Imperial College London
Portrait Picture

Dr Niamh Nowlan

Faculty of Engineering, Department of Bioengineering

Visiting Reader
 
 
 
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Contact

 

+44 (0)20 7594 5189n.nowlan Website

 
 
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Location

 

4.10Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Giorgi:2019:10.1371/journal.pone.0197947,
author = {Giorgi, M and Sotiriou, V and Fanchini, N and Conigliaro, S and Bignardi, C and Nowlan, NC and Dall'Ara, E},
doi = {10.1371/journal.pone.0197947},
journal = {PLoS One},
pages = {1--11},
title = {Prenatal growth map of the mouse knee joint by means of deformable registration technique},
url = {http://dx.doi.org/10.1371/journal.pone.0197947},
volume = {14},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Joint morphogenesis is the process during which distinct and functional joint shapes emerge during pre- and post-natal joint development. In this study, a repeatable semi-automatic protocol capable of providing a 3D realistic developmental map of the prenatal mouse knee joint was designed by combining Optical Projection Tomography imaging (OPT) and a deformable registration algorithm (Sheffield Image Registration toolkit, ShIRT). Eleven left limbs of healthy murine embryos were scanned with OPT (voxel size: 14.63μm) at two different stages of development: Theiler stage (TS) 23 (approximately 14.5 embryonic days) and 24 (approximately 15.5 embryonic days). One TS23 limb was used to evaluate the precision of the displacement predictions for this specific case. The remaining limbs were then used to estimate Developmental Tibia and Femur Maps. Acceptable uncertainties of the displacement predictions computed from repeated images were found for both epiphyses (between 1.3μm and 1.4μm for the proximal tibia and between 0.7μm and 1.0μm for the femur, along all directions). The protocol was found to be reproducible with maximum Modified Housdorff Distance (MHD) differences equal to 1.9 μm and 1.5 μm for the tibial and femoral epiphyses respectively. The effect of the initial shape of the rudiment affected the developmental maps with MHD of 21.7 μm and 21.9 μm for the tibial and femoral epiphyses respectively, which correspond to 1.4 and 1.5 times the voxel size. To conclude, this study proposes a repeatable semi-automatic protocol capable of providing mean 3D realistic developmental map of a developing rudiment allowing researchers to study how growth and adaptation are directed by biological and mechanobiological factors.
AU  - Giorgi,M
AU  - Sotiriou,V
AU  - Fanchini,N
AU  - Conigliaro,S
AU  - Bignardi,C
AU  - Nowlan,NC
AU  - Dall'Ara,E
DO  - 10.1371/journal.pone.0197947
EP  - 11
PY  - 2019///
SN  - 1932-6203
SP  - 1
TI  - Prenatal growth map of the mouse knee joint by means of deformable registration technique
T2  - PLoS One
UR  - http://dx.doi.org/10.1371/journal.pone.0197947
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000454759900001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0197947
UR  - http://hdl.handle.net/10044/1/76570
VL  - 14
ER  -
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