Imperial College London
Alternate

DrNicholasKirkby

Faculty of Medicine, National Heart & Lung Institute

Senior Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 3075n.kirkby

 
 
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Location

 

364Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Ahmetaj-Shala:2020:10.1096/fj.201901379RR,
author = {Ahmetaj-Shala, B and Kawai, R and Marei, I and Nikolakopoulou, Z and Shih, C-C and Konain, B and Reed, DM and Mongey, R and Kirkby, NS and Mitchell, JA},
doi = {10.1096/fj.201901379RR},
journal = {The FASEB Journal},
pages = {1745--1754},
title = {A bioassay system of autologous human endothelial, smooth muscle cells and leucocytes for use in drug discovery, phenotyping and tissue engineering},
url = {http://dx.doi.org/10.1096/fj.201901379RR},
volume = {34},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Purpose: Blood vessels are comprised of endothelial and smooth muscle cells. Obtaining both types of cells from vessels of living donors is not possible without invasive surgery. To address this we have devised a strategy whereby human endothelial and smooth muscle cells derived from blood progenitors from the same donor could be cultured with autologous leucocytes to generate a same donor ‘vessel in a dish’ bioassay. Basic procedures: Autologous sets of blood outgrowth endothelial cells (BOECs), smooth muscle cells (BO-SMCs) and leucocytes were obtained from 4 donors. Cells were treated in mono and cumulative co-culture conditions. The endothelial specific mediator endothelin-1 along with interleukin (IL)-6, IL-8, tumour necrosis factor α, and interferon gamma-induced protein 10 were measured under control culture conditions and after stimulation with cytokines.Main findings: Co-cultures remained viable throughout. The profile of individual mediators released from cells was consistent with what we know of endothelial and smooth muscle cells cultured from blood vessels.Principle conclusions: For the first time, we report a proof of concept study where autologous blood outgrowth ‘vascular’ cells and leucocytes were studied alone and in co-culture. This novel bioassay has utility in vascular biology research, patient phenotyping, drug testing and tissue engineering.
AU  - Ahmetaj-Shala,B
AU  - Kawai,R
AU  - Marei,I
AU  - Nikolakopoulou,Z
AU  - Shih,C-C
AU  - Konain,B
AU  - Reed,DM
AU  - Mongey,R
AU  - Kirkby,NS
AU  - Mitchell,JA
DO  - 10.1096/fj.201901379RR
EP  - 1754
PY  - 2020///
SN  - 0892-6638
SP  - 1745
TI  - A bioassay system of autologous human endothelial, smooth muscle cells and leucocytes for use in drug discovery, phenotyping and tissue engineering
T2  - The FASEB Journal
UR  - http://dx.doi.org/10.1096/fj.201901379RR
UR  - http://hdl.handle.net/10044/1/74886
VL  - 34
ER  -
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