Imperial College London
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Dr Nuria Oliva-Jorge

Faculty of Engineering, Department of Bioengineering

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 7313n.oliva-jorge Website

 
 
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Location

 

U301ASir Michael Uren HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Duran:2021:10.1021/acsbiomaterials.1c00159,
author = {Duran, Mota JA and Quintanas, Yani J and Almquist, B and Borros, S and Oliva, Jorge N},
doi = {10.1021/acsbiomaterials.1c00159},
journal = {ACS Biomaterials Science and Engineering},
pages = {4347--4361},
title = {Polyplex-loaded hydrogels for local gene delivery to human dermal fibroblasts},
url = {http://dx.doi.org/10.1021/acsbiomaterials.1c00159},
volume = {7},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Impaired cutaneous healing leading to chronic wounds affects between 2 and 6% of the total population in most developed countries and it places a substantial burden on healthcare budgets. Current treatments involving antibiotic dressings and mechanical debridement are often not effective, causing severe pain, emotional distress, and social isolation in patients for years or even decades, ultimately resulting in limb amputation. Alternatively, gene therapy (such as mRNA therapies) has emerged as a viable option to promote wound healing through modulation of gene expression. However, protecting the genetic cargo from degradation and efficient transfection into primary cells remain significant challenges in the push to clinical translation. Another limiting aspect of current therapies is the lack of sustained release of drugs to match the therapeutic window. Herein, we have developed an injectable, biodegradable and cytocompatible hydrogel-based wound dressing that delivers poly(β-amino ester)s (pBAEs) nanoparticles in a sustained manner over a range of therapeutic windows. We also demonstrate that pBAE nanoparticles, successfully used in previous in vivo studies, protect the mRNA load and efficiently transfect human dermal fibroblasts upon sustained release from the hydrogel wound dressing. This prototype wound dressing technology can enable the development of novel gene therapies for the treatment of chronic wounds.
AU  - Duran,Mota JA
AU  - Quintanas,Yani J
AU  - Almquist,B
AU  - Borros,S
AU  - Oliva,Jorge N
DO  - 10.1021/acsbiomaterials.1c00159
EP  - 4361
PY  - 2021///
SN  - 2373-9878
SP  - 4347
TI  - Polyplex-loaded hydrogels for local gene delivery to human dermal fibroblasts
T2  - ACS Biomaterials Science and Engineering
UR  - http://dx.doi.org/10.1021/acsbiomaterials.1c00159
UR  - https://pubs.acs.org/doi/10.1021/acsbiomaterials.1c00159
UR  - http://hdl.handle.net/10044/1/89817
VL  - 7
ER  -
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