Imperial College London
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DrNiklasFeldhahn

Faculty of Medicine, Department of Immunology and Inflammation

Senior Lecturer in Molecular Haematology
 
 
 
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Contact

 

+44 (0)20 3313 1528n.feldhahn Website

 
 
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Location

 

, 4N3DCommonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Scheid:2009:10.1016/j.jim.2008.11.012,
author = {Scheid, JF and Mouquet, H and Feldhahn, N and Walker, BD and Pereyra, F and Cutrell, E and Seaman, MS and Mascola, JR and Wyatt, RT and Wardemann, H and Nussenzweig, MC},
doi = {10.1016/j.jim.2008.11.012},
journal = {J Immunol Methods},
pages = {65--67},
title = {A method for identification of HIV gp140 binding memory B cells in human blood},
url = {http://dx.doi.org/10.1016/j.jim.2008.11.012},
volume = {343},
year = {2009}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Antibodies to HIV are potentially important reagents for basic and clinical studies. Historically, these reagents have been produced by random cloning of heavy and light chains in phage display libraries [Burton, D.R., Barbas, C.F. III, Persson, M.A.A., Koenig, S., Chanock, R.M., and Lerner, R.A., (1991), A large array of human monoclonal antibodies to type 1 immunodeficiency virus from combinatorial libraries of asymptomatic seropositive individuals. Proc. Natl. Acad. Sci. U. S. A. 88, 10134-10137.] and electrofusion techniques [Buchacher, A., Predl, R., Tauer, C., Purtscher, M., Gruber, G., Heider, R., Steindl, F., Trkola, A., Jungbauer, A., and Katinger, H., (1992), Human monoclonal antibodies against gp41 and gp120 as potential agent for passive immunization. Vaccines 92, 191-195]. Here we describe a method to identify and potentially enrich human memory B cells from HIV infected patients that show serum titers of neutralizing antibodies. When biotinylated gp140 is used to stain peripheral blood mononuclear cells it identifies a distinct population of gp140 binding B cells by flow cytometry.
AU  - Scheid,JF
AU  - Mouquet,H
AU  - Feldhahn,N
AU  - Walker,BD
AU  - Pereyra,F
AU  - Cutrell,E
AU  - Seaman,MS
AU  - Mascola,JR
AU  - Wyatt,RT
AU  - Wardemann,H
AU  - Nussenzweig,MC
DO  - 10.1016/j.jim.2008.11.012
EP  - 67
PY  - 2009///
SN  - 1872-7905
SP  - 65
TI  - A method for identification of HIV gp140 binding memory B cells in human blood
T2  - J Immunol Methods
UR  - http://dx.doi.org/10.1016/j.jim.2008.11.012
UR  - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19100741
VL  - 343
ER  -
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