Imperial College London
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DrNathanSkene

Faculty of Medicine, Department of Brain Sciences

Lecturer in Dementia Research, UK DRI Group Leader
 
 
 
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Contact

 

n.skene Website

 
 
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Location

 

515Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Munoz-Manchado:2018:10.1016/j.celrep.2018.07.053,
author = {Munoz-Manchado, AB and Gonzales, CB and Zeisel, A and Munguba, H and Bekkouche, B and Skene, NG and Lonnerberg, P and Ryge, J and Harris, KD and Linnarsson, S and Hjerling-Leffier, J},
doi = {10.1016/j.celrep.2018.07.053},
journal = {Cell Reports},
pages = {2179--2190.e1--e7},
title = {Diversity of interneurons in the dorsal striatum revealed by single-cell RNA sequencing and PatchSeq},
url = {http://dx.doi.org/10.1016/j.celrep.2018.07.053},
volume = {24},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Striatal locally projecting neurons, or interneurons, act on nearby circuits and shape functional output to the rest of the basal ganglia. We performed single-cell RNA sequencing of striatal cells enriching for interneurons. We find seven discrete interneuron types, six of which are GABAergic. In addition to providing specific markers for the populations previously described, including those expressing Sst/Npy, Th, Npy without Sst, and Chat, we identify two small populations of cells expressing Cck with or without Vip. Surprisingly, the Pvalb-expressing cells do not constitute a discrete cluster but rather are part of a larger group of cells expressing Pthlh with a spatial gradient of Pvalb expression. Using PatchSeq, we show that Pthlh cells exhibit a continuum of electrophysiological properties correlated with expression of Pvalb. Furthermore, we find significant molecular differences that correlate with differences in electrophysiological properties between Pvalb-expressing cells of the striatum and those of the cortex.
AU  - Munoz-Manchado,AB
AU  - Gonzales,CB
AU  - Zeisel,A
AU  - Munguba,H
AU  - Bekkouche,B
AU  - Skene,NG
AU  - Lonnerberg,P
AU  - Ryge,J
AU  - Harris,KD
AU  - Linnarsson,S
AU  - Hjerling-Leffier,J
DO  - 10.1016/j.celrep.2018.07.053
EP  - 2190
PY  - 2018///
SN  - 2211-1247
SP  - 2179
TI  - Diversity of interneurons in the dorsal striatum revealed by single-cell RNA sequencing and PatchSeq
T2  - Cell Reports
UR  - http://dx.doi.org/10.1016/j.celrep.2018.07.053
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000442693600023&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/67296
VL  - 24
ER  -
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