Imperial College London
Alternate

ProfessorPraveenAnand

Faculty of Medicine, Department of Brain Sciences

Professor
 
 
 
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Contact

 

+44 (0)20 3313 3319p.anand

 
 
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Location

 

Area A Grd FloorUnknownHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Jochmann:2015:10.1186/s13075-015-0818-8,
author = {Jochmann, E and Boettger, MK and Anand, P and Schaible, HG},
doi = {10.1186/s13075-015-0818-8},
journal = {Arthritis Research and Therapy},
title = {Antigen-induced arthritis in rats is associated with increased growth-associated protein 43-positive intraepidermal nerve fibres remote from the joint.},
url = {http://dx.doi.org/10.1186/s13075-015-0818-8},
volume = {17},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - INTRODUCTION: Pain in arthritis may be experienced in regions outside the affected joint, and hyperalgesia may even be widespread. The spread of pain is usually attributed to mechanisms in the central nervous system. We investigated whether rats with antigen-induced arthritis (AIA) exhibit peripheral changes in skin innervation remote from the inflamed joint. METHODS: After immunization, unilateral AIA in the knee joint was induced in rats. Intraepidermal nerve fibre density was determined by immunohistochemical staining for protein gene product 9.5 (PGP 9.5) and for nerve fibres expressing calcitonin gene-related peptide (CGRP), substance P (SP), transient receptor potential vanilloid 1 (TRPV1; the heat and capsaicin receptor), β-tubulin, and growth-associated protein 43 (GAP-43; a marker of regenerating nerve fibres) in paw pad skin and back skin. Cluster of differentiation 11b (CD11b)-positive macrophages and CD3-positive T cells were quantified in skin, and macrophages were quantified in the lumbar dorsal root ganglia. In addition, pain-related behaviour was assessed. RESULTS: Intraepidermal nerve fibre density (PGP 9.5) and the numbers of fibres expressing CGRP, SP, TRPV1, or β-tubulin did not show a significant change in the acute (3 days) or chronic phase (21 days) of AIA compared with control rats that were only immunized. However, paw skin and back skin revealed a significantly higher number of nerve fibres expressing GAP-43 at both the acute and chronic stages of AIA. The skin of arthritic rats in these regions did not contain a greater density of CD11b and CD3 immune cells than the skin of control rats. Enhanced expression of GAP-43 in nerve fibres of the skin was not related to hyperalgesia in the joint, but it accompanied persistent secondary cutaneous hyperalgesia in the skin remote from the inflamed joint. CONCLUSIONS: Although the innervation of the skin remote from the joint did not show significant abnormalities of the other ner
AU  - Jochmann,E
AU  - Boettger,MK
AU  - Anand,P
AU  - Schaible,HG
DO  - 10.1186/s13075-015-0818-8
PY  - 2015///
SN  - 1478-6362
TI  - Antigen-induced arthritis in rats is associated with increased growth-associated protein 43-positive intraepidermal nerve fibres remote from the joint.
T2  - Arthritis Research and Therapy
UR  - http://dx.doi.org/10.1186/s13075-015-0818-8
UR  - http://hdl.handle.net/10044/1/27434
VL  - 17
ER  -
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