Imperial College London
Alternate

DrNickDibb

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Honorary Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 2103n.dibb

 
 
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Location

 

5009Hammersmith HospitalHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@inbook{Chandrashekran:2016:10.1007/978-1-4939-3753-0_8,
author = {Chandrashekran, A and Casimir, C and Dibb, N and Readhead, C and Winston, R},
booktitle = {Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools.},
doi = {10.1007/978-1-4939-3753-0_8},
pages = {95--106},
publisher = {Humana Press},
title = {Generating Transgenic Mice by Lentiviral Transduction of Spermatozoa Followed by In Vitro Fertilization and Embryo Transfer.},
url = {http://dx.doi.org/10.1007/978-1-4939-3753-0_8},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - CHAP
AB  - Most transgenic technologies rely on the oocyte as a substrate for genetic modification. Transgenics animals are usually generated by the injection of the gene constructs (including lentiviruses encoding gene constructs or modified embryonic stem cells) into the pronucleus of a fertilized egg followed by the transfer of the injected embryos into the uterus of a foster mother. Male germ cells also have potential as templates for transgenic development. We have previously shown that mature sperm can be utilized as template for lentiviral transduction and as such used to generate transgenic mice efficiently with germ line capabilities. We provide here a detailed protocol that is relatively simple, to establish transgenic mice using lentivirally transduced spermatozoa. This protocol employs a well-established lentiviral gene delivery system (usual for somatic cells) delivering a variety of transgenes to be directly used with sperm, and the subsequent use of these modified sperm in in vitro fertilization studies and embryo transfer into foster female mice, for the establishment of transgenic mice.
AU  - Chandrashekran,A
AU  - Casimir,C
AU  - Dibb,N
AU  - Readhead,C
AU  - Winston,R
DO  - 10.1007/978-1-4939-3753-0_8
EP  - 106
PB  - Humana Press
PY  - 2016///
SN  - 978-1-4939-3751-6
SP  - 95
TI  - Generating Transgenic Mice by Lentiviral Transduction of Spermatozoa Followed by In Vitro Fertilization and Embryo Transfer.
T1  - Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools.
UR  - http://dx.doi.org/10.1007/978-1-4939-3753-0_8
UR  - https://www.ncbi.nlm.nih.gov/pubmed/27317176
ER  -
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