Imperial College London

DrNeloferSyed

Faculty of MedicineDepartment of Medicine

Senior Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 5292n.syed

 
 
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Location

 

E506Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Atkinson:2018:10.1016/j.jid.2018.10.038,
author = {Atkinson, A and Renziehausen, A and Wang, H and Lo, Nigro C and Lattanzio, L and Merlano, M and Rao, B and Weir, L and Evans, A and Matin, R and Harwood, C and Szlosarek, P and Pickering, JG and Fleming, C and Sim, VR and Li, S and Vasta, JT and Raines, RT and Boniol, M and Thompson, A and Proby, C and Crook, T and Syed, N},
doi = {10.1016/j.jid.2018.10.038},
journal = {J Invest Dermatol},
title = {Collagen Prolyl Hydroxylases Are Bifunctional Growth Regulators in Melanoma.},
url = {http://dx.doi.org/10.1016/j.jid.2018.10.038},
year = {2018}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Appropriate post-translational processing of collagen requires prolyl hydroxylation, catalyzed by collagen prolyl 3-hydroxylase and collagen prolyl 4-hydroxylase, and is essential for normal cell function. Here we have investigated the expression, transcriptional regulation, and function of the collagen prolyl 3-hydroxylase and collagen prolyl 4-hydroxylase families in melanoma. We show that the collagen prolyl 3-hydroxylase family exemplified by Leprel1 and Leprel2 is subject to methylation-dependent transcriptional silencing in primary and metastatic melanoma consistent with a tumor suppressor function. In contrast, although there is transcriptional silencing of P4HA3 in a subset of melanomas, the collagen prolyl 4-hydroxylase family members P4HA1, P4HA2, and P4HA3 are often overexpressed in melanoma, expression being prognostic of worse clinical outcomes. Consistent with tumor suppressor function, ectopic expression of Leprel1 and Leprel2 inhibits melanoma proliferation, whereas P4HA2 and P4HA3 increase proliferation, and particularly invasiveness, of melanoma cells. Pharmacological inhibition with multiple selective collagen prolyl 4-hydroxylase inhibitors reduces proliferation and inhibits invasiveness of melanoma cells. Together, our data identify the collagen prolyl 3-hydroxylase and collagen prolyl 4-hydroxylase families as potentially important regulators of melanoma growth and invasiveness and suggest that selective inhibition of collagen prolyl 4-hydroxylase is an attractive strategy to reduce the invasive properties of melanoma cells.
AU - Atkinson,A
AU - Renziehausen,A
AU - Wang,H
AU - Lo,Nigro C
AU - Lattanzio,L
AU - Merlano,M
AU - Rao,B
AU - Weir,L
AU - Evans,A
AU - Matin,R
AU - Harwood,C
AU - Szlosarek,P
AU - Pickering,JG
AU - Fleming,C
AU - Sim,VR
AU - Li,S
AU - Vasta,JT
AU - Raines,RT
AU - Boniol,M
AU - Thompson,A
AU - Proby,C
AU - Crook,T
AU - Syed,N
DO - 10.1016/j.jid.2018.10.038
PY - 2018///
TI - Collagen Prolyl Hydroxylases Are Bifunctional Growth Regulators in Melanoma.
T2 - J Invest Dermatol
UR - http://dx.doi.org/10.1016/j.jid.2018.10.038
UR - https://www.ncbi.nlm.nih.gov/pubmed/30452903
UR - http://hdl.handle.net/10044/1/65729
ER -