Imperial College London
Alternate

Professor Oscar Ces

Faculty of Natural Sciences, Department of Chemistry

Head of Department, August von Hofmann Chair of Chemistry
 
 
 
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Contact

 

+44 (0)20 7594 3754o.ces Website

 
 
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Location

 

G04AMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Zubaite:2022:10.1021/acsnano.2c02195,
author = {Zubaite, G and Hindley, JW and Ces, O and Elani, Y},
doi = {10.1021/acsnano.2c02195},
journal = {ACS Nano},
title = {Dynamic reconfiguration of subcompartment architectures in artificial cells.},
url = {http://dx.doi.org/10.1021/acsnano.2c02195},
volume = {16},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Artificial cells are minimal structures constructed from biomolecular building blocks designed to mimic cellular processes, behaviors, and architectures. One near-ubiquitous feature of cellular life is the spatial organization of internal content. We know from biology that organization of content (including in membrane-bound organelles) is linked to cellular functions and that this feature is dynamic: the presence, location, and degree of compartmentalization changes over time. Vesicle-based artificial cells, however, are not currently able to mimic this fundamental cellular property. Here, we describe an artificial cell design strategy that addresses this technological bottleneck. We create a series of artificial cell architectures which possess multicompartment assemblies localized either on the inner or on the outer surface of the artificial cell membrane. Exploiting liquid-liquid phase separation, we can also engineer spatially segregated regions of condensed subcompartments attached to the cell surface, aligning with coexisting membrane domains. These structures can sense changes in environmental conditions and respond by reversibly transitioning from condensed multicompartment layers on the membrane surface to a dispersed state in the cell lumen, mimicking the dynamic compartmentalization found in biological cells. Likewise, we engineer exosome-like subcompartments that can be released to the environment. We can achieve this by using two types of triggers: chemical (addition of salts) and mechanical (by pulling membrane tethers using optical traps). These approaches allow us to control the compartmentalization state of artificial cells on population and single-cell levels.
AU  - Zubaite,G
AU  - Hindley,JW
AU  - Ces,O
AU  - Elani,Y
DO  - 10.1021/acsnano.2c02195
PY  - 2022///
SN  - 1936-0851
TI  - Dynamic reconfiguration of subcompartment architectures in artificial cells.
T2  - ACS Nano
UR  - http://dx.doi.org/10.1021/acsnano.2c02195
UR  - https://www.ncbi.nlm.nih.gov/pubmed/35695383
UR  - https://pubs.acs.org/doi/10.1021/acsnano.2c02195
UR  - http://hdl.handle.net/10044/1/97452
VL  - 16
ER  -
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