Imperial College London
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Dr Tolga Bozkurt

Faculty of Natural Sciences, Department of Life Sciences

Reader in Molecular Plant-Microbe
 
 
 
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Contact

 

+44 (0)20 7594 5381o.bozkurt

 
 
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Location

 

6167Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Duggan:2021:10.1073/pnas.2104997118,
author = {Duggan, C and Moratto, E and Savage, Z and Hamilton, E and Adachi, H and Wu, C-H and Leary, AY and Tumtas, Y and Rothery, SM and Maqbool, A and Nohut, S and Martin, TR and Kamoun, S and Bozkurt, O},
doi = {10.1073/pnas.2104997118},
journal = {Proceedings of the National Academy of Sciences of USA},
pages = {1--12},
title = {Dynamic localization of a helper NLR at the plant-pathogen interface underpins pathogen recognition},
url = {http://dx.doi.org/10.1073/pnas.2104997118},
volume = {118},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Plants employ sensor-helper pairs of NLR immune receptors to recognize pathogen effectors and activate immune responses (1). Yet the subcellular localization of NLRs pre- and post-activation during pathogen infection remains poorly understood. Here we show that NRC4, from the ‘NRC’ solanaceous helper NLR family (1), undergoes dynamic changes in subcellular localization by shuttling to and from the plant-pathogen haustorium interface established during infection by the Irish potato famine pathogen Phytophthora infestans. Specifically, prior to activation, NRC4 accumulates at the extra-haustorial membrane (EHM), presumably to mediate response to perihaustorial effectors, that are recognized by NRC4- dependent sensor NLRs. However not all NLRs accumulate at the EHM, as the closely related helper NRC2, and the distantly related ZAR1, did not accumulate at the EHM. NRC4 required an intact N-terminal coiled coil domain to accumulate at the EHM, whereas the functionally conserved MADA motif implicated in cell death activation and membrane insertion was dispensable for this process. Strikingly, a constitutively autoactive NRC4 mutant did not accumulate at the EHM and showed punctate distribution that mainly associated with the plasma membrane, suggesting that post-activation, NRC4 may undergo a conformation switch to form clusters that do not preferentially associate with the EHM. When NRC4 is activated by a sensor NLR during infection however, NRC4 forms puncta mainly at the EHM and to a lesser extent at the plasma membrane. We conclude that following activation at the EHM, NRC4 may spread to other cellular membranes from its primary site of activation to trigger immune responses.
AU  - Duggan,C
AU  - Moratto,E
AU  - Savage,Z
AU  - Hamilton,E
AU  - Adachi,H
AU  - Wu,C-H
AU  - Leary,AY
AU  - Tumtas,Y
AU  - Rothery,SM
AU  - Maqbool,A
AU  - Nohut,S
AU  - Martin,TR
AU  - Kamoun,S
AU  - Bozkurt,O
DO  - 10.1073/pnas.2104997118
EP  - 12
PY  - 2021///
SN  - 0027-8424
SP  - 1
TI  - Dynamic localization of a helper NLR at the plant-pathogen interface underpins pathogen recognition
T2  - Proceedings of the National Academy of Sciences of USA
UR  - http://dx.doi.org/10.1073/pnas.2104997118
UR  - https://www.pnas.org/content/118/34/e2104997118/
UR  - http://hdl.handle.net/10044/1/90280
VL  - 118
ER  -
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