Publications
228 results found
Thomas PS, Kasahara H, Edmonson AM, et al., 2001, Elevated expression of Nkx-2.5 in developing myocardial conduction cells, ANATOMICAL RECORD, Vol: 263, Pages: 307-313, ISSN: 0003-276X
- Author Web Link
- Cite
- Citations: 52
Owen VJ, Burton PBJ, Mullen AJ, et al., 2001, Expression of RGS3, RGS4 and Gi alpha 2 in acutely failing donor hearts and end-stage heart failure, EUROPEAN HEART JOURNAL, Vol: 22, Pages: 1015-1020, ISSN: 0195-668X
- Author Web Link
- Cite
- Citations: 56
Dellow KA, Bhavsar PK, Brand NJ, et al., 2001, Identification of novel, cardiac-restricted transcription factors binding to a CACC-box within the human cardiac troponin I promoter, CARDIOVASCULAR RESEARCH, Vol: 50, Pages: 24-33, ISSN: 0008-6363
- Author Web Link
- Cite
- Citations: 19
Birks EJ, Burton PB, Owen V, et al., 2000, Elevated tumor necrosis factor-alpha and interleukin-6 in myocardium and serum of malfunctioning donor hearts., Circulation, Vol: 102, Pages: III352-III358
BACKGROUND: Myocardial dysfunction is a common and important problem in donor hearts. The mechanisms responsible remain unclear. We have studied the cytokines tumor necrosis factor (TNF)-alpha and interleukin-6 (IL-6) in the myocardium and serum from donors with myocardial dysfunction (unused donors) and compared them with donors with good ventricular function (used donors) and patients with advanced heart failure (HF). METHODS AND RESULTS: Clinical details and ventricular function were assessed in 46 donors (31 used, 15 unused). Real-time reverse transcription-polymerase chain reaction, Western blotting, and immunocytochemistry were performed on myocardium and immunoassays on serum. TNF-alpha mRNA was 1.6-fold higher in unused than in used donors (P:<0.005) and 1.74-fold higher than in 36 patients with HF. IL-6 mRNA was 2.4-fold higher in unused than in used donors (P:<0.0001) and 4.67-fold higher than in HF (P:<0.0001). Western blotting showed higher TNF-alpha in unused (218. 3+/-6.4, n=4 versus 187.3+/-5.4, n=3 OD units) than used donors (P:<0.05). TNF-alpha expression was localized to cardiac myocytes. Serum TNF-alpha was higher in unused (8.72+/-1.3 pg/mL, n=13) than in used (6.12+/-0.8 pg/mL, n=25, P:<0.05) donors and HF (4.0+/-0.4 pg/mL, n=17, P:<0.005). Serum TNF-alpha receptors did not differ between unused (4.3+/-0.8 and 8.6+/-1.6 ng/mL, n=10) and used (3. 5+/-0.4 and 6.5+/-1.1 ng/mL, n=24) donors. There was a trend for higher serum IL-6 in unused (16.5+/-2.9 pg/mL, n=9) compared with used (13.9+/-1.6 pg/mL, n=26, P:=NS) donors. CONCLUSIONS: This study documented an increase in the expression of TNF-alpha and IL-6 in the myocardium of all donor hearts that was more marked in the dysfunctional (unused) donor hearts. This was accompanied by similar changes in the serum. This might have important therapeutic implications.
Birks EJ, Burton PBJ, Owen V, et al., 2000, Elevated tumor necrosis factor-α and interleukin-6 in myocardium and serum of malfunctioning donor hearts, CIRCULATION, Vol: 102, Pages: 352-358, ISSN: 0009-7322
- Author Web Link
- Cite
- Citations: 84
Birks EJ, Latif N, Bowles C, et al., 2000, Measurement of cytokine levels and activation of the apoptotic pathway in patients requiring left ventricular assist devices (LVAD): Implications for timing of implantation, CIRCULATION, Vol: 102, Pages: 818-818, ISSN: 0009-7322
- Author Web Link
- Cite
- Citations: 1
Birks EJ, Owen VJ, Latif N, et al., 2000, A possible role for tumour necrosis factor (TNF)-α, Gi alpha and activation of the apoptotic pathway in dysfunctional donor hearts, CIRCULATION, Vol: 102, Pages: 738-738, ISSN: 0009-7322
Barton PJR, Mullen AJ, Cullen ME, et al., 2000, Genes encoding troponin I and troponin T are organized as three paralogous pairs in the mouse genome, MAMMALIAN GENOME, Vol: 11, Pages: 926-929, ISSN: 0938-8990
- Author Web Link
- Cite
- Citations: 10
Burton PBJ, Owen VJ, Hafizi S, et al., 2000, Vascular endothelial growth factor release following coronary artery bypass surgery: extracorporeal circulation versus 'beating heart' surgery, EUROPEAN HEART JOURNAL, Vol: 21, Pages: 1708-1713, ISSN: 0195-668X
- Author Web Link
- Cite
- Citations: 18
Mullen AJ, Barton PJR, 2000, Structural characterization of the human fast skeletal muscle troponin I gene (<i>TNNI2</i>), GENE, Vol: 242, Pages: 313-320, ISSN: 0378-1119
- Author Web Link
- Cite
- Citations: 40
Norman DAM, Barton PJR, 2000, Isolation, sequence, and chromosomal localisation of the human IκBR gene (<i>NFKBIL2</i>), ANNALS OF HUMAN GENETICS, Vol: 64, Pages: 15-23, ISSN: 0003-4800
- Author Web Link
- Cite
- Citations: 6
Bhavsar PK, Dellow KA, Yacoub MH, et al., 2000, Identification of <i>cis</i>-acting DNA elements required for expression of the human cardiac troponin I gene promoter, JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, Vol: 32, Pages: 95-108, ISSN: 0022-2828
- Author Web Link
- Cite
- Citations: 47
Burton PBJ, Raff MC, Kerr P, et al., 1999, An intrinsic timer that controls cell-cycle withdrawal in cultured cardiac myocytes, DEVELOPMENTAL BIOLOGY, Vol: 216, Pages: 659-670, ISSN: 0012-1606
- Author Web Link
- Cite
- Citations: 61
Owen VJ, Burton PBJ, Michel MC, et al., 1999, Myocardial dysfunction in donor hearts -: A possible etiology, CIRCULATION, Vol: 99, Pages: 2565-2570, ISSN: 0009-7322
- Author Web Link
- Cite
- Citations: 36
Barton PJR, Cullen ME, Townsend PJ, et al., 1999, Close physical linkage of human troponin genes: Organization, sequence, and expression of the locus encoding cardiac troponin I acid slow skeletal troponin T, GENOMICS, Vol: 57, Pages: 102-109, ISSN: 0888-7543
- Author Web Link
- Cite
- Citations: 26
Burton PBJ, Yacoub MH, Barton PJR, 1999, Cyclin-dependent kinase inhibitor expression in human heart failure - A comparison with fetal development, EUROPEAN HEART JOURNAL, Vol: 20, Pages: 604-611, ISSN: 0195-668X
- Author Web Link
- Cite
- Citations: 42
Norman DAM, Yacoub MH, Barton PJR, 1998, Nuclear factor NF-κB in myocardium:: developmental expression of subunits and activation by interleukin-1β in cardiac myocytes in vitro, CARDIOVASCULAR RESEARCH, Vol: 39, Pages: 434-441, ISSN: 0008-6363
- Author Web Link
- Cite
- Citations: 27
Farza H, Townsend PJ, Carrier L, et al., 1998, Genomic organisation, alternative splicing and polymorphisms of the human cardiac troponin T gene, JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, Vol: 30, Pages: 1247-1253, ISSN: 0022-2828
- Author Web Link
- Cite
- Citations: 37
El Oakley RM, Brand NJ, Burton PBJ, et al., 1998, Efficiency of a high-titer retroviral vector for gene transfer into skeletal myoblasts, JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY, Vol: 115, Pages: 1-8, ISSN: 0022-5223
- Author Web Link
- Cite
- Citations: 10
BARTON PJR, TOWNSEND PJ, BRAND NJ, et al., 1997, Localization of the fast skeletal muscle troponin I gene (TNNI2) to 11p15.5: genes for troponin I and T are organized in pairs, Annals of Human Genetics, Vol: 61, Pages: 519-523, ISSN: 0003-4800
Barton PJR, Townsend PJ, Brand NJ, et al., 1997, Localization of the fast skeletal muscle troponin I gene (TNNI2) to 11pl5.5: genes for troponin I and T are organized in pairs, ANNALS OF HUMAN GENETICS, Vol: 61, Pages: 519-523, ISSN: 0003-4800
- Author Web Link
- Cite
- Citations: 17
Townsend PJ, Yacoub MH, Barton PJR, 1997, Assignment of the human fast skeletal muscle troponin C gene (TNNC2) between D20S721 and GCT10F11 on chromosome 20 by somatic cell hybrid analysis, ANNALS OF HUMAN GENETICS, Vol: 61, Pages: 457-459, ISSN: 0003-4800
- Author Web Link
- Cite
- Citations: 8
TOWNSEND PJ, YACOUB MH, BARTON PJR, 1997, Assignment of the human fast skeletal muscle troponin C gene (TNNC2) between D20S721 and GCT10F11 on chromosome 20 by somatic cell hybrid analysis, Annals of Human Genetics, Vol: 61, Pages: 457-459, ISSN: 0003-4800
TOWNSEND PJ, YACOUB MH, BARTON PJR, 1997, Assignment of the human cardiac/slow skeletal muscle troponin C gene (TNNC1) between D3S3118 and GCT4B10 on the short arm of chromosome 3 by somatic cell hybrid analysis, Annals of Human Genetics, Vol: 61, Pages: 375-377, ISSN: 0003-4800
Townsend PJ, Yacoub MH, Barton PJR, 1997, Assignment of the human cardiac slow skeletal muscle troponin C gene (TNNC1) between D3S3118 and GCT4B10 on the short arm of chromosome 3 by somatic cell hybrid analysis., ANNALS OF HUMAN GENETICS, Vol: 61, Pages: 375-377, ISSN: 0003-4800
- Author Web Link
- Cite
- Citations: 6
Barton PJR, 1996, Gene therapy - The state of play, EUROPEAN HEART JOURNAL, Vol: 17, Pages: 1295-1296, ISSN: 0195-668X
Bhavsar PK, Brand NJ, Yacoub MH, et al., 1996, Isolation and characterization of the human cardiac troponin I gene (TNNI3), GENOMICS, Vol: 35, Pages: 11-23, ISSN: 0888-7543
- Author Web Link
- Cite
- Citations: 68
TOWNSEND PJ, BARTON PJR, YACOUB MH, et al., 1995, MOLECULAR-CLONING OF HUMAN CARDIAC TROPONIN-T ISOFORMS - EXPRESSION IN DEVELOPING AND FAILING HEART, JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, Vol: 27, Pages: 2223-2236, ISSN: 0022-2828
- Author Web Link
- Cite
- Citations: 76
Catala F, Wanner R, Barton P, et al., 1995, A skeletal muscle-specific enhancer regulated by factors binding to E and CArG boxes is present in the promoter of the mouse myosin light-chain 1A gene., Mol Cell Biol, Vol: 15, Pages: 4585-4596, ISSN: 0270-7306
The mouse myosin light-chain 1A (MLC1A) gene, expressed in the atria of the adult heart, is one of the first muscle genes to be activated when skeletal as well as cardiac muscles form in the embryo. It is also transcribed in skeletal muscle cell lines at the onset of differentiation. Transient transfection assays of mouse skeletal muscle cell lines with DNA constructs containing MLC1A promoter fragments fused to the chloramphenicol acetyltransferase (CAT) gene show that the first 630 bp of the promoter is sufficient to direct expression of the reporter gene during myotube formation. Two E boxes located at bp -76 and -519 are necessary for this regulation. MyoD and myogenin proteins bind to them as heterodimers with E12 protein and, moreover, transactivate them in cotransfection experiments with the MLC1A promoter in nonmuscle cells. Interestingly, the effect of mutating each E box is less striking in primary cultures than in the C2 or Sol8 muscle cell line. A DNA fragment from bp -36 to -597 confers tissue- and stage-specific activity to the herpes simplex virus thymidine kinase promoter in both orientations, showing that the skeletal muscle-specific regulation of the MLC1A gene is under the control of a muscle-specific enhancer which extends into the proximal promoter region. At bp -89 is a diverged CArG box, CC(A/T)6AG, which binds the serum response factor (SRF) in myotube nuclear extracts, as does the wild-type sequence, CC(A/T)6GG. Both types of CArG box also bind a novel myotube-enriched complex which has contact points with the AT-rich part of the CArG box and adjacent 3' nucleotides. Mutations within the CArG box distinguish between the binding of this complex and binding of SRF; only SRF binding is directly involved in the specific regulation of the MLC1A gene in skeletal muscle cell lines.
CUMMING DVE, SEYMOUR AML, RIX LK, et al., 1995, TROPONIN-I AND T-PROTEIN EXPRESSION IN EXPERIMENTAL CARDIAC-HYPERTROPHY, CARDIOSCIENCE, Vol: 6, Pages: 65-70, ISSN: 1015-5007
- Author Web Link
- Cite
- Citations: 17
This data is extracted from the Web of Science and reproduced under a licence from Thomson Reuters. You may not copy or re-distribute this data in whole or in part without the written consent of the Science business of Thomson Reuters.