Imperial College London
Alternate

DrNicholasKirkby

Faculty of Medicine, National Heart & Lung Institute

Senior Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 3075n.kirkby

 
 
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Location

 

364Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@inbook{Akhmedov:2019:10.1007/978-1-4939-9121-1_21,
author = {Akhmedov, D and Kirkby, NS and Mitchell, JA and Berdeaux, R},
doi = {10.1007/978-1-4939-9121-1_21},
pages = {361--376},
title = {Imaging of Tissue-Specific and Temporal Activation of GPCR Signaling Using DREADD Knock-In Mice.},
url = {http://dx.doi.org/10.1007/978-1-4939-9121-1_21},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - CHAP
AB  - Engineered G protein-coupled receptors (DREADDs, designer receptors exclusively activated by designer drugs) are convenient tools for specific activation of GPCR signaling in many cell types. DREADDs have been utilized as research tools to study numerous cellular and physiologic processes, including regulation of neuronal activity, behavior, and metabolism. Mice with random insertion transgenes and adeno-associated viruses have been widely used to express DREADDs in individual cell types. We recently created and characterized ROSA26-GsDREADD knock-in mice to allow Cre recombinase-dependent expression of a Gαs-coupled DREADD (GsD) fused to GFP in distinct cell populations in vivo. These animals also harbor a CREB-activated luciferase reporter gene for analysis of CREB activity by in vivo imaging, ex vivo imaging, or biochemical reporter assays. In this chapter, we provide detailed methods for breeding GsD animals, inducing GsD expression, stimulating GsD activity, and measuring basal and stimulated CREB reporter bioluminescence in tissues in vivo, ex vivo, and in vitro. These animals are available from our laboratory for non-profit research.
AU  - Akhmedov,D
AU  - Kirkby,NS
AU  - Mitchell,JA
AU  - Berdeaux,R
DO  - 10.1007/978-1-4939-9121-1_21
EP  - 376
PY  - 2019///
SP  - 361
TI  - Imaging of Tissue-Specific and Temporal Activation of GPCR Signaling Using DREADD Knock-In Mice.
UR  - http://dx.doi.org/10.1007/978-1-4939-9121-1_21
UR  - https://www.ncbi.nlm.nih.gov/pubmed/30969428
ER  -
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