13 results found
Stejskalova A, Oliva Jorge N, England F, et al., 2019, Biologically inspired, cell-selective release of aptamer-trapped growth factors by traction forces, Advanced Materials, Vol: 31, ISSN: 0935-9648
Biomaterial scaffolds that are designed to incorporate dynamic, spatiotemporal information have the potential to interface with cells and tissues to direct behavior. Here we describe a bioinspired, programmable nanotechnology-based platform that harnesses cellular traction forces to activate growth factors, eliminating the need for exogenous triggers (e.g. light), spatially diffuse triggers (e.g. enzymes, pH changes) or passive activation (e.g. hydrolysis). We use flexible aptamer technology to create modular, synthetic mimics of the Large Latent Complex that restrains TGF-β1. This flexible nanotechnology-based approach is shown here to work with both platelet-derived growth factor-BB (PDGF-BB) and vascular endothelial growth factor (VEGF-165), integrate with glass coverslips, polyacrylamide gels, and collagen scaffolds, enable activation by various cells (e.g. primary human dermal fibroblasts, HMEC-1 endothelial cells) and unlock fundamentally new capabilities such as selective activation of growth factors by differing cell types (e.g. activation by smooth muscle cells but not fibroblasts) within clinically relevant collagen sponges.
Conde J, Oliva N, Zhang Y, et al., 2016, Local triple-combination therapy results in tumour regression and prevents recurrence in a colon cancer model, NATURE MATERIALS, Vol: 15, Pages: 1128-+, ISSN: 1476-1122
Gilam A, Conde J, Weissglas-Volkov D, et al., 2016, Local microRNA delivery targets Palladin and prevents metastatic breast cancer, NATURE COMMUNICATIONS, Vol: 7, ISSN: 2041-1723
Conde J, Oliva N, Artzi N, 2016, Revisiting the 'One Material Fits All' Rule for Cancer Nanotherapy, TRENDS IN BIOTECHNOLOGY, Vol: 34, Pages: 618-626, ISSN: 0167-7799
Conde J, Oliva N, Atilano M, et al., 2016, Self-assembled RNA-triple-helix hydrogel scaffold for microRNA modulation in the tumour microenvironment, NATURE MATERIALS, Vol: 15, Pages: 353-+, ISSN: 1476-1122
Oliva N, Unterman S, Zhang Y, et al., 2015, Personalizing Biomaterials for Precision Nanomedicine Considering the Local Tissue Microenvironment, ADVANCED HEALTHCARE MATERIALS, Vol: 4, Pages: 1584-1599, ISSN: 2192-2640
Conde J, Oliva N, Artzi N, 2015, Implantable hydrogel embedded dark-gold nanoswitch as a theranostic probe to sense and overcome cancer multidrug resistance, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 112, Pages: E1278-E1287, ISSN: 0027-8424
Oliva N, Carcole M, Beckerman M, et al., 2015, Regulation of dendrimer/dextran material performance by altered tissue microenvironment in inflammation and neoplasia, SCIENCE TRANSLATIONAL MEDICINE, Vol: 7, ISSN: 1946-6234
Segovia N, Pont M, Oliva N, et al., 2015, Hydrogel Doped with Nanoparticles for Local Sustained Release of siRNA in Breast Cancer, ADVANCED HEALTHCARE MATERIALS, Vol: 4, Pages: 271-280, ISSN: 2192-2640
Oliva N, Shitreet S, Abraham E, et al., 2012, Natural Tissue Microenvironmental Conditions Modulate Adhesive Material Performance, LANGMUIR, Vol: 28, Pages: 15402-15409, ISSN: 0743-7463
Artzi N, Oliva N, Puron C, et al., 2011, In vivo and in vitro tracking of erosion in biodegradable materials using non-invasive fluorescence imaging (vol 10, pg 704, 2011), NATURE MATERIALS, Vol: 10, Pages: 896-896, ISSN: 1476-1122
Artzi N, Oliva N, Puron C, et al., 2011, In vivo and in vitro tracking of erosion in biodegradable materials using non-invasive fluorescence imaging, Nature Materials, Vol: 10, Pages: 704-709, ISSN: 1476-1122
The design of erodible biomaterials relies on the ability to program the in vivo retention time, which necessitates real-time monitoring of erosion. However, in vivo performance cannot always be predicted by traditional determination of in vitro erosion1,2, and standard methods sacrifice samples or animals3, preventing sequential measures of the same specimen. We harnessed non-invasive fluorescence imaging to sequentially follow in vivo material-mass loss to model the degradation of materials hydrolytically (PEG:dextran hydrogel) and enzymatically (collagen). Hydrogel erosion rates in vivo and in vitro correlated, enabling the prediction of in vivo erosion of new material formulations from in vitro data. Collagen in vivo erosion was used to infer physiologic in vitro conditions that mimic erosive in vivo environments. This approach enables rapid in vitro screening of materials, and can be extended to simultaneously determine drug release and material erosion from a drug-eluting scaffold, or cell viability and material fate in tissue-engineering formulations.
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