Imperial College London
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Dr Tolga Bozkurt

Faculty of Natural Sciences, Department of Life Sciences

Reader in Molecular Plant-Microbe
 
 
 
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Contact

 

+44 (0)20 7594 5381o.bozkurt

 
 
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Location

 

6167Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Maqbool:2016:10.1074/jbc.M116.744995,
author = {Maqbool, A and Hughes, RK and Dagdas, YF and Tregidgo, N and Zess, E and Belhaj, K and Round, A and Bozkurt, TO and Kamoun, S and Banfield, MJ},
doi = {10.1074/jbc.M116.744995},
journal = {Journal of Biological Chemistry},
pages = {20270--20282},
title = {Structural Basis of Host Autophagy-related Protein 8 (ATG8) Binding by the Irish Potato Famine Pathogen Effector Protein PexRD54},
url = {http://dx.doi.org/10.1074/jbc.M116.744995},
volume = {291},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Filamentous plant pathogens deliver effector proteins to host cells to promote infection. The Phytophthora infestans RXLR-type effector PexRD54 binds potato ATG8 via its ATG8 family-interacting motif (AIM) and perturbs host-selective autophagy. However, the structural basis of this interaction remains unknown. Here, we define the crystal structure of PexRD54, which includes a modular architecture, including five tandem repeat domains, with the AIM sequence presented at the disordered C terminus. To determine the interface between PexRD54 and ATG8, we solved the crystal structure of potato ATG8CL in complex with a peptide comprising the effector's AIM sequence, and we established a model of the full-length PexRD54-ATG8CL complex using small angle x-ray scattering. Structure-informed deletion of the PexRD54 tandem domains reveals retention of ATG8CL binding in vitro and in planta This study offers new insights into structure/function relationships of oomycete RXLR effectors and how these proteins engage with host cell targets to promote disease.
AU  - Maqbool,A
AU  - Hughes,RK
AU  - Dagdas,YF
AU  - Tregidgo,N
AU  - Zess,E
AU  - Belhaj,K
AU  - Round,A
AU  - Bozkurt,TO
AU  - Kamoun,S
AU  - Banfield,MJ
DO  - 10.1074/jbc.M116.744995
EP  - 20282
PY  - 2016///
SN  - 1083-351X
SP  - 20270
TI  - Structural Basis of Host Autophagy-related Protein 8 (ATG8) Binding by the Irish Potato Famine Pathogen Effector Protein PexRD54
T2  - Journal of Biological Chemistry
UR  - http://dx.doi.org/10.1074/jbc.M116.744995
UR  - http://hdl.handle.net/10044/1/41465
VL  - 291
ER  -
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