Imperial College London

DrNeloferSyed

Faculty of MedicineDepartment of Medicine

Senior Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 5292n.syed

 
 
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Location

 

E506Burlington DanesHammersmith Campus

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Summary

 

Publications

Publication Type
Year
to

103 results found

Hatzimichael E, Syed N, Lo Nigro C, Crook Tet al., 2014, A blood test to identify when melanoma metastasizes: a reality for melanoma management?, Melanoma Manag, Vol: 1, Pages: 11-14

JOURNAL ARTICLE

Hatzimichael E, Syed N, Lo Nigro C, Rao B, Crook Tet al., 2014, How detection of epigenetic alterations of blood-borne DNA could improve melanoma diagnosis, EXPERT REVIEW OF MOLECULAR DIAGNOSTICS, Vol: 14, Pages: 639-642, ISSN: 1473-7159

JOURNAL ARTICLE

Allen MD, Phuong L, Hudson C, Leyton J, Delage B, Ghazaly E, Cutts R, Yuan M, Syed N, Lo Nigro C, Lattanzio L, Chmielewska-Kassassir M, Tomlinson I, Roylance R, Whitaker HC, Warren AY, Neal D, Frezza C, Beltran L, Jones LJ, Chelala C, Wu B-W, Bomalaski JS, Jackson RC, Lu Y-J, Crook T, Lemoine NR, Mather S, Foster J, Sosabowski J, Avril N, Li C-F, Szlosarek PWet al., 2014, Prognostic and Therapeutic Impact of Argininosuccinate Synthetase 1 Control in Bladder Cancer as Monitored Longitudinally by PET Imaging, CANCER RESEARCH, Vol: 74, Pages: 896-907, ISSN: 0008-5472

JOURNAL ARTICLE

Asavarut P, O'Neill K, Syed N, Hajitou Aet al., 2014, Chimeric adeno-associated virus and bacteriophage: a potential targeted gene therapy vector for malignant glioma., Ther Deliv, Vol: 5, Pages: 975-990

The incipient development of gene therapy for cancer has fuelled its progression from bench to bedside in mere decades. Of all malignancies that exist, gliomas are the largest class of brain tumors, and are renowned for their aggressiveness and resistance to therapy. In order for gene therapy to achieve clinical success, a multitude of barriers ranging from glioma tumor physiology to vector biology must be overcome. Many viral gene delivery systems have been subjected to clinical investigation; however, with highly limited success. In this review, the current progress and challenges of gene therapy for malignant glioma are discussed. Moreover, we highlight the hybrid adeno-associated virus and bacteriophage vector as a potential candidate for targeted gene delivery to brain tumors.

JOURNAL ARTICLE

Lo Nigro C, Wang H, McHugh A, Lattanzio L, Matin R, Harwood C, Syed N, Hatzimichael E, Briasoulis E, Merlano M, Evans A, Thompson A, Leigh I, Fleming C, Inman GJ, Proby C, Crook Tet al., 2013, Methylated Tissue Factor Pathway Inhibitor 2 (TFP12) DNA in Serum Is a Biomarker of Metastatic Melanoma, JOURNAL OF INVESTIGATIVE DERMATOLOGY, Vol: 133, Pages: 1278-1285, ISSN: 0022-202X

JOURNAL ARTICLE

Przystal JM, Umukoro E, Stoneham CA, Yata T, O'Neill K, Syed N, Hajitou Aet al., 2013, Proteasome inhibition in cancer is associated with enhanced tumor targeting by the adeno-associated virus/phage, MOLECULAR ONCOLOGY, Vol: 7, Pages: 55-66, ISSN: 1574-7891

JOURNAL ARTICLE

Syed N, Langer J, Janczar K, Singh P, Lo Nigro C, Lattanzio L, Coley HM, Hatzimichael E, Bomalaski J, Szlosarek P, Awad M, O'Neil K, Roncaroli F, Crook Tet al., 2013, Epigenetic status of argininosuccinate synthetase and argininosuccinate lyase modulates autophagy and cell death in glioblastoma, CELL DEATH & DISEASE, Vol: 4, ISSN: 2041-4889

JOURNAL ARTICLE

Hatzimichael E, Lo Nigro C, Lattanzio L, Syed N, Shah R, Dasoula A, Janczar K, Vivenza D, Monteverde M, Merlano M, Papoudou-Bai A, Bai M, Schmid P, Stebbing J, Bower M, Dyer MJS, Karran LE, ElguetaKarstegl C, Farrell PJ, Thompson A, Briasoulis E, Crook Tet al., 2012, The collagen prolyl hydroxylases are novel transcriptionally silenced genes in lymphoma, BRITISH JOURNAL OF CANCER, Vol: 107, Pages: 1423-1432, ISSN: 0007-0920

JOURNAL ARTICLE

Cavicchioli F, Shia A, O'Leary K, Haley V, Palmieri C, Syed N, Crook T, Thompson AM, Lo Nigro C, Schmid Pet al., 2012, EPIGENETIC SILENCING OF ARGININO-SUCCINATE SYNTHASE (ASS1) DEFINES ARGININE DEPLETION THERAPY AS A NOVEL TREATMENT STRATEGY FOR BREAST CANCER, 37th Congress of the European-Society-for-Medical-Oncology (ESMO), Publisher: OXFORD UNIV PRESS, Pages: 532-533, ISSN: 0923-7534

CONFERENCE PAPER

Syed N, Roncaroli F, Janczar K, Singh P, O'Neil K, Lo Nigro C, Lattanzio L, Coley HM, Hatzimichael E, Bomalaski J, Szlosarek P, Crook Tet al., 2012, TRANSCRIPTIONAL SILENCING DISRUPTS TWO LEVELS OF ARGININE BIOSYNTHESIS IN GLIOBLASTOMA MULTIFORME (GBM): A NOVEL, TARGETED THERAPEUTIC STRATEGY FOR GBM, Conference of the British-Neuro-Oncology-Society (BNOS), Publisher: OXFORD UNIV PRESS INC, Pages: 11-11, ISSN: 1522-8517

CONFERENCE PAPER

Palmieri C, Monteverde M, Lattanzio L, Gojis O, Rudraraju B, Fortunato M, Syed N, Thompson A, Garrone O, Merlano M, Lo Nigro C, Crook Tet al., 2012, Site-specific CpG methylation in the CCAAT/enhancer binding protein delta (CEBP delta) CpG island in breast cancer is associated with metastatic relapse, BRITISH JOURNAL OF CANCER, Vol: 107, Pages: 732-738, ISSN: 0007-0920

JOURNAL ARTICLE

Palmieri C, Rudraraju B, Monteverde M, Lattanzio L, Gojis O, Brizio R, Garrone O, Merlano M, Syed N, Lo Nigro C, Crook Tet al., 2012, Methylation of the calcium channel regulatory subunit alpha 2 delta-3 (CACNA2D3) predicts site-specific relapse in oestrogen receptor-positive primary breast carcinomas, BRITISH JOURNAL OF CANCER, Vol: 107, Pages: 375-381, ISSN: 0007-0920

JOURNAL ARTICLE

Vivenza D, Gasco M, Monteverde M, Lattanzio L, Syed N, Colantonio I, Denaro N, Natoli G, Comino A, Russi E, Merlano M, Crook T, Lo Nigro Cet al., 2012, MDM2 309 polymorphism predicts outcome in platinum-treated locally advanced head and neck cancer, ORAL ONCOLOGY, Vol: 48, Pages: 602-607, ISSN: 1368-8375

JOURNAL ARTICLE

Delage B, Luong P, Maharaj L, O'Riain C, Syed N, Crook T, Hatzimichael E, Papoudou-Bai A, Mitchell TJ, Whittaker SJ, Cerio R, Gribben J, Lemoine N, Bomalaski J, Li C-F, Joel S, Fitzgibbon J, Chen L-T, Szlosarek PWet al., 2012, Promoter methylation of argininosuccinate synthetase-1 sensitises lymphomas to arginine deiminase treatment, autophagy and caspase-dependent apoptosis, CELL DEATH & DISEASE, Vol: 3, ISSN: 2041-4889

JOURNAL ARTICLE

Lo Nigro C, Monteverde M, Lee S, Lattanzio L, Vivenza D, Comino A, Syed N, McHugh A, Wang H, Proby C, Garrone O, Merlano M, Hatzimichael E, Briasoulis E, Gojis O, Palmieri C, Jordan L, Quinlan P, Thompson A, Crook Tet al., 2012, NT5E CpG island methylation is a favourable breast cancer biomarker, BRITISH JOURNAL OF CANCER, Vol: 107, Pages: 75-83, ISSN: 0007-0920

JOURNAL ARTICLE

Hatzimichael E, Dasoula A, Syed N, Szlosarek PW, Dranitsaris G, Crook T, Briasoulis ECet al., 2012, Epigenetic inactivation to target the arginine biosynthetic pathway in multiple myeloma., 48th Annual Meeting of the American-Society-of-Clinical-Oncology (ASCO), Publisher: AMER SOC CLINICAL ONCOLOGY, ISSN: 0732-183X

CONFERENCE PAPER

Coley HM, Chivers P, Syed N, Crook Tet al., 2012, Methylated PLK2 predicts response to taxanes, CANCER RESEARCH, Vol: 72, ISSN: 0008-5472

JOURNAL ARTICLE

Safuwan NAM, Chivers P, Crook T, Syed N, Coley HMet al., 2012, Epigenetics, cell cycle and drug-resistant ovarian cancer, CANCER RESEARCH, Vol: 72, ISSN: 0008-5472

JOURNAL ARTICLE

Lo Nigro C, Monteverde M, Lee S, Lattanzio L, Syed N, Garrone O, Merlano M, Hatzimichael E, Briasoulis E, Thompson A, Crook Tet al., 2012, NT5E promoter methylation is a favorable breast cancer epigenetic biomarker, CANCER RESEARCH, Vol: 72, ISSN: 0008-5472

JOURNAL ARTICLE

Wang H, Lee S, Lo Nigro C, Lattanzio L, Merlano M, Monteverde M, Matin R, Purdie K, Mladkova N, Bergamaschi D, Harwood C, Syed N, Szlosarek P, Briasoulis E, McHugh A, Thompson A, Evans A, Leigh I, Fleming C, Inman GJ, Hatzimichael E, Proby C, Crook Tet al., 2012, NT5E (CD73) is epigenetically regulated in malignant melanoma and associated with metastatic site specificity, BRITISH JOURNAL OF CANCER, Vol: 106, Pages: 1446-1452, ISSN: 0007-0920

JOURNAL ARTICLE

Lo Nigro C, Vivenza D, Monteverde M, Lattanzio L, Gojis O, Garrone O, Comino A, Merlano M, Quinlan PR, Syed N, Purdie CA, Thompson A, Palmieri C, Crook Tet al., 2012, High frequency of complex TP53 mutations in CNS metastases from breast cancer, BRITISH JOURNAL OF CANCER, Vol: 106, Pages: 397-404, ISSN: 0007-0920

JOURNAL ARTICLE

Coley HM, Hatzimichael E, Blagden S, McNeish I, Thompson A, Crook T, Syed Net al., 2012, Polo Like Kinase 2 Tumour Suppressor and cancer biomarker: new perspectives on drug sensitivity/resistance in cancer, ONCOTARGET, Vol: 3, Pages: 78-83, ISSN: 1949-2553

JOURNAL ARTICLE

Lo Nigro C, Syed N, Lattanzio L, Dasoula A, Papoudou-Bai A, Schmid P, Merlano M, Hatzimichael E, Crook Tet al., 2011, The Prolyl-3-hydroxylases (P3H) and P3H-related Genes CRTAP and SC65 Are Novel Transcriptionally Silenced Genes in Burkitt's Lymphoma, European Multidisciplinary Cancer Congress on Integrating Basic and Translational Science, Surgery, Radiotherapy, Medical oncology, Advocacy and Care, Publisher: ELSEVIER SCI LTD, Pages: S107-S107, ISSN: 0959-8049

CONFERENCE PAPER

Benetatos L, Dasoula A, Hatzimichael E, Syed N, Voukelatou M, Dranitsaris G, Bourantas KL, Crook Tet al., 2011, Polo-like kinase 2 (SNK/PLK2) is a novel epigenetically regulated gene in acute myeloid leukemia and myelodysplastic syndromes: genetic and epigenetic interactions, ANNALS OF HEMATOLOGY, Vol: 90, Pages: 1037-1045, ISSN: 0939-5555

JOURNAL ARTICLE

Gasco M, Vivenza D, Monteverde M, Lattanzio L, Colantonio I, Natoli G, Miraglio E, Comino A, Syed N, Crook T, Merlano MC, Lo Nigro Cet al., 2011, MDM2 309 single nucleotide polymorphisms (SNP) and clinical outcome in patients with advanced squamous cell carcinoma of head and neck (SCCHN)., JOURNAL OF CLINICAL ONCOLOGY, Vol: 29, ISSN: 0732-183X

JOURNAL ARTICLE

Lo Nigro C, Lattanzio L, Monteverde M, Syed N, Thompson AM, Garrone O, Cavicchioli F, Tonissi F, Comino A, McHugh A, Roncaroli F, Palmieri C, Schmid P, Merlano MC, Crook Tet al., 2011, The frequency of methylation of the NT5E gene in metastatic breast cancer., J Clin Oncol, Vol: 29

e11553 Background: Ecto-5-prime-nucleotidase (NT5E; CD73) catalyzes the conversion of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. Deficiency of NT5 occurs in a variety of immunodeficiency diseases and some studies have associated over-expression of NT5E with clinically aggressive neoplastic disease. Here we describe transcriptional down-regulation of the NT5E gene in breast cancer and show that this is associated with aberrant methylation in the CpG island located in its 5' regulatory sequences. METHODS: Methylation in the NT5E CpG island was analysed by methylation specific PCR (MSP) and quantitative pyrosequencing in a panel of 10 breast carcinoma cell lines and two independent clinical series of breast carcinomas, comprising 80 and 140 cases respectively and 17 brain metastatic breast cancer lesions. The breast carcinomas were predominantly invasive ductal carcinomas, untreated at the time of surgery and randomly selected from our clinical practice. Expression was assessed by qRT-PCR. RESULTS: NT5E mRNA was down-regulated in 4/10 breast carcinoma cell lines analysed. Down-regulation was associated with aberrant methylation in the CpG island located in the 5' regulatory sequences of the NT5E gene: methylation was present only in cell lines with down-regulation and the CpG island was unmethylated in cell lines which express the gene. Moreover, expression of NT5E was up-regulated by azacytidine. In clinical cases, methylation (assessed by pyrosequencing and/or MSP) was present in 28% and 41% of cases in independent clinical series of primary carcinomas. Methylation was more common in primary cases which ultimately relapsed at distant metastatic sites. Strikingly, methylation was particularly common in 9/16 brain metastatic lesions. CONCLUSIONS: In contrast to previous reports, we show that methylation of NT5E is common in metastatic breast cancer, particularly in intra-cranial metastases. Our data implicate transcriptional silen

JOURNAL ARTICLE

Gasco M, Vivenza D, Monteverde M, Lattanzio L, Colantonio I, Natoli G, Miraglio E, Comino A, Syed N, Crook T, Merlano MC, Lo Nigro Cet al., 2011, MDM2 309 single nucleotide polymorphisms (SNP) and clinical outcome in patients with advanced squamous cell carcinoma of head and neck (SCCHN)., J Clin Oncol, Vol: 29

5509 Background: Disruptive TP53 alterations have been associated with decreased survival in patients with SCCHN. A T>G polymorphism in the promoter region of mouse double minute 2 homologue (MDM2) has been associated with higher MDM2mRNA and protein levels. Overexpression of MDM2 is thought to interfere with p53-mediated apoptosis and growth inhibition. We hypothesized that the MDM2genotypes containing the G allele (GT+ GGgenotype) may be associated with worse survival in SCCHN patients (pts). METHODS: We directly sequenced the MDM2 polymorphism on both strands. We then evaluated the correlations between MDM2 polymorphism status and overall survival (OS) in 58 pts with stage III and IV SCCHN. Median follow-up was 6.5 years. All pts received cisplatin-based chemo-radiotherapy in our Department from 1997 to 2007. The Kaplan-Meier method and log-rank test were used to compare survival according to MDM2polymorphism status. Cox proportional hazards model was used to calculate hazard ratio (HR) adjusted for possible confounding variables, with 95% confidence interval (95% CI). RESULTS: Median age was 56 years; 50 pts (86%) were male and 52 pts (90%) were stage IV. The genotype frequencies for the MDM2 polymorphism were: T/T 32 (55%), T/G 17 (30%), G/G 9 (15%). Median overall survival (OS) and 5-years survival rates were significantly shorter in patients with genotypes containing the G allele (GT + GG genotype) compared to those with TT genotype (63 months vs. 124 months; log- rank p = 0.006 and 10/26 vs. 27/32; Fisher's exact p = 0.02, respectively). Notably, after adjusting for age, stage and performance status, genotypes containing the G allele appeared to be associated with worse survival (HR for death 2.90; 95% CI 1.37-6.12; p = 0.005). CONCLUSIONS: Our findings support the hypothesis that MDM2SNP309 polymorphism is an independent survival determinant among advanced stage SCCHN patients treated with cisplatin-based chemo-radiotherapy.

JOURNAL ARTICLE

Lo Nigro C, Lattanzio L, Monteverde M, Syed N, Thompson AM, Garrone O, Cavicchioli F, Tonissi F, Comino A, McHugh A, Roncaroli F, Palmieri C, Schmid P, Merlano MC, Crook Tet al., 2011, The frequency of methylation of the NT5E gene in metastatic breast cancer, JOURNAL OF CLINICAL ONCOLOGY, Vol: 29, ISSN: 0732-183X

JOURNAL ARTICLE

Syed N, Coley HM, Sehouli J, Koensgen D, Mustea A, Szlosarek P, McNeish I, Blagden SP, Schmid P, Lovell DP, Hatzimichael E, Crook Tet al., 2011, Polo-like Kinase Plk2 Is an Epigenetic Determinant of Chemosensitivity and Clinical Outcomes in Ovarian Cancer, CANCER RESEARCH, Vol: 71, Pages: 3317-3327, ISSN: 0008-5472

JOURNAL ARTICLE

Syed N, O'Neilli K, Hughes M, LoNigro C, Lattanzio L, Szlosarek P, Roncaroli F, Crook Tet al., 2011, Transcriptional silencing disrupts two levels of arginine biosynthesis in glioblastoma multiforme: a novel, targeted therapeutic strategy for high grade gliomas, CANCER RESEARCH, Vol: 71, ISSN: 0008-5472

JOURNAL ARTICLE

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