Publications
228 results found
Felkin LE, Taegtmeyer AB, Barton PJR, 2006, Real-time quantitative polymerase chain reaction in cardiac transplant research., Methods Mol Biol, Vol: 333, Pages: 305-330, ISSN: 1064-3745
The real-time quantitative polymerase chain reaction (PCR), an increasingly popular technique for the detection of DNA, combines a high degree of accuracy with extreme sensitivity. In this chapter we describe the use of real-time quantitative PCR in transplantation research in two areas in which this method is commonly applied: the accurate quantification of mRNA in tissue samples and genotyping of DNA. These are described in the context of cardiac transplantation, but they are of equal relevance to other areas of transplant biology.
Barton PJR, Felkin LE, Birks EJ, et al., 2005, Myocardial insulin-like growth factor-I gene expression during recovery from heart failure after combined left ventricular assist device and clenbuterol therapy, CIRCULATION, Vol: 112, Pages: I46-I50, ISSN: 0009-7322
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- Citations: 53
Birks EJ, Hall JL, Barton PJR, et al., 2005, Gene proriling changes in cytoskeletal proteins during clinical recovery after left ventricular-assist device support, CIRCULATION, Vol: 112, Pages: I57-I64, ISSN: 0009-7322
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- Citations: 82
SantalucĂa T, Sánchez-Feutrie M, Felkin LE, et al., 2005, Phenylephrine requires the TATA box to activate transcription of GLUT1 in neonatal rat cardiac myocytes, JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, Vol: 38, Pages: 677-684, ISSN: 0022-2828
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- Citations: 1
De Souza AI, Felkin LE, McCormack AM, et al., 2005, Sequential expression of three known protective genes in cardiac biopsies after transplantation, TRANSPLANTATION, Vol: 79, Pages: 584-590, ISSN: 0041-1337
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- Citations: 13
Birks EJ, Felkin LE, Hardy J, et al., 2005, Changes in myocardial pro- and antiinflammatory cytokines during and one year after successful LVAD explantation, JOURNAL OF HEART AND LUNG TRANSPLANTATION, Vol: 24, Pages: S101-S101, ISSN: 1053-2498
Taylor-Harris PM, Felkin LE, Birks EJ, et al., 2005, Expression of human membrane skeleton protein genes for protein 4.1 and βIIΣ2-spectrin assayed by real-time RT-PCR, CELLULAR & MOLECULAR BIOLOGY LETTERS, Vol: 10, Pages: 135-149, ISSN: 1425-8153
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- Citations: 16
Taegtmeyer AB, Crook AM, Barton PJR, et al., 2004, Reduced incidence of hypertension after heterotopic cardiac transplantation compared with orthotopic cardiac transplantation - Evidence that excision of the native heart contributes to post-transplant hypertension, JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, Vol: 44, Pages: 1254-1260, ISSN: 0735-1097
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- Citations: 14
Suzuki K, Murtuza B, Beauchamp JR, et al., 2004, Role of interleukin-1β in acute inflammation and graft death after cell transplantation to the heart, CIRCULATION, Vol: 110, Pages: II219-II224, ISSN: 0009-7322
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- Citations: 97
Taegtmeyer AB, Crook AM, Barton PJR, et al., 2004, Reduced incidence of hypertension after heterotopic cardiac transplantation compared with orthotopic cardiac transplantation, Journal of the American College of Cardiology, Vol: 44, Pages: 1254-1260, ISSN: 0735-1097
Cullen ME, Dellow KA, Barton PJR, 2004, Structure and regulation of human troponin genes, MOLECULAR AND CELLULAR BIOCHEMISTRY, Vol: 263, Pages: 81-90, ISSN: 0300-8177
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- Citations: 21
Cullen ME, Dellow KA, Barton PJR, 2004, Structure and regulation of human troponin genes., Mol Cell Biochem, Vol: 263, Pages: 81-90, ISSN: 0300-8177
The recent completion of a first draft of the human genome has allowed "in silico" genome browsing to become routine. Such computer-based research is now a useful adjunct to experiments based at the bench, and is accelerating gene discovery and the analysis and understanding of genes in their genomic contexts. This review summarises recent findings on genes encoding proteins of the troponin complex. We describe the organization of the three pairs of genes which encode isoforms of troponins I and T, and discuss how this relates to their evolution and regulation. Detailed analysis of the chromosomal context of the cardiac troponin I and slow skeletal troponin T genes reveals a region of densely packed differentially expressed genes, including new genes identified by automatic genome annotation. This information is discussed within the context of detailed analysis of the best-studied gene in this region, cardiac troponin I. In this way, we illustrate the uses to which a combination of conventional bench experiments and "in silico" analyses may be put in understanding the relationship between structure and function within the genome. (Mol Cell Biochem 263: 81-90, 2004).
Barton PJR, Felkin LE, Koban MU, et al., 2004, The slow skeletal muscle troponin T gene is expressed in developing and diseased human heart., Mol Cell Biochem, Vol: 263, Pages: 91-97, ISSN: 0300-8177
Cardiac muscle development is characterised by the activation of contractile protein genes and subsequent modulation of expression resulting, ultimately, in the formation of a mature four-chambered organ. Myocardial gene expression is also altered in the adult in response to pathological stimuli and this is thought to contribute to the altered contractile characteristics of the diseased heart. We have examined the expression of the slow skeletal troponin T (TnT) gene in the human heart during development and in disease using whole mount in situ hybridisation and real-time quantitative (TaqMan) polymerase chain reaction (PCR). Slow skeletal TnT mRNA shows transitory and regional expression in the early foetal heart, which occurs at different times in atria and ventricles. In ventricular myocardium, expression is seen in the outer epicardial layer at a time when the coronary circulation is being established. Expression was detected at low levels in the adult human heart and was significantly increased in end-stage heart failure. Similarly, expression was readily detectable during early rat heart development and was up-regulated in pressure overload hypertrophy in adult. Together these data show for the first time that slow skeletal TnT mRNA is readily detectable during early human heart development. They further suggest that slow skeletal TnT may be responsive to myocardial stress and that elevated levels may contribute to myocardial dysfunction in adult disease. (Mol Cell Biochem 263: 91-97, 2004).
Yacoub MH, Yuen AHY, Kalsi KAK, et al., 2004, C34T <i>AMP deaminase 1</i> gene mutation protects cardiac function in donors, TRANSPLANTATION, Vol: 77, Pages: 1621-1623, ISSN: 0041-1337
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- Citations: 9
Birks EJ, Felkin LE, Banner NR, et al., 2004, Increased toll-like receptor 4 in the myocardium of patients requiring left ventricular assist devices, JOURNAL OF HEART AND LUNG TRANSPLANTATION, Vol: 23, Pages: 228-235, ISSN: 1053-2498
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- Citations: 73
Taegtmeyer AB, Breen JB, Smith JD, et al., 2004, Increased incidence of acute rejection among cardiac transplant recipients possessing the Gln12STOP variant of adenosine monophosphate deaminase-1., American Transplant Congress, Publisher: BLACKWELL MUNKSGAARD, Pages: 311-311, ISSN: 1600-6135
Taegtmeyer AB, Breen JB, Pantelidis P, et al., 2004, The G2677T but not the C3435T polymorphism of the multidrug resistance gene 1 (MDR-1) affects cyclosporin dose requirements in stable adult heart transplant patients., American Transplant Congress, Publisher: BLACKWELL MUNKSGAARD, Pages: 526-526, ISSN: 1600-6135
Barton PJR, Felkin LE, Koban MU, et al., 2004, The slow skeletal muscle troponin T gene is expressed in developing and diseased human heart., Mol Cell Biochem, Vol: 263, Pages: 91-97
Barton PJR, Birks EJ, Felkin LE, et al., 2003, Increased expression of extracellular matrix regulators TIMP1 and MMP1 in deteriorating heart failure, JOURNAL OF HEART AND LUNG TRANSPLANTATION, Vol: 22, Pages: 738-744, ISSN: 1053-2498
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- Citations: 57
Terracciano CMN, Harding SE, Adamson D, et al., 2003, Changes in sarcolemmal Ca entry and sarcoplasmic reticulum Ca content in ventricular myocytes from patients with end-stage heart failure following myocardial recovery after combined pharmacological and ventricular assist device therapy, EUROPEAN HEART JOURNAL, Vol: 24, Pages: 1329-1339, ISSN: 0195-668X
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- Citations: 56
Felkin LE, Birks EJ, Yacoub MH, et al., 2003, Elevated myocardial TIMP1 expression correlates with interleukin-6 in patients with deteriorating heart failure, and is stimulated by interleukin-6 in vitro, Congress of the European-Society-of-Cardiology, Publisher: W B SAUNDERS CO LTD, Pages: 144-144, ISSN: 0195-668X
Weekes J, Morrison K, Mullen A, et al., 2003, Hyperubiquitination of proteins in dilated cardiomyopathy, PROTEOMICS, Vol: 3, Pages: 208-216, ISSN: 1615-9853
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- Citations: 168
Clerk A, Kemp TJ, Harrison JG, et al., 2002, Up-regulation of c-<i>jun</i> mRNA in cardiac myocytes requires the extracellular signal-regulated kinase cascade, but c-Jun N-terminal kinases are required for efficient up-regulation of c-Jun protein, BIOCHEMICAL JOURNAL, Vol: 368, Pages: 101-110, ISSN: 0264-6021
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- Citations: 53
Bhavsar PK, Felkin LE, Barton PJR, et al., 2002, Molecular and morphological effects of clenbuterol on neonatal rat cardiac myocytes in culture, Congress of the European-Society-of-Cardiology, Publisher: W B SAUNDERS CO LTD, Pages: 369-369, ISSN: 0195-668X
Taegtmeyer AB, Barton PJR, 2002, SNP at your own risk, EUROPEAN HEART JOURNAL, Vol: 23, Pages: 692-694, ISSN: 0195-668X
Brand NJ, Barton PJR, 2002, Myocardial molecular biology: an introduction, HEART, Vol: 87, Pages: 284-293, ISSN: 1355-6037
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- Citations: 3
Barton PJR, Moorman AFM, 2002, Molecular biology and cardiac development, Paediatric Cardiology, Editors: Anderson, Baker, Shinebourne, Rigby, Tynan, London, Publisher: Churchill Livingstone, Pages: 215-233, ISBN: 9780443079900
Barton PJR, Dellow KA, Bhavsar PK, et al., 2002, Regulation and organization of human troponin genes, Myofibrillogenesis, Boston, Publisher: Birkhauser, Pages: 129-141, ISBN: 9780817642266
Birks EJ, Latif N, Owen V, et al., 2001, Quantitative myocardial cytokine expression and activation of the apoptotic pathway in patients who require left ventricular assist devices, CIRCULATION, Vol: 104, Pages: I233-I240, ISSN: 0009-7322
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- Citations: 60
Birks EJ, Latif N, Owen V, et al., 2001, Quantitative myocardial cytokine expression and activation of the apoptotic pathway in patients who require left ventricular assist devices, Circulation, Vol: 104, ISSN: 0009-7322
Background - Molecular mechanisms underlying the deterioration of patients undergoing LV assist device (LVAD) implantation remain poorly understood. We studied the cytokines tumor necrosis factor (TNF)-α and interleukin (IL)-1β and IL-6 and the terminal stage of the apoptotic pathway in patients with decompensating heart failure who required LVAD support and compared them with patients with less severe heart failure undergoing elective heart transplantation. Methods and Results - Myocardial and serum samples from 23 patients undergoing LVAD implantation were compared with those from 36 patients undergoing elective heart transplantation. Myocardial TNF-α mRNA (1.71-fold; P<0.05) and protein (3.43±0.19 versus 2.95±0.10 pg/mg protein; P<0.05) were elevated in the LVAD patients. Immunocytochemistry demonstrated TNF expression in the myocytes. Serum TNF-α was also elevated (12.5±1.9 versus 4.0±0.4 pg/mL; P<0.0001) in the LVAD patients. IL-6 mRNA (2.57-fold higher; P<0.005) and protein (27.83±9.35 versus 4.26±1.24 pg/mg protein; P<0.001) were higher in the LVAD candidates, as was serum IL-6 (79.3±23.6 versus 7.1±1.6 pg/mL; P<0.0001). Interleukin-1β mRNA expression was 9.78-fold higher in the LVAD patients (P<0.001). iNOS mRNA expression was similar to that in advanced heart failure patients and was not further elevated in the LVAD patients. Levels of procaspase-9 (8.02±0.91 versus 6.16±0.43 oligodeoxynucleotide [OD] units; P<0.01), cleaved caspase-9 (10.02±1.0 versus 7.34±0.40 OD units; P<0.05), intact and spliced DFF-45 (4.58±0.75 versus 2.84±0.23 OD units; P±0.05) were raised in LVAD patients, but caspase-3 and human nuclease CPAN were not. Conclusions - Elevated TNF-α, IL-1β, and IL-6 and alterations in the apoptotic pathway were found in the myocardium and elevated TNF-α and IL-6 in serum of de
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