Imperial College London

DrOliverBillker

Faculty of Natural SciencesDepartment of Life Sciences

Honorary Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 5461o.billker

 
 
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Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

53 results found

Sinha A, Hughes KR, Modrzynska KK, Otto TD, Pfander C, Dickens NJ, Religa AA, Bushell E, Graham AL, Cameron R, Kafsack BFC, Williams AE, Llinas M, Berriman M, Billker O, Waters APet al., 2014, A cascade of DNA-binding proteins for sexual commitment and development in Plasmodium, NATURE, Vol: 507, Pages: 253-+, ISSN: 0028-0836

Journal article

Bauza K, Malinauskas T, Pfander C, Anar B, Jones EY, Billker O, Hill AVS, Reyes-Sandoval Aet al., 2014, Efficacy of a Plasmodium vivax Malaria Vaccine Using ChAd63 and Modified Vaccinia Ankara Expressing Thrombospondin-Related Anonymous Protein as Assessed with Transgenic Plasmodium berghei Parasites, INFECTION AND IMMUNITY, Vol: 82, Pages: 1277-1286, ISSN: 0019-9567

Journal article

Brochet M, Collins MO, Smith TK, Thompson E, Sebastian S, Volkmann K, Schwach F, Chappell L, Gomes AR, Berriman M, Rayner JC, Baker DA, Choudhary J, Billker Oet al., 2014, Phosphoinositide Metabolism Links cGMP- Dependent Protein Kinase G to Essential Ca (2+) Signals at Key Decision Points in the Life Cycle of Malaria Parasites, PLOS BIOLOGY, Vol: 12, ISSN: 1544-9173

Journal article

Suarez C, Volkmann K, Gomes AR, Billker O, Blackman MJet al., 2013, The Malarial Serine Protease SUB1 Plays an Essential Role in Parasite Liver Stage Development, PLOS PATHOGENS, Vol: 9, ISSN: 1553-7374

Journal article

Everitt AR, Clare S, McDonald JU, Kane L, Harcourt K, Ahras M, Lall A, Hale C, Rodgers A, Young DB, Haque A, Billker O, Tregoning JS, Dougan G, Kellam Pet al., 2013, Defining the Range of Pathogens Susceptible to Ifitm3 Restriction Using a Knockout Mouse Model, PLOS ONE, Vol: 8, ISSN: 1932-6203

Journal article

Frenal K, Tay CL, Mueller C, Bushell ES, Jia Y, Graindorge A, Billker O, Rayner JC, Soldati-Favre Det al., 2013, Global Analysis of Apicomplexan Protein S-Acyl Transferases Reveals an Enzyme Essential for Invasion, TRAFFIC, Vol: 14, Pages: 895-911, ISSN: 1398-9219

Journal article

Ning J, Otto TD, Pfander C, Schwach F, Brochet M, Bushell E, Goulding D, Sanders M, Lefebvre PA, Pei J, Grishin NV, Vanderlaan G, Billker O, Snell WJet al., 2013, Comparative genomics in Chlamydomonas and Plasmodium identifies an ancient nuclear envelope protein family essential for sexual reproduction in protists, fungi, plants, and vertebrates, GENES & DEVELOPMENT, Vol: 27, Pages: 1198-1215, ISSN: 0890-9369

Journal article

Pfander C, Anar B, Brochet M, Rayner JC, Billker Oet al., 2013, Recombination-mediated genetic engineering of Plasmodium berghei DNA., Methods Mol Biol, Vol: 923, Pages: 127-138

DNA of Plasmodium berghei is difficult to manipulate in Escherichia coli by conventional restriction and ligation methods due to its high content of adenine and thymine (AT) nucleotides. This limits our ability to clone large genes and to generate complex vectors for modifying the parasite genome. We here describe a protocol for using lambda Red recombinase to modify inserts of a P. berghei genomic DNA library constructed in a linear, low-copy, phage-derived vector. The method uses primer extensions of 50 bp, which provide sufficient homology for an antibiotic resistance marker to recombine efficiently with a P. berghei genomic DNA insert in E. coli. In a subsequent in vitro Gateway reaction the bacterial marker is replaced with a cassette for selection in P. berghei. The insert is then released and used for transfection. The basic techniques we describe here can be adapted to generate highly efficient vectors for gene deletion, tagging, targeted mutagenesis, or genetic complementation with larger genomic regions.

Journal article

Pino P, Sebastian S, Kim EA, Bush E, Brochet M, Volkmann K, Kozlowski E, Llinas M, Billker O, Soldati-Favre Det al., 2012, A Tetracycline-Repressible Transactivator System to Study Essential Genes in Malaria Parasites, CELL HOST & MICROBE, Vol: 12, Pages: 824-834, ISSN: 1931-3128

Journal article

Volkmann K, Pfander C, Burstroem C, Ahras M, Goulding D, Rayner JC, Frischknecht F, Billker O, Brochet Met al., 2012, The Alveolin IMC1h Is Required for Normal Ookinete and Sporozoite Motility Behaviour and Host Colonisation in Plasmodium berghei, PLOS ONE, Vol: 7, ISSN: 1932-6203

Journal article

Sebastian S, Brochet M, Collins MO, Schwach F, Jones ML, Goulding D, Rayner JC, Choudhary JS, Billker Oet al., 2012, A Plasmodium Calcium-Dependent Protein Kinase Controls Zygote Development and Transmission by Translationally Activating Repressed mRNAs, CELL HOST & MICROBE, Vol: 12, Pages: 9-19, ISSN: 1931-3128

Journal article

Ojo KK, Pfander C, Mueller NR, Burstroem C, Larson ET, Bryan CM, Fox AMW, Reid MC, Johnson SM, Murphy RC, Kennedy M, Mann H, Leibly DJ, Hewitt SN, Verlinde CLMJ, Kappe S, Merritt EA, Maly DJ, Billker O, Van Voorhis WCet al., 2012, Transmission of malaria to mosquitoes blocked by bumped kinase inhibitors, JOURNAL OF CLINICAL INVESTIGATION, Vol: 122, Pages: 2301-2305, ISSN: 0021-9738

Journal article

Murphy RC, Ojo KK, Billker O, Van Voorhis W, Maly DJet al., 2012, BUMPED KINASE INHIBITORS BLOCK MALARIA TRANSMISSION TO MOSQUITOES, Western Regional Meeting, Publisher: LIPPINCOTT WILLIAMS & WILKINS, Pages: 195-195, ISSN: 1081-5589

Conference paper

Murphy RC, Ojo KK, Billker O, Van Voorhis W, Maly DJet al., 2012, BUMPED KINASE INHIBITORS BLOCK MALARIA TRANSMISSION TO MOSQUITOES, Western Regional Meeting, Publisher: LIPPINCOTT WILLIAMS & WILKINS, Pages: 169-170, ISSN: 1081-5589

Conference paper

Pfander C, Anar B, Schwach F, Otto TD, Brochet M, Volkmann K, Quail MA, Pain A, Rosen B, Skarnes W, Rayner JC, Billker Oet al., 2011, A scalable pipeline for highly effective genetic modification of a malaria parasite, NATURE METHODS, Vol: 8, Pages: 1078-+, ISSN: 1548-7091

Journal article

Raabe A, Berry L, Sollelis L, Cerdan R, Tawk L, Vial HJ, Billker O, Wengelnik Ket al., 2011, Genetic and transcriptional analysis of phosphoinositide-specific phospholipase C in Plasmodium, EXPERIMENTAL PARASITOLOGY, Vol: 129, Pages: 75-80, ISSN: 0014-4894

Journal article

Raabe AC, Wengelnik K, Billker O, Vial HJet al., 2011, Multiple roles for Plasmodium berghei phosphoinositide-specific phospholipase C in regulating gametocyte activation and differentiation, CELLULAR MICROBIOLOGY, Vol: 13, Pages: 955-966, ISSN: 1462-5814

Journal article

Ramos TN, Darley MM, Hu X, Billker O, Rayner JC, Ahras M, Wohler JE, Barnum SRet al., 2011, Cutting Edge: The Membrane Attack Complex of Complement Is Required for the Development of Murine Experimental Cerebral Malaria, JOURNAL OF IMMUNOLOGY, Vol: 186, Pages: 6657-6660, ISSN: 0022-1767

Journal article

Baum J, Billker O, Bousema T, Dinglasan R, McGovern V, Mota MM, Mueller I, Sinden Ret al., 2011, A Research Agenda for Malaria Eradication: Basic Science and Enabling Technologies, PLOS MEDICINE, Vol: 8, ISSN: 1549-1676

Journal article

Tewari R, Straschil U, Bateman A, Boehme U, Cherevach I, Gong P, Pain A, Billker Oet al., 2010, The Systematic Functional Analysis of Plasmodium Protein Kinases Identifies Essential Regulators of Mosquito Transmission, CELL HOST & MICROBE, Vol: 8, Pages: 377-387, ISSN: 1931-3128

Journal article

Doerig C, Billker O, 2010, A parasite calcium switch and Achilles' heel revealed, NATURE STRUCTURAL & MOLECULAR BIOLOGY, Vol: 17, Pages: 541-543, ISSN: 1545-9993

Journal article

Raabe AC, Billker O, Vial HJ, Wengelnik Ket al., 2009, Quantitative assessment of DNA replication to monitor microgametogenesis in Plasmodium berghei, MOLECULAR AND BIOCHEMICAL PARASITOLOGY, Vol: 168, Pages: 172-176, ISSN: 0166-6851

Journal article

Religa AA, Carret C, Feltwell T, Hu G, Amaladoss A, Brochet M, Pain A, Bozdech Z, Preiser PR, Billker O, Janse CJ, Scherf A, Waters APet al., 2009, AN UNEXPECTED ROLE OF SIR2A IN THE LIFE CYCLE OF MALARIA PARASITES, 58th Annual Meeting of the American-Society-of-Tropical-Medicine-and-Hygiene, Publisher: AMER SOC TROP MED & HYGIENE, Pages: 332-332, ISSN: 0002-9637

Conference paper

Moon RW, Taylor CJ, Bex C, Schepers R, Goulding D, Janse CJ, Waters AP, Baker DA, Billker Oet al., 2009, A Cyclic GMP Signalling Module That Regulates Gliding Motility in a Malaria Parasite, PLOS PATHOGENS, Vol: 5, ISSN: 1553-7366

Journal article

Doerig C, Baker D, Billker O, Blackman MJ, Chitnis C, Kumar DS, Heussler V, Holder AA, Kocken C, Krishna S, Langsley G, Lasonder E, Menard R, Meissner M, Pradel G, Ranford-Cartwright L, Sharma A, Sharma P, Tardieux T, Tatu U, Alano Pet al., 2009, SIGNALLING IN MALARIA PARASITES THE MALSIG CONSORTIUM, PARASITE, Vol: 16, Pages: 169-182, ISSN: 1252-607X

Journal article

Reininger L, Tewari R, Fennell C, Holland Z, Goldring D, Ranford-Cartwright L, Billker O, Doerig Cet al., 2009, An Essential Role for the Plasmodium Nek-2 Nima-related Protein Kinase in the Sexual Development of Malaria Parasites, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 284, Pages: 20858-20868

Journal article

Billker O, Lourido S, Sibley LD, 2009, Calcium-Dependent Signaling and Kinases in Apicomplexan Parasites, CELL HOST & MICROBE, Vol: 5, Pages: 612-622, ISSN: 1931-3128

Journal article

Doerig C, Billker O, Haystead T, Sharma P, Tobin AB, Waters NCet al., 2008, Protein kinases of malaria parasites: an update, TRENDS IN PARASITOLOGY, Vol: 24, Pages: 570-577, ISSN: 1471-4922

Journal article

McRobert L, Taylor CJ, Deng W, Fivelman QL, Cummings RM, Polley SD, Billker O, Baker DAet al., 2008, Gametogenesis in malaria parasites is mediated by the cGMP-dependent protein kinase, PLOS BIOLOGY, Vol: 6, Pages: 1243-1252, ISSN: 1545-7885

Journal article

Liu Y, Tewari R, Ning J, Blagborough AM, Garbom S, Pei J, Grishin NV, Steele RE, Sinden RE, Snell WJ, Billker Oet al., 2008, The conserved plant sterility gene HAP2 functions after attachment of fusogenic membranes in Chlamydomonas and Plasmodium gametes, GENES & DEVELOPMENT, Vol: 22, Pages: 1051-1068, ISSN: 0890-9369

Journal article

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