Imperial College London
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DrNathanSkene

Faculty of Medicine, Department of Brain Sciences

Lecturer in Dementia Research, UK DRI Group Leader
 
 
 
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Contact

 

n.skene Website

 
 
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Location

 

515Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Thrupp:2020:10.1016/j.celrep.2020.108189,
author = {Thrupp, N and Frigerio, CS and Wolfs, L and Skene, NG and Fattorelli, N and Poovathingal, S and Fourne, Y and Matthews, PM and Theys, T and Mancuso, R and de, Strooper B and Fiers, M},
doi = {10.1016/j.celrep.2020.108189},
journal = {Cell Reports},
pages = {1--13},
title = {Single-nucleus RNA-seq is not suitable for detection of microglial activation genes in humans},
url = {http://dx.doi.org/10.1016/j.celrep.2020.108189},
volume = {32},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Single-nucleus RNA sequencing (snRNA-seq) is used as an alternative to single-cell RNA-seq, as it allows transcriptomic profiling of frozen tissue. However, it is unclear whether snRNA-seq is able to detect cellular state in human tissue. Indeed, snRNA-seq analyses of human brain samples have failed to detect a consistent microglial activation signature in Alzheimer’s disease. Our comparison of microglia from single cells and single nuclei of four human subjects reveals that, although most genes show similar relative abundances in cells and nuclei, a small population of genes (∼1%) is depleted in nuclei compared to whole cells. This population is enriched for genes previously implicated in microglial activation, including APOE, CST3, SPP1, and CD74, comprising 18% of previously identified microglial-disease-associated genes. Given the low sensitivity of snRNA-seq to detect many activation genes, we conclude that snRNA-seq is not suited for detecting cellular activation in microglia in human disease.
AU  - Thrupp,N
AU  - Frigerio,CS
AU  - Wolfs,L
AU  - Skene,NG
AU  - Fattorelli,N
AU  - Poovathingal,S
AU  - Fourne,Y
AU  - Matthews,PM
AU  - Theys,T
AU  - Mancuso,R
AU  - de,Strooper B
AU  - Fiers,M
DO  - 10.1016/j.celrep.2020.108189
EP  - 13
PY  - 2020///
SN  - 2211-1247
SP  - 1
TI  - Single-nucleus RNA-seq is not suitable for detection of microglial activation genes in humans
T2  - Cell Reports
UR  - http://dx.doi.org/10.1016/j.celrep.2020.108189
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000573722100004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.sciencedirect.com/science/article/pii/S2211124720311785?via%3Dihub
UR  - http://hdl.handle.net/10044/1/84585
VL  - 32
ER  -
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