Imperial College London
Alternate

DrNicholasKirkby

Faculty of Medicine, National Heart & Lung Institute

Senior Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 3075n.kirkby

 
 
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Location

 

364Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Lee:2017:10.1142/S0192415X17500884,
author = {Lee, S-Y and Chen, P-Y and Lin, J-C and Kirkby, NS and Ou, C-H and Chang, T-C},
doi = {10.1142/S0192415X17500884},
journal = {AMERICAN JOURNAL OF CHINESE MEDICINE},
pages = {1631--1648},
title = {Melaleuca alternifolia Induces Heme Oxygenase-1 Expression in Murine RAW264.7 Cells through Activation of the Nrf2-ARE Pathway},
url = {http://dx.doi.org/10.1142/S0192415X17500884},
volume = {45},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Melaleuca alternifolia concentrate (MAC) is the refined essential oil of the Australian native plant Melaleuca alternifolia. MAC has been reported to suppress the production of pro-inflammatory cytokines in both murine RAW264.7 macrophages and human monocytes stimulated with lipopolysaccharide (LPS). However, the mechanisms involved in this effect remain unclear. This study aims to delineate the molecular mechanisms that drive the anti-inflammatory activity of MAC and its active component, terpinen-4-ol, in macrophages. The effects of MAC on RAW264.7 cells were studied using western blotting, real-time PCR, an electrophoretic mobility shift assay (EMSA), and NF-κB luciferase reporter assays. Our results showed that MAC significantly increased both the mRNA and protein levels of heme oxygenase-1 (HO-1) via p38 and JNK MAPK activation. In addition, we showed that MAC significantly increased the activation and nuclear translocation of NF-E2-related factor 2 (Nrf2), a key transcription factor regulating HO-1 induction. MAC was also associated with significant inhibition of iNOS expression, NO production, and NF-κB activation. HO-1 was required for these anti-inflammatory effects as tin protoporphyrin IX (SnPPIX), an HO-1 inhibitor, abolished the effects of MAC on LPS-induced iNOS, NO, and NF-κB activation. Our results indicate that MAC induces HO-1 expression in murine macrophages via the p38 MAPK and JNK pathways and that this induction is required for its anti-inflammatory activity.
AU  - Lee,S-Y
AU  - Chen,P-Y
AU  - Lin,J-C
AU  - Kirkby,NS
AU  - Ou,C-H
AU  - Chang,T-C
DO  - 10.1142/S0192415X17500884
EP  - 1648
PY  - 2017///
SN  - 0192-415X
SP  - 1631
TI  - Melaleuca alternifolia Induces Heme Oxygenase-1 Expression in Murine RAW264.7 Cells through Activation of the Nrf2-ARE Pathway
T2  - AMERICAN JOURNAL OF CHINESE MEDICINE
UR  - http://dx.doi.org/10.1142/S0192415X17500884
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000417698800004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/62053
VL  - 45
ER  -
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