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@article{Fasulo:2019:10.1101/834630,
author = {Fasulo, B and Meccariello, A and Morgan, M and Borufka, C and Papathanos, PA and Windbichler, N},
doi = {10.1101/834630},
title = {A fly model establishes distinct mechanisms for synthetic CRISPR/Cas9 sex distorters},
url = {http://dx.doi.org/10.1101/834630},
year = {2019}
}

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TY  - JOUR
AB  - <jats:p>Synthetic sex distorters have recently been developed in the malaria mosquito, relying on endonucleases that target the X-chromosome during spermatogenesis. Although inspired by naturally-occurring traits, it has remained unclear how they function and, given their potential for genetic control, how portable this strategy is across species. We established<jats:italic>Drosophila</jats:italic>models for two distinct mechanisms for CRISPR/Cas9 sex-ratio distortion - “X-shredding” and “X-meddling” - and dissected their target-site requirements and repair dynamics. X-shredding relies on sufficient meiotic activity of the endonuclease to overpower DNA repair and can operate on a single repeat cluster of non-essential sequences. X-meddling by contrast, i.e. targeting putative haplolethal X-linked genes, induced a bias towards males that is coupled to a loss in reproductive output, although a dominant-negative effect may drive the mechanism of female lethality. Our model system will guide the study and the application of sex distorters to medically or agriculturally important insect target species.</jats:p>
AU  - Fasulo,B
AU  - Meccariello,A
AU  - Morgan,M
AU  - Borufka,C
AU  - Papathanos,PA
AU  - Windbichler,N
DO  - 10.1101/834630
PY  - 2019///
TI  - A fly model establishes distinct mechanisms for synthetic CRISPR/Cas9 sex distorters
UR  - http://dx.doi.org/10.1101/834630
ER  -

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Nikolai Windbichler

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