Publications
172 results found
Elani Y, Law R, Ces O, 2014, Microfluidic generation of networked droplet collections and lipid membrane constructs, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Pages: 677-679, ISSN: 1556-5904
We report on microfluidic strategies to generate several multi-compartment membrane-basedstructures, including droplet interface bilayer networks and multi-compartment vesicles. Thesedevelopments allow the current status quo— where microdroplets are used as isolated vessels— to bechanged. By linking droplets together with lipid membranes, higher order systems can be generated, withparticular ramifications for bottom-up synthetic biology and for functional droplet-based microreactorsand biodevices.
Carreras P, Elani Y, Law R, et al., 2014, A droplet trapping microfluidic device for the study of mass-transport across droplet interface bilayers, MicroTAS 2014
Carreras P, Law RV, Brooks NJ, et al., 2014, Microfluidic generation of droplet interface bilayer networks incorporating real-time size sorting in linear and non-linear configurations, Biomicrofluidics, Vol: 8, ISSN: 1932-1058
In this study, a novel droplet based microfluidic method for the generation of different sized droplet interface bilayers is reported. A microfluidic platform was designed, which allows the generation and packing of picoliter lipid coated water droplets. Droplets were generated by hydrodynamic focusing coupled with selective transport along grooves according to their size. A trapping structure at the end of the groove and a fine control of the flow pressures allowed for the droplets to be successfully trapped and aligned on demand. This technology facilitates the fine control of droplet size production as well as the generation of extended networks from a variety of lipids including 1,2-diphytanoyl-sn-glycero-3- phosphocholine and 1,2-dioleoyl-sn-glycero-3-phosphocholine in linear and non- linear configurations, which is vital to the application of Droplet Interface Bilayers to biological network construction on-chip.
Barriga HMG, Booth P, Haylock S, et al., 2014, Droplet interface bilayer reconstitution and activity measurement of the mechanosensitive channel of large conductance from Escherichia coli, Journal of the Royal Society Interface, Vol: 11, ISSN: 1742-5662
Droplet interface bilayers (DIBs) provide an exciting new platform for the study of membrane proteins in stable bilayers of controlled composition. To date, the successful reconstitution and activity measurement of membrane proteins in DIBs has relied on the use of the synthetic lipid 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC). We report the functional reconstitution of the mechanosensitive channel of large conductance (MscL) into DIBs composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), a lipid of significantly greater biological relevance than DPhPC. MscL functionality has been demonstrated using a fluorescence-based assay, showing that dye flow occurs across the DIB when MscL is gated by the cysteine reactive chemical 2-(trimethylammonium)ethyl methane thiosulfonate bromide (MTSET). MscL has already been the subject of a number of studies investigating its interaction with the membrane. We propose that this method will pave the way for future MscL studies looking in detail at the effects of controlled composition or membrane asymmetry on MscL activity using biologically relevant lipids and will also be applicable to other lipid–protein systems, paving the way for the study of membrane proteins in DIBs with biologically relevant lipids.
Burgin E, Salehi-Reyhani A, Barclay M, et al., 2014, Absolute quantification of protein copy number using a single-molecule-sensitive microarray, ANALYST, Vol: 139, Pages: 3235-3244, ISSN: 0003-2654
Casey D, Charalambous K, Gee A, et al., 2014, Amphiphilic drug interactions with model cellular membranes are influenced by lipid chain-melting temperature, JOURNAL OF THE ROYAL SOCIETY INTERFACE, Vol: 11, ISSN: 1742-5689
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- Citations: 15
Tang T-YD, Seddon AM, Jeworrek C, et al., 2014, The effects of pressure and temperature on the energetics and pivotal surface in a monoacylglycerol/water gyroid inverse bicontinuous cubic phase, SOFT MATTER, Vol: 10, Pages: 3009-3015, ISSN: 1744-683X
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- Citations: 10
Schrems A, Phillips J, Casey D, et al., 2014, The grab-and-drop protocol: a novel strategy for membrane protein isolation and reconstitution from single cells, ANALYST, Vol: 139, Pages: 3296-3304, ISSN: 0003-2654
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- Citations: 8
Salehi-Reyhani A, Sharma S, Burgin E, et al., 2014, Scaling advantages and constraints in miniaturized capture assays for single cell protein analysis (vol 13, pg 2066, 2013), LAB ON A CHIP, Vol: 14, Pages: 3430-3430, ISSN: 1473-0197
Miller D, Booth PJ, Seddon JM, et al., 2013, Protocell design through modular compartmentalization, JOURNAL OF THE ROYAL SOCIETY INTERFACE, Vol: 10, ISSN: 1742-5689
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- Citations: 16
Purushothaman S, Gauthe BLLE, Brooks NJ, et al., 2013, Automated laboratory based X-ray beamline with multi-capillary sample chamber, REVIEW OF SCIENTIFIC INSTRUMENTS, Vol: 84, ISSN: 0034-6748
Elani Y, Gee A, Law RV, et al., 2013, Manufacturing vesicles with internal bilayer partitions: a novel unit for synthetic biology, 9th European-Biophysical-Societies-Association Congress, Publisher: SPRINGER, Pages: S55-S55, ISSN: 0175-7571
Brooks N, Barriga H, McCarthy N, et al., 2013, Dynamic membrane structures at high pressure, 9th European-Biophysical-Societies-Association Congress, Publisher: SPRINGER, Pages: S118-S118, ISSN: 0175-7571
Tyler AII, Shearman GC, Parsons ES, et al., 2013, Tuning curvature in inverse micellar and bicontinuous cubic phases, 9th European-Biophysical-Societies-Association Congress, Publisher: SPRINGER, Pages: S140-S140, ISSN: 0175-7571
Kirsten ML, Baron RA, Seabra MC, et al., 2013, Rab1a and Rab5a preferentially bind to binary lipid compositions with higher stored curvature elastic energy, MOLECULAR MEMBRANE BIOLOGY, Vol: 30, Pages: 303-314, ISSN: 0968-7688
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- Citations: 10
Elani Y, Gee A, Law RV, et al., 2013, Engineering multi-compartment vesicle networks, CHEMICAL SCIENCE, Vol: 4, Pages: 3332-3338, ISSN: 2041-6520
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- Citations: 76
Hargreaves AL, Kirby AK, Bain CD, et al., 2013, An optical platform for the production, trapping, manipulation and visualization of ultra-low interfacial tension emulsion droplets, Conference on Optical Trapping and Optical Micromanipulation X, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X
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- Citations: 1
Kulkarni CV, Ces O, Templer RH, et al., 2013, Pressure effects on a protein-lipid model membrane, SOFT MATTER, Vol: 9, Pages: 6525-6531, ISSN: 1744-683X
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- Citations: 8
Boehm CR, Freemont PS, Ces O, 2013, Design of a prototype flow microreactor for synthetic biology <i>in vitro</i>, LAB ON A CHIP, Vol: 13, Pages: 3426-3432, ISSN: 1473-0197
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- Citations: 25
Salehi-Reyhani A, Sharma S, Burgin E, et al., 2013, Scaling Advantages and Constraints in Miniaturized Capture Assays for Single Cell Protein Analysis, Lab on A Chip, Vol: 13, Pages: 2066-2074, ISSN: 1473-0197
Measuring protein expression in single cells is the basis of single cell proteomics. The sensitivity and dynamic range of a single cell immunoassay should ideally be such that proteins that are expressed between 1 – 106 copies per cell can be detected and counted. We have investigated the effect of miniaturizing antibody microarrays by reducing capture spot sizes from 100 μm to 15 μm using dip pen nanolithography. We demonstrate that protocols developed for printing and passivating antibody capture spots using conventional pin based contact printing can be directly transferred to dip-pen lithography whilst retaining the capture activity per unit area. Using a simple kinetic model, we highlight how the limit of detection and dynamic range of a sandwich immunoassay, respectively, increase and decrease when spot size is reduced. However, we show that reducing spot size is more effective than increasing assay chamber volume when seeking to multiplex such a microfluidic immunoassay. Although, we make particular reference to single cell microfluidic immunoassays, the topics discussed here are applicable to capture assays in general.
Wu Y, Stefl M, Olzynska A, et al., 2013, Molecular rheometry: direct determination of viscosity in L<sub>o</sub> and L<sub>d</sub> lipid phases <i>via</i> fluorescence lifetime imaging, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, Vol: 15, Pages: 14986-14993, ISSN: 1463-9076
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- Citations: 137
Wylie D, Casey D, Phillips J, et al., 2012, Novel nanotechnologies for multiple spatially and temporally resolved live single cell membrane sampling and analysis, FREE RADICAL BIOLOGY AND MEDICINE, Vol: 53, Pages: S127-S128, ISSN: 0891-5849
Salehi-Reyhani A, Barclay M, Ces O, et al., 2012, Towards Practical Single Cell Proteomics: A Microfluidic Antibody Capture Chip with TIRF Detection, FREE RADICAL BIOLOGY AND MEDICINE, Vol: 53, Pages: S128-S128, ISSN: 0891-5849
Child C, Gee A, Long N, et al., 2012, Measuring non-specific binding of novel PET radioligands to determine structure-activity relationships between NSB and their physiochemical properties, 9th International Symposium on Functional Neuroreceptor Mapping of the Living Brain (NRM), Publisher: NATURE PUBLISHING GROUP, Pages: S108-S109, ISSN: 0271-678X
Tang TYD, Brooks NJ, Jeworrek C, et al., 2012, Hydrostatic Pressure Effects on the Lamellar to Gyroid Cubic Phase Transition of Monolinolein at Limited Hydration, Langmuir
Mak LH, Knott J, Scott KA, et al., 2012, Arylstibonic acids are potent and isoform-selective inhibitors of Cdc25a and Cdc25b phosphatases, BIOORGANIC & MEDICINAL CHEMISTRY, Vol: 20, Pages: 4371-4376, ISSN: 0968-0896
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- Citations: 11
Goyder MS, Willison KR, Klug DR, et al., 2012, Affinity chromatography and capillary electrophoresis for analysis of the yeast ribosomal proteins, BMB REPORTS, Vol: 45, Pages: 233-238, ISSN: 1976-6696
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- Citations: 3
Charalambous K, Booth PJ, Woscholski R, et al., 2012, Engineering <i>de Novo</i> Membrane-Mediated Protein-Protein Communication Networks, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol: 134, Pages: 5746-5749, ISSN: 0002-7863
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- Citations: 31
Elani Y, deMello AJ, Niu X, et al., 2012, Novel technologies for the formation of 2-D and 3-D droplet interface bilayer networks, Lab on a Chip, Vol: 12, Pages: 3514-3520, ISSN: 1473-0197
Droplet interface bilayer (DIB) networks have vast potential in the field of membrane biophysics,synthetic biology, and functional bio-electronics. However a technological bottleneck exists innetwork fabrication: existing methods are limited in terms of their automation, throughput,versatility, and ability to form well-defined 3-D networks. We have developed a series of novel andlow-cost methodologies which address these limitations. The first involves building DIB networksaround the contours of a microfluidic chip. The second uses flow rate and droplet size control toinfluence droplet packing geometries within a microfluidic chamber. The latter method enables thecontrolled formation of various 3-D network arrays consisting of thousands of interconnectedsymmetric and asymmetric lipid bilayers for the first time. Both approaches allow individual dropletposition and composition to be controlled, paving the way for complex on-chip functional networksynthesis.
Furse S, Brooks NJ, Seddon AM, et al., 2012, Lipid membrane curvature induced by distearoyl phosphatidylinositol 4-phosphate, Soft Matter
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