Imperial College London
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PROFESSOR NICHOLAS LONG

Faculty of Natural Sciences, Department of Chemistry

Sir Edward Frankland BP Chair -Inorganic Chemistry
 
 
 
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Contact

 

+44 (0)20 7594 5781n.long Website CV

 
 
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Location

 

501jMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Boltersdorf:2019:10.1039/c9dt03520f,
author = {Boltersdorf, T and Ansari, J and Senchenkova, EY and Jiang, L and White, AJP and Coogan, M and Gavins, FNE and Long, NJ},
doi = {10.1039/c9dt03520f},
journal = {Dalton Trans},
pages = {16764--16775},
title = {Development, characterisation and in vitro evaluation of lanthanide-based FPR2/ALX-targeted imaging probes.},
url = {http://dx.doi.org/10.1039/c9dt03520f},
volume = {48},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - We report the design, preparation and characterisation of three small-molecule, Formyl Peptide Receptor (FPR)-targeted lanthanide complexes (Tb·14, Eu·14 and Gd·14). Long-lived, metal-based emission was observed from the terbium complex (τH2O = 1.9 ms), whereas only negligible lanthanide signals were detected in the europium analogue. Ligand-centred emission was investigated using Gd·14 at room temperature and 77 K, leading to the postulation that metal emission may be sensitised via a ligand-based charge transfer state of the targeting Quin C1 unit. Comparatively high longitudinal relaxivity values (r1) for octadentate metal complexes of Gd·14 were determined (6.9 mM-1 s-1 at 400 MHz and 294 K), which could be a result of a relative increase in twisted square antiprism (TSAP) isomer prevalence compared to typical DOTA constructs (as evidenced by NMR spectroscopy). In vitro validation of concentration responses of Tb·14via three key neutrophil functional assays demonstrated that the inflammatory responses of neutrophils (i.e. chemotaxis, transmigration and granular release) remained unchanged in the presence of specific concentrations of the compound. Using a time-resolved microscopy set-up we were able to observe binding of the Tb·14 probe to stimulated human neutrophils around the cell periphery, while in the same experiment with un-activated neutrophils, no metal-based signals were detected. Our results demonstrate the utility of Tb·14 for time-resolved microscopy with lifetimes several orders of magnitude longer than autofluorescent signals and a preferential uptake in stimulated neutrophils.
AU  - Boltersdorf,T
AU  - Ansari,J
AU  - Senchenkova,EY
AU  - Jiang,L
AU  - White,AJP
AU  - Coogan,M
AU  - Gavins,FNE
AU  - Long,NJ
DO  - 10.1039/c9dt03520f
EP  - 16775
PY  - 2019///
SP  - 16764
TI  - Development, characterisation and in vitro evaluation of lanthanide-based FPR2/ALX-targeted imaging probes.
T2  - Dalton Trans
UR  - http://dx.doi.org/10.1039/c9dt03520f
UR  - https://www.ncbi.nlm.nih.gov/pubmed/31674608
VL  - 48
ER  -
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