Publications
143 results found
Lees NJ, Rosenberg AJP, Hurtado-Doce AI, et al., 2016, Combination of ECMO and cytokine adsorption therapy for severe sepsis with cardiogenic shock and ARDS due to Panton-Valentine leukocidin-positive Staphylococcus aureus pneumonia and H1N1, JOURNAL OF ARTIFICIAL ORGANS, Vol: 19, Pages: 399-402, ISSN: 1434-7229
Tatham KC, O'Dea KP, Romana R, et al., 2016, Retention And Activation Of Donor Vascular Monocytes In Transplanted Lungs Suggests A Central Role In Primary Graft Dysfunction, American Thoracic Society Conference 2016
Tatham KC, O'Dea KP, Romano R, et al., 2016, Retention And Activation Of Donor Vascular Monocytes In Transplanted Lungs Suggests A Central Role In Primary Graft Dysfunction, International Conference of the American-Thoracic-Society (ATS), Publisher: AMER THORACIC SOC, ISSN: 1073-449X
Fisher AJ, Yonan N, Mascaro J, et al., 2016, A Study of Donor Ex-Vivo Lung Perfusion in UK Lung Transplantation (DEVELOP-UK), 36th Annual Meeting and Scientific Sessions of the International-Society-for-Heart-and-Lung-Transplantation (ISHLT), Publisher: ELSEVIER SCIENCE INC, Pages: S80-S80, ISSN: 1053-2498
Andreasson A, Yonan N, Fildes JE, et al., 2016, Profiling Biomarkers of Inflammation and Tissue Injury during Human Ex Vivo Lung Perfusion in the DEVELOP-UK Study, 36th Annual Meeting and Scientific Sessions of the International-Society-for-Heart-and-Lung-Transplantation (ISHLT), Publisher: ELSEVIER SCIENCE INC, Pages: S88-S88, ISSN: 1053-2498
Thakuria L, Romano R, Koster G, et al., 2016, Altered Surfactant Turn-Over Immediately after Lung Transplantation Is Associated with Impaired Graft Function at Three Months, 36th Annual Meeting and Scientific Sessions of the International-Society-for-Heart-and-Lung-Transplantation (ISHLT), Publisher: Elsevier, Pages: S231-S232, ISSN: 1053-2498
Mohite PN, Sabashnikov A, Patil NP, et al., 2016, The role of cardiopulmonary bypass in lung transplantation, CLINICAL TRANSPLANTATION, Vol: 30, Pages: 202-209, ISSN: 0902-0063
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- Citations: 21
Korovesi I, Kotanidou A, Papadomichelakis E, et al., 2016, Exhaled nitric oxide and carbon monoxide in mechanically ventilated brain-injured patients, JOURNAL OF BREATH RESEARCH, Vol: 10, ISSN: 1752-7155
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- Citations: 2
Marczin N, Popov AF, Zych B, et al., 2016, Outcomes of minimally invasive lung transplantation in a single centre: the routine approach for the future or do we still need clamshell incision?, Interactive Cardiovascular and Thoracic Surgery, Vol: 22, Pages: 537-545, ISSN: 1569-9293
OBJECTIVES: Minimally invasive lung transplantation (MILT) via bilateral anterior thoracotomies has emerged as a novel surgical strategy with potential patient benefits when compared with transverse thoracosternotomy (clamshell incision, CS). The aim of this study is to compare MILT with CS by focusing on operative characteristics, postoperative organ function and support and mid-term clinical outcomes at Harefield Hospital. METHODS: It was a retrospective observational study evaluating all bilateral sequential lung transplants between April 2010 and November 2013. RESULTS: CS was performed in 124 patients and MILT in 70 patients. Skin-to-skin surgical time was less in the MILT group [285 (265, 339) min] compared with CS [380 (306, 565) min] and MILT-cardiopulmonary bypass [426 (360, 478) min]. Ischaemic time was significantly longer (502 ± 116 vs 395 ± 145 min) in the MILT group compared with CS (P < 0.01). Early postoperative physiological variables were similar between groups. Patients in the MILT group required less blood [2 (0, 4) vs 3 (1, 5) units, P = 0.16] and platelet transfusion [0 (0, 1) vs 1 (0, 2) units, P < 0.01]. The median duration of mechanical ventilation was shorter (26 vs 44 h, P < 0.01) and intensive therapy unit stay was 2 days shorter (5 vs 7) in the MILT group. While overall survival was similar, fraction of expired volume in 1 s (FEV1) and forced vital capacity (FVC) were consistently higher in the MILT group compared with CS during mid-term follow-up after transplantation. Specifically, FEV1 and FVC were, respectively, 86 ± 21 and 88 ± 18% predicted in the MILT group compared with 74 ± 21 and 74 ± 19% predicted in the CS group (P < 0.01) at the 6-month follow-up. CONCLUSIONS: MILT was successfully introduced at our centre as a novel operative strategy. Despite longer ischaemic times and a more complex operation and management, MILT appears to offer early postoperative and mid-term clinic
Thakuria L, Romano R, Goss V, et al., 2016, Human Lungs Actively Synthesize And Secrete A Range Of Phospholipids Into The Airspace And Circulation In An Isolated Perfusion Model, International Conference of the American-Thoracic-Society (ATS), Publisher: AMER THORACIC SOC, ISSN: 1073-449X
Madsen J, Rideout S, Thakuria L, et al., 2016, Levels Of Surfactant Protein D In Bronchial Aspirates Within Six Hours Of Lung Transplantation Are Associated With Clinical Outcomes, International Conference of the American-Thoracic-Society (ATS), Publisher: AMER THORACIC SOC, ISSN: 1073-449X
Thakuria L, Davey R, Romano R, et al., 2015, Mechanical ventilation after lung transplantation, Journal of Critical Care, Vol: 31, Pages: 110-118, ISSN: 0883-9441
Introduction: To explore the hypothesis that early ventilation strategies influence clinical outcomes in lung transplantation,we have examined our routine ventilation practices in terms of tidal volumes (Vt) and inflation pressures.Methods: A total of 124 bilateral lung transplants between 2010 and 2013 were retrospectively assigned to low(b6 mL/kg), medium (6-8 mL/kg), and high (N8 mL/kg) Vt groups based on ventilation characteristics duringthe first 6 hours after surgery. Those same 124 patients were also stratified to low-pressure (b25 cm H2O) andhigh-pressure (≥25 cm H2O) groups.Results: Eighty percent of patients were ventilated using pressure control mode. Low, medium, and high Vt wereapplied to 10%, 43%, and 47% of patients, respectively. After correcting for patients requiring extracorporeal support,there was no difference in short-term to midterm outcomes among the different Vt groups. Low inflationpressures were applied to 61% of patients, who had a shorter length of intensive care unit stay (5 vs 12 days;P = .012), higher forced expiratory volume in 1 second at 3 months (77.8% vs 60.3%; P b .001), and increased6-month survival rate (95% vs 77%; P = .008).Conclusion: Low Vt ventilation has not been fully adopted in our practice. Ventilation with higher inflation pressures,but not Vt, was significantly associated with poorer outcomes after lung transplantation.
Benedetto M, Romano R, Baca G, et al., 2015, Inhaled nitric oxide in cardiac surgery: Evidence or tradition?, NITRIC OXIDE-BIOLOGY AND CHEMISTRY, Vol: 49, Pages: 67-79, ISSN: 1089-8603
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- Citations: 22
Fletcher ME, Boshier PR, Wakabayashi K, et al., 2015, Influence of glutathione-S-transferase (GST) inhibition on lung epithelial cell injury: role of oxidative stress and metabolism, AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, Vol: 308, Pages: L1274-L1285, ISSN: 1040-0605
Oxidant-mediated tissue injury is key to the pathogenesis of acute lung injury. Glutathione-S-transferases (GSTs) are important detoxifying enzymes that catalyze the conjugation of glutathione with toxic oxidant compounds and are associated with acute and chronic inflammatory lung diseases. We hypothesized that attenuation of cellular GST enzymes would augment intracellular oxidative and metabolic stress and induce lung cell injury. Treatment of murine lung epithelial cells with GST inhibitors, ethacrynic acid (EA), and caffeic acid compromised lung epithelial cell viability in a concentration-dependent manner. These inhibitors also potentiated cell injury induced by hydrogen peroxide (H2O2), tert-butyl-hydroperoxide, and hypoxia and reoxygenation (HR). SiRNA-mediated attenuation of GST-π but not GST-μ expression reduced cell viability and significantly enhanced stress (H2O2/HR)-induced injury. GST inhibitors also induced intracellular oxidative stress (measured by dihydrorhodamine 123 and dichlorofluorescein fluorescence), caused alterations in overall intracellular redox status (as evidenced by NAD+/NADH ratios), and increased protein carbonyl formation. Furthermore, the antioxidant N-acetylcysteine completely prevented EA-induced oxidative stress and cytotoxicity. Whereas EA had no effect on mitochondrial energetics, it significantly altered cellular metabolic profile. To explore the physiological impact of these cellular events, we used an ex vivo mouse-isolated perfused lung model. Supplementation of perfusate with EA markedly affected lung mechanics and significantly increased lung permeability. The results of our combined genetic, pharmacological, and metabolic studies on multiple platforms suggest the importance of GST enzymes, specifically GST-π, in the cellular and whole lung response to acute oxidative and metabolic stress. These may have important clinical implications.
Neil DA, Andreasson A, Roman M, et al., 2015, Ultrastructural Changes in Epithelial and Endothelial Barriers During Ex-Vivo Lung Perfusion, 35th Annual Meeting and Scientific Sessions of the International-Society-for-Heart-and-Lung-Transplantation, Publisher: ELSEVIER SCIENCE INC, Pages: S283-S283, ISSN: 1053-2498
Boshier PR, Mistry V, Cushnir JR, et al., 2015, Breath metabolite response to major upper gastrointestinal surgery, JOURNAL OF SURGICAL RESEARCH, Vol: 193, Pages: 704-712, ISSN: 0022-4804
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- Citations: 4
Tatham KC, O'Dea KP, Wakabayashi K, et al., 2015, The role of ex vivo lung perfusion in lung transplantation., J Intensive Care Soc, Vol: 16, Pages: 58-63, ISSN: 1751-1437
Whilst lung transplantation is a viable solution for end-stage lung disease, donor shortages, donor lung inflammation and perioperative lung injury remain major limitations. Ex vivo lung perfusion has emerged as the next frontier in lung transplantation to address and overcome these limitations, with multicentre clinical trials ongoing in the UK, rest of Europe and North America. Our research seeks to identify the poorly understood cellular and molecular mechanisms of primary graft dysfunction through the development of an isolated perfused lung model of transplantation and investigation of the role of pulmonary inflammation in this paradigm.
Boshier PR, Marczin N, Hanna GB, 2014, Pathophysiology of acute lung injury following esophagectomy, DISEASES OF THE ESOPHAGUS, Vol: 28, Pages: 797-804, ISSN: 1120-8694
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- Citations: 9
Cristescu SM, Kiss R, Hekkert STL, et al., 2014, Real-time monitoring of endogenous lipid peroxidation by exhaled ethylene in patients undergoing cardiac surgery, AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, Vol: 307, Pages: L509-L515, ISSN: 1040-0605
Pulmonary and systemic organ injury produced by oxidative stress including lipid peroxidation is a fundamental tenet of ischemia-reperfusion injury, inflammatory response to cardiac surgery, and cardiopulmonary bypass (CPB) but is not routinely measured in a surgically relevant time frame. To initiate a paradigm shift toward noninvasive and real-time monitoring of endogenous lipid peroxidation, we have explored pulmonary excretion and dynamism of exhaled breath ethylene during cardiac surgery to test the hypothesis that surgical technique and ischemia-reperfusion triggers lipid peroxidation. We have employed laser photoacoustic spectroscopy to measure real-time trace concentrations of ethylene from the patient breath and from the CPB machine. Patients undergoing aortic or mitral valve surgery-requiring CPB (n = 15) or off-pump coronary artery bypass surgery (OPCAB) (n = 7) were studied. Skin and tissue incision by diathermy caused striking (>30-fold) increases in exhaled ethylene resulting in elevated levels until CPB. Gaseous ethylene in the CPB circuit was raised upon the establishment of CPB (>10-fold) and decreased over time. Reperfusion of myocardium and lungs did not appear to enhance ethylene levels significantly. During OPCAB surgery, we have observed increased ethylene in 16 of 30 documented reperfusion events associated with coronary and aortic anastomoses. Therefore, novel real-time monitoring of endogenous lipid peroxidation in the intraoperative setting provides unparalleled detail of endogenous and surgery-triggered production of ethylene. Diathermy and unprotected regional myocardial ischemia and reperfusion are the most significant contributors to increased ethylene.
Mohite PN, Sabashnikov A, Patil NP, et al., 2014, Short-term ventricular assist device in post-cardiotomy cardiogenic shock: factors influencing survival, JOURNAL OF ARTIFICIAL ORGANS, Vol: 17, Pages: 228-235, ISSN: 1434-7229
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- Citations: 18
Thakuria L, Davey R, Romano R, et al., 2014, Inflation pressures are associated with outcomes in ventilated patients after lung transplantation, The European Respiratory Society Annual Meeting, Pages: P259-P259, ISSN: 1399-3003
Vives M, Wijeysundera D, Marczin N, et al., 2014, Cardiac surgery-associated acute kidney injury, INTERACTIVE CARDIOVASCULAR AND THORACIC SURGERY, Vol: 18, Pages: 637-645, ISSN: 1569-9293
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- Citations: 77
Romano R, Wong I, Thakuria L, et al., 2014, Influence of Intraoperative Hemodynamic Factors on the Development of Acute Kidney Injury in Lung Transplantation, 34th Annual Meeting and Scientific Sessions of the International-Society-for-Heart-and-Lung-Transplantation, Publisher: ELSEVIER SCIENCE INC, Pages: S192-S192, ISSN: 1053-2498
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- Citations: 1
Kovacs V, Gasz B, Balatonyi B, et al., 2014, Polymorphisms in glutathione S-transferase are risk factors for perioperative acute myocardial infarction after cardiac surgery: a preliminary study, MOLECULAR AND CELLULAR BIOCHEMISTRY, Vol: 389, Pages: 79-84, ISSN: 0300-8177
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- Citations: 8
Romano R, Wong I, Thakuria L, et al., 2014, Impact of Minimally Invasive Lung Transplantation on Postoperative Renal Outcomes, 34th Annual Meeting and Scientific Sessions of the International-Society-for-Heart-and-Lung-Transplantation, Publisher: ELSEVIER SCIENCE INC, Pages: S295-S296, ISSN: 1053-2498
Donaldson H, Tatham K, Odea K, et al., 2014, Subclinical endotoxaemia enhances lung ischaemia-reperfusion injury, Winter Meeting of the Anaesthetic-Research-Society (ARS), Publisher: OXFORD UNIV PRESS, Pages: 184P-185P, ISSN: 0007-0912
Mohite PN, Zych B, Popov AF, et al., 2013, CentriMag® short-term ventricular assist as a bridge to solution in patients with advanced heart failure: use beyond 30 days, EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY, Vol: 44, Pages: E310-E315, ISSN: 1010-7940
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- Citations: 40
Priest O, Bundy R, Marczin N, et al., 2013, N-acetyl cysteine inhibits human oesophagogastric adenocarcinoma cell growth and increases chemosensitivity via reduced HO activity and induction of apoptosis, 17th Annual Scientific Meeting of the Association-of-Upper-Gastrointestinal-Surgeons-of-Great-Britain-and-Ireland (AUGIS), Publisher: WILEY-BLACKWELL, Pages: 59-59, ISSN: 0007-1323
Tatham K, Donaldson H, O'Dea K, et al., 2013, Marginated monocytes play a central role in lung ischaemia-reperfusion injury in mice: Implications for lung transplantation, EUROPEAN RESPIRATORY JOURNAL, Vol: 42, ISSN: 0903-1936
Balatonyi B, Gasz B, Kovacs V, et al., 2013, The role of the inhibition of glutathione-<i>S</i>-transferase in the protective mechanisms of ischemic postconditioning, CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, Vol: 91, Pages: 625-632, ISSN: 0008-4212
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- Citations: 4
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