Imperial College London
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Dr Nick Powell

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Professor of Gastroenterology
 
 
 
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Contact

 

nicholas.powell

 
 
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Assistant

 

Mrs Heather Bones +44 (0)20 7594 2429

 
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Location

 

Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Alexander:2020:10.14309/ctg.0000000000000296,
author = {Alexander, J and Powell, N},
doi = {10.14309/ctg.0000000000000296},
journal = {Clinical and Translational Gastroenterology},
pages = {e00296--e00296},
title = {Ileocolonic histopathological and microbial alterations in the irritable bowel syndrome: A nested community case-control study},
url = {http://dx.doi.org/10.14309/ctg.0000000000000296},
volume = {12},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - IntroductionHistopathological alterations in the ileum and colon in irritable bowel syndrome (IBS) are controversial, and normal values are poorly established. We hypothesized that changes in mucosal immune cells characterize IBS and key changes in immune composition are associated with the mucosa-associated microbiota (MaM).MethodsA nested case-control study (48 IBS and 106 controls included) from 745 colonoscopy participants in a random population sample. Intraepithelial lymphocytes (IELs)/100 enterocytes and eosinophils/5 nonoverlapping high-power fields counted; mast cells identified by immunocytochemistry (CD117)/5 high-power fields. Paneth cells quantified per 5 crypts. 16S rRNA gene amplicon sequencing performed on available sigmoid MaM, n = 55 and fecal microbiota, n = 20. Microbiota profiles compared between samples with high and low IEL counts.ResultsIBS had increased IELs in the terminal ileum (relative risk ratio = 1.70, 95% confidence interval 1.08-2.76, P = 0.022 adjusted for age, sex, and smoking). Cecal IELs were increased in IBS-diarrhea (relative risk ratio = 2.03, 95% confidence interval 1.13-3.63, P = 0.017). No difference was observed in alpha diversity of MaM or fecal microbiota based on IEL count. There was no difference in beta diversity of the MaM according to IEL count in the terminal ileal (TI) (P = 0.079). High TI IEL counts associated with a significant expansion of the genus Blautia (P = 0.024) and unclassified Clostridiales (P = 0.036) in colon MaM.DiscussionA modest but significant increase in IELs was observed in IBS vs. controls in a population-based setting. Subtle TI and cecal inflammation may play a pathogenic role in IBS but needs confirmation. Modest but discernible differences in the colonic MaM were seen according to TI IEL count but not IBS status.
AU  - Alexander,J
AU  - Powell,N
DO  - 10.14309/ctg.0000000000000296
EP  - 00296
PY  - 2020///
SN  - 2155-384X
SP  - 00296
TI  - Ileocolonic histopathological and microbial alterations in the irritable bowel syndrome: A nested community case-control study
T2  - Clinical and Translational Gastroenterology
UR  - http://dx.doi.org/10.14309/ctg.0000000000000296
UR  - http://hdl.handle.net/10044/1/85920
VL  - 12
ER  -
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