Imperial College London
Alternate

Dr Neil Dufton

Faculty of Medicine, National Heart & Lung Institute

Honorary Research Associate
 
 
 
//

Contact

 

+44 (0)20 7594 2728n.dufton

 
 
//

Location

 

525W5ICTEM buildingHammersmith Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Shah:2017:10.1038/ncomms16002,
author = {Shah, AV and Birdsey, GM and Peghaire, C and Pitulescu, ME and Dufton, NP and Yang, Y and Weinberg, I and Osuna, Almagro L and Payne, L and Mason, JC and Gerhardt, H and Adams, RH and Randi, AM},
doi = {10.1038/ncomms16002},
journal = {Nature Communications},
pages = {1--16},
title = {The endothelial transcription factor ERG mediates Angiopoietin-1-dependent control of Notch signalling and vascular stability},
url = {http://dx.doi.org/10.1038/ncomms16002},
volume = {8},
year = {2017}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Notch and Angiopoietin-1 (Ang1)/Tie2 pathways are crucial for vascular maturation and stability. Here we identify the transcription factor ERG as a key regulator of endothelial Notch signalling. We show that ERG controls the balance between Notch ligands by driving Delta-like ligand 4 (Dll4) while repressing Jagged1 (Jag1) expression. In vivo, this regulation occurs selectively in the maturing plexus of the mouse developing retina, where Ang1/Tie2 signalling is active. We find that ERG mediates Ang1-dependent regulation of Notch ligands and is required for the stabilizing effects of Ang1 in vivo. We show that Ang1 induces ERG phosphorylation in a phosphoinositide 3-kinase (PI3K)/Akt-dependent manner, resulting in ERG enrichment at Dll4 promoter and multiple enhancers. Finally, we demonstrate that ERG directly interacts with Notch intracellular domain (NICD) and β-catenin and is required for Ang1-dependent β-catenin recruitment at the Dll4 locus. We propose that ERG coordinates Ang1, β-catenin and Notch signalling to promote vascular stability.
AU  - Shah,AV
AU  - Birdsey,GM
AU  - Peghaire,C
AU  - Pitulescu,ME
AU  - Dufton,NP
AU  - Yang,Y
AU  - Weinberg,I
AU  - Osuna,Almagro L
AU  - Payne,L
AU  - Mason,JC
AU  - Gerhardt,H
AU  - Adams,RH
AU  - Randi,AM
DO  - 10.1038/ncomms16002
EP  - 16
PY  - 2017///
SN  - 2041-1723
SP  - 1
TI  - The endothelial transcription factor ERG mediates Angiopoietin-1-dependent control of Notch signalling and vascular stability
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/ncomms16002
UR  - https://www.nature.com/articles/ncomms16002
UR  - http://hdl.handle.net/10044/1/48569
VL  - 8
ER  -
Download