECTION OF ACTIVATING MUTATIONS OF C-FMS IN FDC-P1 CELLS, ONCOGENE, Vol: 11, Pages: 1347-1356, ISSN: 0950-9232

Journal article

MAHER J, BAKER DA, DIBB NJ, ROBERTS IAGet al., 1994, N-RAS, H-RAS, AND K-RAS - A DIFFERENCE IN TRANSFORMING POTENTIAL, BLOOD, Vol: 84, Pages: A295-A295, ISSN: 0006-4971

Journal article

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