Publications
172 results found
Shaw KP, Brooks NJ, Clarke JA, et al., 2012, Pressure – temperature phase behaviour of natural sphingomyelin extracts, Soft Matter, Vol: 8, Pages: 1070-1078
Sphingomyelin is the only sphingolipid occurring naturally in mammalian cells and can form up to 50% of the total phospholipid content of the myelin sheath which surrounds nerves. Having predominantly long, saturated acyl chains, it has a relatively high chain melting temperature and has been strongly associated with formation of lipid microdomains. Here, the lyotropic phase behaviour of sphingomyelin from three different natural sources (bovine brain, egg yolk and milk) in excess water is studied as a function of temperature and pressure by small- and wide-angle X-ray scattering, and solid state NMR. The different hydrocarbon chain length distributions of the three lipid extracts results in significant differences in their gel phase structure; both the bovine brain and egg yolk sphingomyelins can form a ripple gel phase but milk sphingomyelin forms an interdigitated gel phase due to the high degree of chain mismatch in its longer hydrocarbon chain components.
Lanigan PMP, Munro I, Grace EJ, et al., 2012, Dynamical hologram generation for high speed optical trapping of smart droplet microtools, Biomedical Optics Express, Vol: 3, Pages: 1609-1609
Salehi-Reyhani A, Kaplinsky J, Burgin E, et al., 2011, A first step towards practical single cell proteomics: a microfluidic antibodycapture chip with TIRF detection, Lab Chip, Vol: 11, Pages: 1256-1261
We have developed a generic platform to undertake the analysis of protein copy number from singlecells. The approach described here is ‘all-optical’ whereby single cells are manipulated into separate analysis chambers using an optical trap; single cells are lysed by a shock wave caused by laser-induced microcavitation, and the protein released from a single cell is measured by total internal reflection microscopy as it is bound to micro-printed antibody spots within the device. The platform was tested using GFP transfected cells and the relative precision of the measurement method was determined to be 88%. Single cell measurements were also made on a breast cancer cell line to measure the relative levels of unlabelled human tumour suppressor protein p53 using a chip incorporating an antibody sandwich assay format. These results suggest that this is a viable method for measuring relative protein levels in single cells.
Brooks NJ, Ces O, Templer RH, et al., 2011, Pressure effects on lipid membrane structure and dynamics, CHEMISTRY AND PHYSICS OF LIPIDS, Vol: 164, Pages: 89-98, ISSN: 0009-3084
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- Citations: 54
Child C, Long N, Ces O, et al., 2011, Investigating non-specific binding using a cell assay and LC/MS, JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS, Vol: 54, Pages: S124-S124, ISSN: 0362-4803
Macey R, Ces O, Gee A, et al., 2011, Predicting and measuring the <i>in vitro</i> non-specific binding of a series of drug analogues, JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS, Vol: 54, Pages: S126-S126, ISSN: 0362-4803
Tyler AII, Shearman GC, Brooks NJ, et al., 2011, Hydrostatic pressure effects on a hydrated lipid inverse micellar <i>Fd</i>3<i>m</i> cubic phase, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, Vol: 13, Pages: 3033-3038, ISSN: 1463-9076
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- Citations: 15
Shearman GC, Brooks NJ, Tiddy GJT, et al., 2011, A lyotropic inverse ribbon phase in a branched-chain polyoxyethylene surfactant: pressure effects, SOFT MATTER, Vol: 7, Pages: 4386-4390, ISSN: 1744-683X
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- Citations: 10
Corsi J, Hawtin RW, Ces O, et al., 2010, DNA Lipoplexes: Formation of the Inverse Hexagonal Phase Observed by Coarse-Grained Molecular Dynamics Simulation, LANGMUIR, Vol: 26, Pages: 12119-12125, ISSN: 0743-7463
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- Citations: 34
Brooks NJ, Gauthe BLLE, Terrill NJ, et al., 2010, Automated high pressure cell for pressure jump x-ray diffraction, REVIEW OF SCIENTIFIC INSTRUMENTS, Vol: 81, ISSN: 0034-6748
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- Citations: 61
Corsi J, Ces O, Khalid S, et al., 2010, Dna Lipoplexes: Prediction of Phase Architectures Using Cg Simulations and Experimental Validation, BIOPHYSICAL JOURNAL, Vol: 98, Pages: 76A-76A, ISSN: 0006-3495
Tyler AII, Shearman GC, Brooks NJ, et al., 2010, High Pressure Static and Time-Resolved X-Ray Studies of Inverse Phases in Cholesterol / Lipid Mixtures, BIOPHYSICAL JOURNAL, Vol: 98, Pages: 231A-231A, ISSN: 0006-3495
Goehring N, Margineanu A, Templer R, et al., 2010, Novel Probes for Sensing Lateral Stress in Membranes, BIOPHYSICAL JOURNAL, Vol: 98, Pages: 667A-667A, ISSN: 0006-3495
Kulkarni CV, Seddon AM, Ces O, et al., 2010, Evidence that membrane curvature distorts the tertiary structure of bacteriorhodopsin, SOFT MATTER, Vol: 6, Pages: 4339-4341, ISSN: 1744-683X
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- Citations: 14
Shearman GC, Tyler AII, Brooks NJ, et al., 2010, Ordered micellar and inverse micellar lyotropic phases, LIQUID CRYSTALS, Vol: 37, Pages: 679-694, ISSN: 0267-8292
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- Citations: 49
Stanley CE, Elvira KS, Niu XZ, et al., 2010, A microfluidic approach for high-throughput droplet interface bilayer (DIB) formation, CHEMICAL COMMUNICATIONS, Vol: 46, Pages: 1620-1622, ISSN: 1359-7345
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- Citations: 71
Shearman GC, Ces O, Templer RH, 2010, Towards an understanding of phase transitions between inverse bicontinuous cubic lyotropic liquid crystalline phases, SOFT MATTER, Vol: 6, Pages: 256-262, ISSN: 1744-683X
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- Citations: 25
Kulkarni CV, Tang T-Y, Seddon AM, et al., 2010, Engineering bicontinuous cubic structures at the nanoscale-the role of chain splay, SOFT MATTER, Vol: 6, Pages: 3191-3194, ISSN: 1744-683X
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- Citations: 83
Peiro-Salvador T, Ces O, Templer RH, et al., 2009, Buffers May Adversely Affect Model Lipid Membranes: A Cautionary Tale, BIOCHEMISTRY, Vol: 48, Pages: 11149-11151, ISSN: 0006-2960
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- Citations: 25
Ces O, Casey DR, Sebai SC, et al., 2009, Evidence that drug molecules eat their way through membranes and the consequences for phospholipidosis, 50th International Conference on Bioscience of Lipids, Publisher: ELSEVIER IRELAND LTD, Pages: S13-S14, ISSN: 0009-3084
Shearman GC, Ugazio S, Soubiran L, et al., 2009, The lyotropic phase behaviour of ester quaternary surfactants, JOURNAL OF COLLOID AND INTERFACE SCIENCE, Vol: 331, Pages: 463-469, ISSN: 0021-9797
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- Citations: 9
Gauthe BLLE, Heron AJ, Seddon JM, et al., 2009, A high pressure cell for simultaneous osmotic pressure and x-ray diffraction measurements, REVIEW OF SCIENTIFIC INSTRUMENTS, Vol: 80, ISSN: 0034-6748
Shearman GC, Ugazio S, Soubiran L, et al., 2009, Factors Controlling the Stability of a Kinetically Hindered Lamellar-Lamellar Transition, JOURNAL OF PHYSICAL CHEMISTRY B, Vol: 113, Pages: 1948-1953, ISSN: 1520-6106
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- Citations: 5
Shearman GC, Tyler AII, Brooks NJ, et al., 2009, A 3-D Hexagonal Inverse Micellar Lyotropic Phase, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol: 131, Pages: 1678-+, ISSN: 0002-7863
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- Citations: 54
Niu XZ, Zhang B, Marszalek RT, et al., 2009, Droplet-based compartmentalization of chemically separated components in two-dimensional separations, CHEMICAL COMMUNICATIONS, Pages: 6159-6161, ISSN: 1359-7345
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- Citations: 84
Lanigan PMP, Ninkovic T, Chan K, et al., 2009, A microfluidic platform for probing single cell plasma membranes using optically trapped Smart Droplet Microtools (SDMs), LAB ON A CHIP, Vol: 9, Pages: 1096-1101, ISSN: 1473-0197
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- Citations: 11
Mulet X, Rosivatz E, Ho KK, et al., 2009, Spatial localization of PtdInsP2 in phase-separated giant unilamellar vesicles with a fluorescent PLC-delta 1 PH domain, Methods Mol.Biol., Vol: 462, Pages: 135-144
This chapter describes a method for the preparation of giant unilamellar vesicles containing phosphatidylinositol 4,5-bisphosphate that are larger than 20 microm in size. The phospholipids composition of the vesicular membrane is such that fluid lamellar and liquid-ordered or gel phases are formed and separate within the confines of one vesicle. It outlines the preparation of a protein fluorescent label, pleckstrin homology domain from phospholipase C-delta 1, that binds specifically to phosphatidylinositol 4,5-bisphosphate. Using fluorescence microscopy, the presence and spatial position of this phosphorylated phosphatidylinositol lipid on the lipid membrane have been located with the pleckstrin homology domain. We show that phosphatidylinositol 4,5-bisphosphate and the phospholipase C-delta 1 pleckstrin homology domain are located to the fluid phase of the vesicle membrane. This approach can therefore show how membrane physical properties can affect enzyme binding to phosphatidylinositol 4,5-bisphosphate and thus further the understanding of important membrane processes such as endocytosis
Seddon AM, Casey D, Law RV, et al., 2009, Drug interactions with lipid membranes, Chem. Soc. Rev., Vol: 38, Pages: 2509-2519
Templer RH, Ces O, 2008, New frontiers in single-cell analysis, JOURNAL OF THE ROYAL SOCIETY INTERFACE, Vol: 5, Pages: S111-S112, ISSN: 1742-5689
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- Citations: 31
Lanigan PMP, Chan K, Ninkovic T, et al., 2008, Spatially selective sampling of single cells using optically trapped fusogenic emulsion droplets: a new single-cell proteomic tool, JOURNAL OF THE ROYAL SOCIETY INTERFACE, Vol: 5, Pages: S161-S168, ISSN: 1742-5689
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- Citations: 15
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