Publications
53 results found
Sinha A, Hughes KR, Modrzynska KK, et al., 2014, A cascade of DNA-binding proteins for sexual commitment and development in Plasmodium, NATURE, Vol: 507, Pages: 253-+, ISSN: 0028-0836
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- Citations: 229
Bauza K, Malinauskas T, Pfander C, et al., 2014, Efficacy of a Plasmodium vivax Malaria Vaccine Using ChAd63 and Modified Vaccinia Ankara Expressing Thrombospondin-Related Anonymous Protein as Assessed with Transgenic Plasmodium berghei Parasites, INFECTION AND IMMUNITY, Vol: 82, Pages: 1277-1286, ISSN: 0019-9567
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- Citations: 43
Brochet M, Collins MO, Smith TK, et al., 2014, Phosphoinositide Metabolism Links cGMP- Dependent Protein Kinase G to Essential Ca (2+) Signals at Key Decision Points in the Life Cycle of Malaria Parasites, PLOS BIOLOGY, Vol: 12, ISSN: 1544-9173
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- Citations: 128
Suarez C, Volkmann K, Gomes AR, et al., 2013, The Malarial Serine Protease SUB1 Plays an Essential Role in Parasite Liver Stage Development, PLOS PATHOGENS, Vol: 9, ISSN: 1553-7374
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- Citations: 22
Everitt AR, Clare S, McDonald JU, et al., 2013, Defining the Range of Pathogens Susceptible to Ifitm3 Restriction Using a Knockout Mouse Model, PLOS ONE, Vol: 8, ISSN: 1932-6203
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- Citations: 48
Frenal K, Tay CL, Mueller C, et al., 2013, Global Analysis of Apicomplexan Protein S-Acyl Transferases Reveals an Enzyme Essential for Invasion, TRAFFIC, Vol: 14, Pages: 895-911, ISSN: 1398-9219
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- Citations: 58
Ning J, Otto TD, Pfander C, et al., 2013, Comparative genomics in Chlamydomonas and Plasmodium identifies an ancient nuclear envelope protein family essential for sexual reproduction in protists, fungi, plants, and vertebrates, GENES & DEVELOPMENT, Vol: 27, Pages: 1198-1215, ISSN: 0890-9369
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- Citations: 52
Pfander C, Anar B, Brochet M, et al., 2013, Recombination-mediated genetic engineering of Plasmodium berghei DNA., Methods Mol Biol, Vol: 923, Pages: 127-138
DNA of Plasmodium berghei is difficult to manipulate in Escherichia coli by conventional restriction and ligation methods due to its high content of adenine and thymine (AT) nucleotides. This limits our ability to clone large genes and to generate complex vectors for modifying the parasite genome. We here describe a protocol for using lambda Red recombinase to modify inserts of a P. berghei genomic DNA library constructed in a linear, low-copy, phage-derived vector. The method uses primer extensions of 50 bp, which provide sufficient homology for an antibiotic resistance marker to recombine efficiently with a P. berghei genomic DNA insert in E. coli. In a subsequent in vitro Gateway reaction the bacterial marker is replaced with a cassette for selection in P. berghei. The insert is then released and used for transfection. The basic techniques we describe here can be adapted to generate highly efficient vectors for gene deletion, tagging, targeted mutagenesis, or genetic complementation with larger genomic regions.
Pino P, Sebastian S, Kim EA, et al., 2012, A Tetracycline-Repressible Transactivator System to Study Essential Genes in Malaria Parasites, CELL HOST & MICROBE, Vol: 12, Pages: 824-834, ISSN: 1931-3128
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- Citations: 69
Volkmann K, Pfander C, Burstroem C, et al., 2012, The Alveolin IMC1h Is Required for Normal Ookinete and Sporozoite Motility Behaviour and Host Colonisation in Plasmodium berghei, PLOS ONE, Vol: 7, ISSN: 1932-6203
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- Citations: 38
Sebastian S, Brochet M, Collins MO, et al., 2012, A Plasmodium Calcium-Dependent Protein Kinase Controls Zygote Development and Transmission by Translationally Activating Repressed mRNAs, CELL HOST & MICROBE, Vol: 12, Pages: 9-19, ISSN: 1931-3128
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- Citations: 113
Ojo KK, Pfander C, Mueller NR, et al., 2012, Transmission of malaria to mosquitoes blocked by bumped kinase inhibitors, JOURNAL OF CLINICAL INVESTIGATION, Vol: 122, Pages: 2301-2305, ISSN: 0021-9738
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- Citations: 76
Murphy RC, Ojo KK, Billker O, et al., 2012, BUMPED KINASE INHIBITORS BLOCK MALARIA TRANSMISSION TO MOSQUITOES, Western Regional Meeting, Publisher: LIPPINCOTT WILLIAMS & WILKINS, Pages: 195-195, ISSN: 1081-5589
Murphy RC, Ojo KK, Billker O, et al., 2012, BUMPED KINASE INHIBITORS BLOCK MALARIA TRANSMISSION TO MOSQUITOES, Western Regional Meeting, Publisher: LIPPINCOTT WILLIAMS & WILKINS, Pages: 169-170, ISSN: 1081-5589
Pfander C, Anar B, Schwach F, et al., 2011, A scalable pipeline for highly effective genetic modification of a malaria parasite, NATURE METHODS, Vol: 8, Pages: 1078-+, ISSN: 1548-7091
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- Citations: 59
Raabe A, Berry L, Sollelis L, et al., 2011, Genetic and transcriptional analysis of phosphoinositide-specific phospholipase C in Plasmodium, EXPERIMENTAL PARASITOLOGY, Vol: 129, Pages: 75-80, ISSN: 0014-4894
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- Citations: 20
Raabe AC, Wengelnik K, Billker O, et al., 2011, Multiple roles for Plasmodium berghei phosphoinositide-specific phospholipase C in regulating gametocyte activation and differentiation, CELLULAR MICROBIOLOGY, Vol: 13, Pages: 955-966, ISSN: 1462-5814
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- Citations: 39
Ramos TN, Darley MM, Hu X, et al., 2011, Cutting Edge: The Membrane Attack Complex of Complement Is Required for the Development of Murine Experimental Cerebral Malaria, JOURNAL OF IMMUNOLOGY, Vol: 186, Pages: 6657-6660, ISSN: 0022-1767
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- Citations: 19
Baum J, Billker O, Bousema T, et al., 2011, A Research Agenda for Malaria Eradication: Basic Science and Enabling Technologies, PLOS MEDICINE, Vol: 8, ISSN: 1549-1676
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- Citations: 34
Tewari R, Straschil U, Bateman A, et al., 2010, The Systematic Functional Analysis of Plasmodium Protein Kinases Identifies Essential Regulators of Mosquito Transmission, CELL HOST & MICROBE, Vol: 8, Pages: 377-387, ISSN: 1931-3128
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- Citations: 176
Doerig C, Billker O, 2010, A parasite calcium switch and Achilles' heel revealed, NATURE STRUCTURAL & MOLECULAR BIOLOGY, Vol: 17, Pages: 541-543, ISSN: 1545-9993
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- Citations: 2
Raabe AC, Billker O, Vial HJ, et al., 2009, Quantitative assessment of DNA replication to monitor microgametogenesis in Plasmodium berghei, MOLECULAR AND BIOCHEMICAL PARASITOLOGY, Vol: 168, Pages: 172-176, ISSN: 0166-6851
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- Citations: 24
Religa AA, Carret C, Feltwell T, et al., 2009, AN UNEXPECTED ROLE OF SIR2A IN THE LIFE CYCLE OF MALARIA PARASITES, 58th Annual Meeting of the American-Society-of-Tropical-Medicine-and-Hygiene, Publisher: AMER SOC TROP MED & HYGIENE, Pages: 332-332, ISSN: 0002-9637
Moon RW, Taylor CJ, Bex C, et al., 2009, A Cyclic GMP Signalling Module That Regulates Gliding Motility in a Malaria Parasite, PLOS PATHOGENS, Vol: 5, ISSN: 1553-7366
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- Citations: 128
Doerig C, Baker D, Billker O, et al., 2009, SIGNALLING IN MALARIA PARASITES THE MALSIG CONSORTIUM, PARASITE, Vol: 16, Pages: 169-182, ISSN: 1252-607X
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- Citations: 29
Reininger L, Tewari R, Fennell C, et al., 2009, An Essential Role for the Plasmodium Nek-2 Nima-related Protein Kinase in the Sexual Development of Malaria Parasites, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 284, Pages: 20858-20868
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- Citations: 67
Billker O, Lourido S, Sibley LD, 2009, Calcium-Dependent Signaling and Kinases in Apicomplexan Parasites, CELL HOST & MICROBE, Vol: 5, Pages: 612-622, ISSN: 1931-3128
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- Citations: 233
Doerig C, Billker O, Haystead T, et al., 2008, Protein kinases of malaria parasites: an update, TRENDS IN PARASITOLOGY, Vol: 24, Pages: 570-577, ISSN: 1471-4922
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- Citations: 91
McRobert L, Taylor CJ, Deng W, et al., 2008, Gametogenesis in malaria parasites is mediated by the cGMP-dependent protein kinase, PLOS BIOLOGY, Vol: 6, Pages: 1243-1252, ISSN: 1545-7885
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- Citations: 155
Liu Y, Tewari R, Ning J, et al., 2008, The conserved plant sterility gene HAP2 functions after attachment of fusogenic membranes in Chlamydomonas and Plasmodium gametes, GENES & DEVELOPMENT, Vol: 22, Pages: 1051-1068, ISSN: 0890-9369
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- Citations: 196
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