Imperial College London
Portrait Picture

ProfessorPhillipBennett

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Clinical Professor
 
 
 
//

Contact

 

+44 (0)20 7594 2176p.bennett

 
 
//

Assistant

 

Miss Kiran Dosanjh +44 (0)20 7594 2176

 
//

Location

 

Hammersmith HospitalHammersmith Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Kim:2020:10.1016/j.ebiom.2020.103145,
author = {Kim, SH and MacIntyre, D and Binkhamis, R and Cook, J and Sykes, L and Bennett, P and Terzidou, V},
doi = {10.1016/j.ebiom.2020.103145},
journal = {EBioMedicine},
title = {Maternal plasma miRNAs as potential biomarkers for detecting risk of small-for-gestational-age births},
url = {http://dx.doi.org/10.1016/j.ebiom.2020.103145},
volume = {62},
year = {2020}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BackgroundSmall-for-gestational-age fetuses (SGA) (birthweight <10th centile) are at high risk for stillbirth or long-term adverse outcomes. Here, we investigate the ability of circulating maternal plasma miRNAs to determine the risk of SGA births.MethodsMaternal plasma samples from 29 women of whom 16 subsequently delivered normally grown babies and 13 delivered SGA (birthweight <5th centile) were selected from a total of 511 women recruited to form a discovery cohort in which expression data for a total of 800 miRNAs was determined using the Nanostring nCounter miRNA assay. Validation by RT-qPCR was performed in an independent cohort.FindingsPartial least-squares discriminant analysis (PLS-DA) of the Nanostring nCounter miRNA assay initially identified seven miRNAs at 12–14+6 weeks gestation, which discriminated between SGA cases and controls. Four of these were technically validated by RT-qPCR. Differential expression of two miRNA markers; hsa-miR-374a-5p (p = 0•0176) and hsa-let-7d-5p (p = 0•0036), were validated in an independent population of 95 women (SGA n = 12, Control n = 83). In the validation cohort, which was enriched for SGA cases, the ROC AUCs were 0•71 for hsa-miR-374a-5p, and 0•74 for hsa-let-7d-5p, and 0•77 for the two combined.InterpretationWhilst larger population-wide studies are required to validate their performance, these findings highlight the potential of circulating miRNAs to act as biomarkers for early prediction of SGA births.
AU  - Kim,SH
AU  - MacIntyre,D
AU  - Binkhamis,R
AU  - Cook,J
AU  - Sykes,L
AU  - Bennett,P
AU  - Terzidou,V
DO  - 10.1016/j.ebiom.2020.103145
PY  - 2020///
SN  - 2352-3964
TI  - Maternal plasma miRNAs as potential biomarkers for detecting risk of small-for-gestational-age births
T2  - EBioMedicine
UR  - http://dx.doi.org/10.1016/j.ebiom.2020.103145
UR  - http://hdl.handle.net/10044/1/84955
VL  - 62
ER  -
Download