Imperial College London
Alternate

DrNickDibb

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Honorary Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 2103n.dibb

 
 
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Location

 

5009Hammersmith HospitalHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Tan:2014:nar/gku656,
author = {Tan, GC and Chan, E and Molnar, A and Sarkar, R and Alexieva, D and Isa, IM and Robinson, S and Zhang, S and Ellis, P and Langford, CF and Guillot, PV and Chandrashekran, A and Fisk, NM and Castellano, L and Meister, G and Winston, RM and Cui, W and Baulcombe, D and Dibb, NJ},
doi = {nar/gku656},
journal = {Nucleic Acids Research},
pages = {9424--9435},
title = {5 ' isomiR variation is of functional and evolutionary importance},
url = {http://dx.doi.org/10.1093/nar/gku656},
volume = {42},
year = {2014}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - We have sequenced miRNA libraries from human embryonic,neural and foetal mesenchymal stem cells.We report that the majority of miRNA genes encodemature isomers that vary in size by one ormore bases at the 3 and/or 5 end of the miRNA.Northern blotting for individual miRNAs showed thatthe proportions of isomiRs expressed by a singlemiRNA gene often differ between cell and tissuetypes. IsomiRs were readily co-immunoprecipitatedwith Argonaute proteins in vivo and were active inluciferase assays, indicating that they are functional.Bioinformatics analysis predicts substantial differencesin targeting between miRNAs with minor 5differences and in support of this we report that a 5isomiR-9–1 gained the ability to inhibit the expressionof DNMT3B and NCAM2 but lost the ability toinhibit CDH1 in vitro. This result was confirmed bythe use of isomiR-specific sponges. Our analysis ofthe miRGator database indicates that a small percentageof human miRNA genes express isomiRs asthe dominant transcript in certain cell types and analysisof miRBase shows that 5 isomiRs have replacedcanonical miRNAs many times during evolution. Thisstrongly indicates that isomiRs are of functional importanceand have contributed to the evolution ofmiRNA genes.INT
AU  - Tan,GC
AU  - Chan,E
AU  - Molnar,A
AU  - Sarkar,R
AU  - Alexieva,D
AU  - Isa,IM
AU  - Robinson,S
AU  - Zhang,S
AU  - Ellis,P
AU  - Langford,CF
AU  - Guillot,PV
AU  - Chandrashekran,A
AU  - Fisk,NM
AU  - Castellano,L
AU  - Meister,G
AU  - Winston,RM
AU  - Cui,W
AU  - Baulcombe,D
AU  - Dibb,NJ
DO  - nar/gku656
EP  - 9435
PY  - 2014///
SN  - 1362-4962
SP  - 9424
TI  - 5 ' isomiR variation is of functional and evolutionary importance
T2  - Nucleic Acids Research
UR  - http://dx.doi.org/10.1093/nar/gku656
UR  - http://hdl.handle.net/10044/1/26890
VL  - 42
ER  -
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