Imperial College London
Alternate

DrNobuakiKudo

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Honorary Research Fellow
 
 
 
//

Contact

 

+44 (0)20 7594 3803n.kudo Website

 
 
//

Assistant

 

Miss Suzy Liu +44 (0)20 7594 2135

 
//

Location

 

1 006Institute of Reproductive and Developmental BiologyHammersmith Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Amouroux:2016:10.1038/ncb3296,
author = {Amouroux, R and Nashun, B and Shirane, K and Nakagawa, S and Hill, PW and D'Souza, Z and Nakayama, M and Matsuda, M and Turp, A and Ndjetehe, E and Encheva, V and Kudo, NR and Koseki, H and Sasaki, H and Hajkova, P},
doi = {10.1038/ncb3296},
journal = {Nature Cell Biology},
pages = {225--233},
title = {De novo DNA methylation drives 5hmC accumulation in mouse zygotes.},
url = {http://dx.doi.org/10.1038/ncb3296},
volume = {18},
year = {2016}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Zygotic epigenetic reprogramming entails genome-wide DNA demethylation that is accompanied by Tet methylcytosine dioxygenase 3 (Tet3)-driven oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC; refs ,,,). Here we demonstrate using detailed immunofluorescence analysis and ultrasensitive LC-MS-based quantitative measurements that the initial loss of paternal 5mC does not require 5hmC formation. Small-molecule inhibition of Tet3 activity, as well as genetic ablation, impedes 5hmC accumulation in zygotes without affecting the early loss of paternal 5mC. Instead, 5hmC accumulation is dependent on the activity of zygotic Dnmt3a and Dnmt1, documenting a role for Tet3-driven hydroxylation in targeting de novo methylation activities present in the early embryo. Our data thus provide further insights into the dynamics of zygotic reprogramming, revealing an intricate interplay between DNA demethylation, de novo methylation and Tet3-driven hydroxylation.
AU  - Amouroux,R
AU  - Nashun,B
AU  - Shirane,K
AU  - Nakagawa,S
AU  - Hill,PW
AU  - D'Souza,Z
AU  - Nakayama,M
AU  - Matsuda,M
AU  - Turp,A
AU  - Ndjetehe,E
AU  - Encheva,V
AU  - Kudo,NR
AU  - Koseki,H
AU  - Sasaki,H
AU  - Hajkova,P
DO  - 10.1038/ncb3296
EP  - 233
PY  - 2016///
SN  - 1476-4679
SP  - 225
TI  - De novo DNA methylation drives 5hmC accumulation in mouse zygotes.
T2  - Nature Cell Biology
UR  - http://dx.doi.org/10.1038/ncb3296
UR  - https://www.nature.com/articles/ncb3296
VL  - 18
ER  -
Download