Imperial College London
Alternate

DrNobuakiKudo

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Honorary Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 3803n.kudo Website

 
 
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Assistant

 

Miss Suzy Liu +44 (0)20 7594 2135

 
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Location

 

1 006Institute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kim:2013:10.1007/978-1-62703-191-2_3,
author = {Kim, JH and Ishiguro, KI and Kudo, N and Watanabe, Y},
doi = {10.1007/978-1-62703-191-2_3},
journal = {Methods in Molecular Biology},
pages = {47--57},
title = {Studying meiosis-specific cohesins in mouse embryonic oocytes},
url = {http://dx.doi.org/10.1007/978-1-62703-191-2_3},
volume = {957},
year = {2013}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Distinct meiotic cohesin complexes play fundamental roles in various meiosis-specific chromosomal events in spatiotemporally different manners during mammalian meiotic prophase. Immunostaining is one of the essential methods to study meiotic cohesin dynamics. For the study of cohesins in the meiotic prophase of oocytes, ovaries should be taken from the embryos during a very limited period before birth. Here we focus on some technical tips concerning the preparation of oocyte chromosome spreads for immunostaining. Further, we describe a method for chromosome fluorescence in situ hybridization (FISH) against immunostained oocytes. © 2013 Springer Science+Business Media, LLC.
AU  - Kim,JH
AU  - Ishiguro,KI
AU  - Kudo,N
AU  - Watanabe,Y
DO  - 10.1007/978-1-62703-191-2_3
EP  - 57
PY  - 2013///
SN  - 1064-3745
SP  - 47
TI  - Studying meiosis-specific cohesins in mouse embryonic oocytes
T2  - Methods in Molecular Biology
UR  - http://dx.doi.org/10.1007/978-1-62703-191-2_3
VL  - 957
ER  -
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