Imperial College London
Alternate

DrNobuakiKudo

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Honorary Research Fellow
 
 
 
//

Contact

 

+44 (0)20 7594 3803n.kudo Website

 
 
//

Assistant

 

Miss Suzy Liu +44 (0)20 7594 2135

 
//

Location

 

1 006Institute of Reproductive and Developmental BiologyHammersmith Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Wirth:2006:10.1083/jcb.200506119,
author = {Wirth, KG and Wutz, G and Kudo, NR and Desdouets, C and Zetterberg, A and Taghybeeglu, S and Seznec, J and Ducos, GM and Ricci, R and Firnberg, N and Peters, JM and Nasmyth, K},
doi = {10.1083/jcb.200506119},
journal = {The Journal of Cell Biology},
pages = {847--860},
title = {Separase: a universal trigger for sister chromatid disjunction but not chromosome cycle progression},
url = {http://dx.doi.org/10.1083/jcb.200506119},
volume = {172},
year = {2006}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Separase is a protease whose liberation from its inhibitory chaperone Securin triggers sister chromatid disjunction at anaphase onset in yeast by cleaving cohesin's kleisin subunit. We have created conditional knockout alleles of the mouse Separase and Securin genes. Deletion of both copies of Separase but not Securin causes embryonic lethality. Loss of Securin reduces Separase activity because deletion of just one copy of the Separase gene is lethal to embryos lacking Securin. In embryonic fibroblasts, Separase depletion blocks sister chromatid separation but does not prevent other aspects of mitosis, cytokinesis, or chromosome replication. Thus, fibroblasts lacking Separase become highly polyploid. Hepatocytes stimulated to proliferate in vivo by hepatectomy also become unusually large and polyploid in the absence of Separase but are able to regenerate functional livers. Separase depletion in bone marrow causes aplasia and the presumed death of hematopoietic cells other than erythrocytes. Destruction of sister chromatid cohesion by Separase may be a universal feature of mitosis in eukaryotic cells.
AU  - Wirth,KG
AU  - Wutz,G
AU  - Kudo,NR
AU  - Desdouets,C
AU  - Zetterberg,A
AU  - Taghybeeglu,S
AU  - Seznec,J
AU  - Ducos,GM
AU  - Ricci,R
AU  - Firnberg,N
AU  - Peters,JM
AU  - Nasmyth,K
DO  - 10.1083/jcb.200506119
EP  - 860
PY  - 2006///
SN  - 0021-9525
SP  - 847
TI  - Separase: a universal trigger for sister chromatid disjunction but not chromosome cycle progression
T2  - The Journal of Cell Biology
UR  - http://dx.doi.org/10.1083/jcb.200506119
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000235971900011&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://rupress.org/jcb/article/172/6/847/44679/Separase-a-universal-trigger-for-sister-chromatid
UR  - http://hdl.handle.net/10044/1/84204
VL  - 172
ER  -
Download