Publications
221 results found
Yiangou Y, Facer P, Durrenberger P, et al., 2006, COX-2, CB2 and P2X7-immunoreactivities are increased in activated microglial cells/macrophages of multiple sclerosis and amyotrophic lateral sclerosis spinal cord, BMC NEUROLOGY, Vol: 6
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- Citations: 368
Durrenberger PF, Facer P, Casula MA, et al., 2006, Prostanoid receptor EP1 and Cox-2 in injured human nerves and a rat model of nerve injury: a time-course study, BMC Neurology, Vol: 6, ISSN: 1471-2377
BackgroundRecent studies show that inflammatory processes may contribute to neuropathic pain. Cyclooxygenase-2 (Cox-2) is an inducible enzyme responsible for production of prostanoids, which may sensitise sensory neurones via the EP1 receptor. We have recently reported that while macrophages infiltrate injured nerves within days of injury, they express increased Cox-2-immunoreactivity (Cox-2-IR) from 2 to 3 weeks after injury. We have now investigated the time course of EP1 and Cox-2 changes in injured human nerves and dorsal root ganglia (DRG), and the chronic constriction nerve injury (CCI) model in the rat.MethodsTissue sections were immunostained with specific antibodies to EP1, Cox-2, CD68 (human macrophage marker) or OX42 (rat microglial marker), and neurofilaments (NF), prior to image analysis, from the following: human brachial plexus nerves (21 to 196 days post-injury), painful neuromas (9 days to 12 years post-injury), avulsion injured DRG, control nerves and DRG, and rat CCI model tissues. EP1 and NF-immunoreactive nerve fibres were quantified by image analysis.ResultsEP1:NF ratio was significantly increased in human brachial plexus nerve fibres, both proximal and distal to injury, in comparison with uninjured nerves. Sensory neurones in injured human DRG showed a significant acute increase of EP1-IR intensity. While there was a rapid increase in EP1-fibres and CD-68 positive macrophages, Cox-2 increase was apparent later, but was persistent in human painful neuromas for years. A similar time-course of changes was found in the rat CCI model with the above markers, both in the injured nerves and ipsilateral dorsal spinal cord.ConclusionDifferent stages of infiltration and activation of macrophages may be observed in the peripheral and central nervous system following peripheral nerve injury. EP1 receptor level increase in sensory neurones, and macrophage infiltration, appears to precede increased Cox-2 expression by macrophages. However, other methods for
Htut M, Misra VP, Anand P, et al., 2006, Sensory recovery and pain phenomena following brachial plexus avulsion injury and surgical repairs, Clinical Neurosciences 2005 Meeting, Publisher: B M J PUBLISHING GROUP, Pages: 134-134, ISSN: 0022-3050
Facer P, Casula MA, Atherton D, et al., 2006, Advances in skin biopsy diagnosis of peripheral neuropathy with markers of novel sensory receptors, Clinical Neurosciences 2005 Meeting, Publisher: B M J PUBLISHING GROUP, Pages: 134-134, ISSN: 0022-3050
Durrenberger P, Facer P, Casula MA, et al., 2006, Nogo-A and Nogo-66 receptor in multiple sclerosis and amyotrophic lateral sclerosis spinal cord and injured human peripheral nerves, Clinical Neurosciences 2005 Meeting, Publisher: B M J PUBLISHING GROUP, Pages: 130-130, ISSN: 0022-3050
Atherton D, Facer P, Misra P, et al., 2006, Use of the novel contact heat evoked potential stimulator (CHEPS) for the diagnosis of small fibre neuropathy, Clinical Neurosciences 2005 Meeting, Publisher: B M J PUBLISHING GROUP, Pages: 134-134, ISSN: 0022-3050
Underwood J, Mukerji G, Yiangou Y, et al., 2006, Muscarinic receptors subtypes M2 and M3 in human urinary bladder disorders and their clinical correlations, 36th Annual Meeting of the International-Continence-Society, Publisher: WILEY-LISS, Pages: 609-610, ISSN: 0733-2467
Apostolidis A, Popat R, Yiangou Y, et al., 2005, Decreased sensory receptors P2X<sub>3</sub> and TRPV1 in suburothelial nerve fibers following intradetrusor injections of botulinum toxin for human detrusor overactivity, JOURNAL OF UROLOGY, Vol: 174, Pages: 977-982, ISSN: 0022-5347
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- Citations: 372
Renton T, Yiangou Y, Plumpton C, et al., 2005, Sodium channel Nav1.8 immunoreactivity in painful human dental pulp., BMC Oral Health, Vol: 5
BACKGROUND: The tetrodotoxin-resistant voltage-gated sodium channel Nav1.8 (SNS1/PN3) is expressed by nociceptors and may play a role in pain states. METHODS: Using specific antibodies for immunohistochemistry, we studied Nav1.8 immunoreactivity in human dental pulp in relation to the neuronal marker neurofilament. Human tooth pulp was extracted from teeth harvested from a total of twenty-two patients (fourteen without dental pain, eight patients with dental pain). RESULTS: Fibres immunoreactive for Nav1.8, were significantly increased on image analysis in the painful group: median (range) Nav1.8 to Neurofilament % area ratio, non-painful 0.059 (0.006-0.24), painful 0.265 (0.13-0.5), P = 0.0019. CONCLUSION: Nav1.8 sodium channels may thus represent a therapeutic target in trigeminal nerve pain states.
Yang LJS, Anand P, Birch R, 2005, Limb preference in children with obstetric brachial plexus palsy, PEDIATRIC NEUROLOGY, Vol: 33, Pages: 46-49, ISSN: 0887-8994
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- Citations: 29
Maker G, Ingo Y, Agawam S, et al., 2005, Increased capsaicin receptor TROVE in nerve fibres in painful bladder syndrome and its correlation with pain, BJU INTERNATIONAL, Vol: 95, Pages: 30-30, ISSN: 1464-4096
Chessell IP, Hatcher JP, Bountra C, et al., 2005, Disruption of the P2X<sub>7</sub> purinoceptor gene abolishes chronic inflammatory and neuropathic pain, PAIN, Vol: 114, Pages: 386-396, ISSN: 0304-3959
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- Citations: 628
Gopinath P, Wan E, Holdcroft A, et al., 2005, Increased capsaicin receptor TRPV1 in skin nerve fibres and related vanilloid receptors TRPV3 and TRPV4 in keratinocytes in human breast pain., BMC Women's Health, Vol: 5, Pages: 1-9, ISSN: 1472-6874
BACKGROUND: Breast pain and tenderness affects 70% of women at some time. These symptoms have been attributed to stretching of the nerves with increase in breast size, but tissue mechanisms are poorly understood. METHODS: Eighteen patients (n = 12 breast reduction and n = 6 breast reconstruction) were recruited and assessed for breast pain by clinical questionnaire. Breast skin biopsies from each patient were examined using immunohistological methods with specific antibodies to the capsaicin receptor TRPV1, related vanilloid thermoreceptors TRPV3 and TRPV4, and nerve growth factor (NGF). RESULTS: TRPV1-positive intra-epidermal nerve fibres were significantly increased in patients with breast pain and tenderness (TRPV1 fibres / mm epidermis, median [range] - no pain group, n = 8, 0.69 [0-1.27]; pain group, n = 10, 2.15 [0.77-4.38]; p = 0.0009). Nerve Growth Factor, which up-regulates TRPV1 and induces nerve sprouting, was present basal keratinocytes: some breast pain specimens also showed NGF staining in supra-basal keratinocytes. TRPV4-immunoreactive fibres were present in sub-epidermis but not significantly changed in painful breast tissue. Both TRPV3 and TRPV4 were significantly increased in keratinocytes in breast pain tissues; TRPV3, median [range] - no pain group, n = 6, 0.75 [0-2]; pain group, n = 11, 2 123, p = 0.008; TRPV4, median [range] - no pain group, n = 6, [0-1]; pain group, n = 11, 1 [0.5-2], p = 0.014). CONCLUSION: Increased TRPV1 intra-epidermal nerve fibres could represent collateral sprouts, or re-innervation following nerve stretch and damage by polymodal nociceptors. Selective TRPV1-blockers may provide new therapy in breast pain. The role of TRPV3 and TRPV4 changes in keratinocytes deserve further study.
Inglis JJ, Anand P, Facer P, et al., 2005, Exploring mechanisms of inflammatory hyperalgesia in murine collagen-induced arthritis, 25th European Workshop for Rheumatology Research, Publisher: BIOMED CENTRAL LTD, Pages: S44-S45, ISSN: 1478-6354
Apostolidis A, Brady CM, Yiangou Y, et al., 2005, Capsaicin receptor TRPV1 in urothelium of neurogenic human bladders and effect of intravesical resiniferatoxin, UROLOGY, Vol: 65, Pages: 400-405, ISSN: 0090-4295
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- Citations: 134
Matthews PJ, Aziz O, Facer P, et al., 2004, Increased capsaicin receptor TRPV1 nerve fibres in the inflamed human oesophagus, EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY, Vol: 16, Pages: 897-902, ISSN: 0954-691X
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- Citations: 199
Brady CM, Apostolidis A, Yiangou Y, et al., 2004, P2X3-immunoreactive nerve fibres in neurogenic detrusor overactivity and the effect of intravesical resiniferatoxin, EUROPEAN UROLOGY, Vol: 46, Pages: 247-253, ISSN: 0302-2838
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- Citations: 127
Casula MA, Facer P, Powell AJ, et al., 2004, Expression of the sodium channel β3 subunit in injured human sensory neurons, NEUROREPORT, Vol: 15, Pages: 1629-1632, ISSN: 0959-4965
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- Citations: 19
Ellaway PH, Anand P, Bergstrom EMK, et al., 2004, Towards improved clinical and physiological assessments of recovery in spinal cord injury: a clinical initiative, SPINAL CORD, Vol: 42, Pages: 325-337, ISSN: 1362-4393
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- Citations: 49
Bergerot A, Shortland PJ, Anand P, et al., 2004, Co-treatment with riluzole and GDNF is necessary for functional recovery after ventral root avulsion injury, EXPERIMENTAL NEUROLOGY, Vol: 187, Pages: 359-366, ISSN: 0014-4886
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- Citations: 73
Carlstedt T, Anand P, Htut M, et al., 2004, Restoration of hand function and so called "breathing arm" after intraspinal repair of C5-T1 brachial plexus avulsion injury. Case report., Neurosurg Focus, Vol: 16
This 9-year-old boy sustained a complete right-sided C5-T1 brachial plexus avulsion injury in a motorcycle accident. He underwent surgery 4 weeks after the accident. The motor-related nerve roots in all parts of the avulsed brachial plexus were reconnected to the spinal cord by reimplantation of peripheral nerve grafts. Recovery in the proximal part of the arm started 8 to 10 months later. Motor function was restored throughout the arm and also in the intrinsic muscles of the hand by 2 years postoperatively. The initial severe excruciating pain, typical after nerve root avulsions, disappeared completely with motor recovery. The authors observed good recruitment of regenerated motor units in all parts of the arm, but there were cocontractions. Transcranial magnetic stimulation produced response in all muscles, with prolonged latency and smaller amplitude compared with the intact side. There was inspiration-evoked muscle activity in proximal arm muscles--that is, the so-called "breathing arm" phenomenon. The issues of nerve regeneration after intraspinal reimplantation in a young individual, as well as plasticity and associated pain, are discussed. To the best of the authors' knowledge, the present case demonstrates, for the first time, that spinal cord surgery can restore hand function after a complete brachial plexus avulsion injury.
Rabert D, Xiao YY, Yiangou Y, et al., 2004, Plasticity of gene expression in injured human dorsal root ganglia revealed by GeneChip oligonucleotide microarrays, JOURNAL OF CLINICAL NEUROSCIENCE, Vol: 11, Pages: 289-299, ISSN: 0967-5868
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- Citations: 15
Tympanidis P, Casula MA, Yiangou Y, et al., 2004, Increased vanilloid receptor VR1 innervation in vulvodynia, EUROPEAN JOURNAL OF PAIN, Vol: 8, Pages: 129-133, ISSN: 1090-3801
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- Citations: 124
Brady CM, Apostolidis AN, Harper M, et al., 2004, Parallel changes in bladder suburothelial vanilloid receptor TRPV1 and pan-neuronal marker PGP9.5 immunoreactivity in patients with neurogenic detrusor overactivity after intravesical resiniferatoxin treatment, BJU INTERNATIONAL, Vol: 93, Pages: 770-776, ISSN: 1464-4096
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- Citations: 151
Cruccu G, Anand P, Attal N, et al., 2004, EFNS guidelines on neuropathic pain assessment, EUROPEAN JOURNAL OF NEUROLOGY, Vol: 11, Pages: 153-162, ISSN: 1351-5101
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- Citations: 349
Durrenberger PF, Facer P, Gray RA, et al., 2004, Cyclooxygenase-2 (Cox-2) in injured human nerve and a rat model of nerve injury, JOURNAL OF THE PERIPHERAL NERVOUS SYSTEM, Vol: 9, Pages: 15-25, ISSN: 1085-9489
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- Citations: 45
Apostolidis AN, Yiangou Y, Brady CM, et al., 2004, Endothelial nitric oxide synthase expression in neurogenic urinary bladders treated with intravesical resiniferatoxin, BJU INTERNATIONAL, Vol: 93, Pages: 336-340, ISSN: 1464-4096
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- Citations: 8
Quraishi NA, Taherzadeh O, McGregor AH, et al., 2004, Correlation of nerve root pain with dermatomal sensory threshold and back pain with spinal movement in single level lumbar spondylosis, JOURNAL OF BONE AND JOINT SURGERY-BRITISH VOLUME, Vol: 86B, Pages: 74-80, ISSN: 0301-620X
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- Citations: 15
Apostolidis A, Popat R, Yiangou Y, et al., 2004, A possible explanation for the exceptional efficacy of botulinum toxin treatment for detrusor overactivity, Joint Meeting of the International-Continence-Society/International-UroGynecological-Association, Publisher: WILEY-LISS, Pages: 608-609, ISSN: 0733-2467
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- Citations: 7
Anand P, 2004, Neurotrophic factors and their receptors in human sensory neuropathies, NGF AND RELATED MOLECULES IN HEALTH AND DISEASE, Vol: 146, Pages: 477-492, ISSN: 0079-6123
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- Citations: 111
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