Imperial College London

DrNeloferSyed

Faculty of MedicineDepartment of Medicine

Senior Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 5292n.syed

 
 
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Location

 

E506Burlington DanesHammersmith Campus

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Summary

 

Publications

Publication Type
Year
to

99 results found

El Mubarak MA, Stylos EK, Chatziathanasiadou MV, Danika C, Alexiou GA, Tsekeris P, Renziehausen A, Crook T, Syed N, Sivolapenko GB, Tzakos AGet al., 2018, Development and validation of simple step protein precipitation UHPLC-MS/MS methods for quantitation of temozolomide in cancer patient plasma samples., J Pharm Biomed Anal, Vol: 162, Pages: 164-170

Temozolomide (TEMODAL™) (TMZ) is an antineoplastic agent that is primarily used for the treatment of glioblastoma and anaplastic gliomas, two aggressive forms of brain cancer. Due to the poor prognosis of brain tumour patients, there is an increasing body of research into improving the stability and delivery of TMZ past the blood brain barrier using carrier molecules. These require accurate determination of TMZ levels for biodistribution and pharmacokinetic evaluation. Unfortunately, current methodologies for the determination of TMZ in human plasma suffer from low reproducibility, recovery, sensitivity or cost ineffective procedures associated with extensive sample cleaning. To surpass these disadvantages, we developed two bioanalytical methods with high sensitivity and excellent recovery for the determination of TMZ in human plasma at minimum cost. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was used and both methods were validated under US Food and Drug Administration (FDA) and the European Medicines Agency (EMEA) guidelines. The two methods had minor differences in the sample pre-treatment and each method was developed and applied in separate laboratories. Theophylline was selected as internal standard (IS). Calibration curves were linear over the range of 10-500 ng/mL with extraction recovery ranging from 77.3 to 97.3% while all validation parameters met the acceptance criteria and proved the methods' reliability. The validated methods were successfully applied to plasma samples donated from cancer patient following treatment with temozolomide.

JOURNAL ARTICLE

Hajji N, García-Domínguez DJ, Hontecillas-Prieto L, O'Neill K, de Álava E, Syed Net al., 2018, The bitter side of epigenetics: variability and resistance to chemotherapy., Epigenomics

One of the major obstacles to the development of effective new cancer treatments and the main factor for the increasing number of clinical trial failures appears to be the paucity of accurate, reproducible and robust drug resistance testing methods. Most research assessing the resistance of cancers to chemotherapy has concentrated on genetic-based molecular mechanisms, while the role of epigenetics in drug resistance has been generally overlooked. This is rather surprising given that an increasing body of evidence pointing to the fact that epigenetic mechanism alterations appear to play a pivotal role in cancer initiation, progression and development of chemoresistance. This resulted in a series of clinical trials involving epi-drug as single treatment or combined with cancer conventional drugs. In this review, we provided the main mechanisms by which the epigenetic regulators control the resistance to cancer drugs.

JOURNAL ARTICLE

Lo Nigro C, Renziehausen A, Tzakos AG, Wang H, Rao B, Matin R, Harwood C, Vivenza D, Tonissi F, Occelli M, Weir L, Li S, Sim VR, O'Neill K, Evans A, Thompson A, Szlosarek P, Fleming C, Proby C, Syed N, Merlano M, Crook Tet al., 2018, The renin angiotensin system (RAS) mediates bifunctional growth regulation in melanoma and is a target for therapeutic manipulation, Publisher: BIOMED CENTRAL LTD, ISSN: 1479-5876

CONFERENCE PAPER

Moren L, Perryman R, Crook T, Langer JK, Oneill K, Syed N, Antti Het al., 2018, Metabolomic profiling identifies distinct phenotypes for ASS1 positive and negative GBM, BMC CANCER, Vol: 18, ISSN: 1471-2407

JOURNAL ARTICLE

Moren L, Perryman R, Crook T, Langer JK, Oneill K, Syed N, Antti Het al., 2018, Metabolomic profiling identifies distinct phenotypes for ASS1 positive and negative GBM (vol 18, 167, 2018), BMC CANCER, Vol: 18, ISSN: 1471-2407

JOURNAL ARTICLE

Pankratova S, Klingelhofer J, Dmytriyeva O, Owczarek S, Renziehausen A, Syed N, Porter AE, Dexter DT, Kiryushko Det al., 2018, The S100A4 Protein Signals through the ErbB4 Receptor to Promote Neuronal Survival, THERANOSTICS, Vol: 8, Pages: 3977-3990, ISSN: 1838-7640

JOURNAL ARTICLE

Williams M, Cross H, Jenkinson MD, Martin K, Wood S, Scheck AC, Syed N, O'Neill K, Sheen K, Zabilowicz C, Breen K, Oliver K, Williams E, Johnson M, Fulcher Wet al., 2018, THE KETOGENIC DIET FOR PATIENTS WITH BRAIN TUMOURS: TWO PARALLEL RANDOMISED TRIALS, Meeting of the British-Neuro-Oncology-Society (BNOS), Publisher: OXFORD UNIV PRESS INC, Pages: 7-8, ISSN: 1522-8517

CONFERENCE PAPER

Bassiri K, Ferluga S, Sharma V, Syed N, Adams CL, Lasonder E, Hanemann COet al., 2017, Global Proteome and Phospho-proteome Analysis of Merlin-deficient Meningioma and Schwannoma Identifies PDLIM2 as a Novel Therapeutic Target, EBIOMEDICINE, Vol: 16, Pages: 76-86, ISSN: 2352-3964

JOURNAL ARTICLE

Preston J, Stylianou J, Zeng Q, Glover S, Scheck AC, Woolf EC, O'Neill K, Syed Net al., 2017, THE KETOGENIC DIET INDUCES EPIGENETIC CHANGES THAT PLAY KEY ROLES IN TUMOUR DEVELOPMENT, Meeting of the British-Neuro-Oncology-Society (BNOS), Publisher: OXFORD UNIV PRESS INC, Pages: 128-128, ISSN: 1522-8517

CONFERENCE PAPER

Tzouliana S, Hajji N, Perryman R, O'Neill K, Woolf EC, Scheck AC, Syed Net al., 2017, THE KETOGENIC DIET ALTERS THE EPIGENETIC LANDSCAPE OF GBM TO POTENTIATE THE EFFECTS OF CHEMO AND RADIOTHERAPY, Joint Conference of 22nd Annual Scientific Meeting and Education Day of the Society-for-Neuro-Oncology / Conference of the Society-for-CNS-Interstitial-Delivery-of-the-Therapeutics (SCIDOT) on Therapeutic Delivery to the CNS, Publisher: OXFORD UNIV PRESS INC, Pages: 96-97, ISSN: 1522-8517

CONFERENCE PAPER

Woolf EC, Bhattacharya A, Rossi AP, Davidson MM, Syed N, Scheck ACet al., 2017, THE KETONE BODY beta-HYDROXYBUTYRATE CHEMO- AND RADIO-SENSITIZES MALIGNANT GLIOMA CELLS BY INHIBITING HISTONE DEACETYLASE ACTIVITY AND DOWNREGULATING EXPRESSION OF RAD51, Joint Conference of 22nd Annual Scientific Meeting and Education Day of the Society-for-Neuro-Oncology / Conference of the Society-for-CNS-Interstitial-Delivery-of-the-Therapeutics (SCIDOT) on Therapeutic Delivery to the CNS, Publisher: OXFORD UNIV PRESS INC, Pages: 83-83, ISSN: 1522-8517

CONFERENCE PAPER

Lo Nigro C, Lattanzio L, Wang H, Proby C, Syed N, Occelli M, Cauchi C, Merlano M, Harwood C, Thompson A, Crook Tet al., 2016, Prolyl 4-(C-P4H) hydroxylases have opposing effects in malignant melanoma: implication in prognosis and therapy, Publisher: BMC, ISSN: 1479-5876

CONFERENCE PAPER

Nevedomskaya E, Perryman R, Solanki S, Syed N, Mayboroda OA, Keun HCet al., 2016, A Systems Oncology Approach Identifies NT5E as a Key Metabolic Regulator in Tumor Cells and Modulator of Platinum Sensitivity, JOURNAL OF PROTEOME RESEARCH, Vol: 15, Pages: 280-290, ISSN: 1535-3893

JOURNAL ARTICLE

Perryman R, O'Neill K, Keun H, Syed Net al., 2016, DETERMINING THE ROLE OF NICOTINAMIDE METABOLISM IN CHEMOSENSITIVITY IN GLIOBLASTOMA MULTIFORME, 21st Annual Scientific Meeting and Education Day of the Society-for-Neuro-Oncology, Publisher: OXFORD UNIV PRESS INC, Pages: 36-36, ISSN: 1522-8517

CONFERENCE PAPER

Renziehausen A, Eschbacher J, O'Neill K, Tzakos A, Crook T, Syed Net al., 2016, ANGIOTENSIN SIGNALLING IN GBM: AT2R AS A NOVEL THERAPEUTIC TARGET, 21st Annual Scientific Meeting and Education Day of the Society-for-Neuro-Oncology, Publisher: OXFORD UNIV PRESS INC, Pages: 40-40, ISSN: 1522-8517

CONFERENCE PAPER

Woolf EC, Rossi AP, Silva-Nichols HB, Gardner KD, Syed N, Scheck ACet al., 2016, THE KETONE BODY beta-HYDROXYBUTYRATE INHIBITS HISTONE DEACETYLASE ACTIVITY AND ALTERS EXPRESSION OF DNA REPAIR PROTEINS IN MALIGNANT GLIOMA CELLS, 21st Annual Scientific Meeting and Education Day of the Society-for-Neuro-Oncology, Publisher: OXFORD UNIV PRESS INC, Pages: 62-63, ISSN: 1522-8517

CONFERENCE PAPER

Woolf EC, Syed N, Scheck AC, 2016, Tumor Metabolism, the Ketogenic Diet and beta-Hydroxybutyrate: Novel Approaches to Adjuvant Brain Tumor Theraphy, FRONTIERS IN MOLECULAR NEUROSCIENCE, Vol: 9, ISSN: 1662-5099

JOURNAL ARTICLE

Abaitua F, Przystal J, Hajitou A, Syed Net al., 2015, Arginine deprivation using ADI-PEG20 leads to regression of an ASS1-ve intracranial GBM tumor in mice and potentiates gamma irradiation of ASS1+ve GBM in vitro, 106th Annual Meeting of the American-Association-for-Cancer-Research (AACR), Publisher: AMER ASSOC CANCER RESEARCH, ISSN: 0008-5472

CONFERENCE PAPER

Ingemarsdotter CK, Tookman LA, Browne A, Pirlo K, Cutts R, Chelela C, Khurrum KF, Leung EYL, Dowson S, Webber L, Khan I, Ennis D, Syed N, Crook TR, Brenton JD, Lockley M, McNeish IAet al., 2015, Paclitaxel resistance increases oncolytic adenovirus efficacy via upregulated CAR expression and dysfunctional cell cycle control, MOLECULAR ONCOLOGY, Vol: 9, Pages: 791-805, ISSN: 1574-7891

JOURNAL ARTICLE

Pazmandi J, O'Neill KS, Scheck AC, Szlosarek PW, Woolf EC, Brooks KS, Syed Net al., 2015, The ketogenic diet alters the expression of microRNAs that play key roles in tumor development, 106th Annual Meeting of the American-Association-for-Cancer-Research (AACR), Publisher: AMER ASSOC CANCER RESEARCH, ISSN: 0008-5472

CONFERENCE PAPER

Yata T, Lee ELQ, Suwan K, Syed N, Asavarut P, Hajitou Aet al., 2015, Modulation of extracellular matrix in cancer is associated with enhanced tumor cell targeting by bacteriophage vectors, MOLECULAR CANCER, Vol: 14, ISSN: 1476-4598

JOURNAL ARTICLE

Abaitua F, Crook T, O'Neill K, Syed Net al., 2014, TARGETING COLLAGEN REGULATION IN GLIOBLASTOMA MULTIFORME, Meeting of the British-Neuro-Oncology-Society (BNOS), Publisher: OXFORD UNIV PRESS INC, ISSN: 1522-8517

CONFERENCE PAPER

Abaitua F, Crook T, O'Neill K, Syed Net al., 2014, TARGETING COLLAGEN REGULATION IN GLIOBLASTOMA MULTIFORME, NEURO-ONCOLOGY, Vol: 16, ISSN: 1522-8517

JOURNAL ARTICLE

Allen MD, Phuong L, Hudson C, Leyton J, Delage B, Ghazaly E, Cutts R, Yuan M, Syed N, Lo Nigro C, Lattanzio L, Chmielewska-Kassassir M, Tomlinson I, Roylance R, Whitaker HC, Warren AY, Neal D, Frezza C, Beltran L, Jones LJ, Chelala C, Wu B-W, Bomalaski JS, Jackson RC, Lu Y-J, Crook T, Lemoine NR, Mather S, Foster J, Sosabowski J, Avril N, Li C-F, Szlosarek PWet al., 2014, Prognostic and Therapeutic Impact of Argininosuccinate Synthetase 1 Control in Bladder Cancer as Monitored Longitudinally by PET Imaging, CANCER RESEARCH, Vol: 74, Pages: 896-907, ISSN: 0008-5472

JOURNAL ARTICLE

Asavarut P, O'Neill K, Syed N, Hajitou Aet al., 2014, Chimeric adeno-associated virus and bacteriophage: a potential targeted gene therapy vector for malignant glioma., Ther Deliv, Vol: 5, Pages: 975-990

The incipient development of gene therapy for cancer has fuelled its progression from bench to bedside in mere decades. Of all malignancies that exist, gliomas are the largest class of brain tumors, and are renowned for their aggressiveness and resistance to therapy. In order for gene therapy to achieve clinical success, a multitude of barriers ranging from glioma tumor physiology to vector biology must be overcome. Many viral gene delivery systems have been subjected to clinical investigation; however, with highly limited success. In this review, the current progress and challenges of gene therapy for malignant glioma are discussed. Moreover, we highlight the hybrid adeno-associated virus and bacteriophage vector as a potential candidate for targeted gene delivery to brain tumors.

JOURNAL ARTICLE

Channathodiyil P, Kardooni H, Khozoie C, Nelofer S, Darling J, Morris M, Warr Tet al., 2014, EPIGENETIC INACTIVATION OF ARGININE BIOSYNTHESIS PATHWAY IN PAEDIATRIC HIGH GRADE GLIOMA, NEURO-ONCOLOGY, Vol: 16, ISSN: 1522-8517

JOURNAL ARTICLE

Hatzimichael E, Syed N, Lo Nigro C, Crook Tet al., 2014, A blood test to identify when melanoma metastasizes: a reality for melanoma management?, Melanoma Manag, Vol: 1, Pages: 11-14

JOURNAL ARTICLE

Hatzimichael E, Syed N, Lo Nigro C, Rao B, Crook Tet al., 2014, How detection of epigenetic alterations of blood-borne DNA could improve melanoma diagnosis, EXPERT REVIEW OF MOLECULAR DIAGNOSTICS, Vol: 14, Pages: 639-642, ISSN: 1473-7159

JOURNAL ARTICLE

Langer J, Elustondo FA, Chan ECY, Antti H, Want E, ONeill K, Syed Net al., 2014, METABOLOMIC ANALYSIS OF GLIOBLASTOMA MULTIFORME UPON ARGININE DEPRIVATION TREATMENT, NEURO-ONCOLOGY, Vol: 16, ISSN: 1522-8517

JOURNAL ARTICLE

Langer JK, Chan ECY, Antti H, Want E, ONeill K, Syed Net al., 2014, METABOLOMIC ANALYSIS OF GLIOBLASTOMA MULTIFORME UPON ARGININE DEPRIVATION TREATMENT, Meeting of the British-Neuro-Oncology-Society (BNOS), Publisher: OXFORD UNIV PRESS INC, ISSN: 1522-8517

CONFERENCE PAPER

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