Imperial College London


Faculty of MedicineNational Heart & Lung Institute

Senior Research Fellow







223Guy Scadding BuildingRoyal Brompton Campus






BibTex format

author = {Zhang, Q and Cox, M and Liang, Z and Brinkmann, F and Cardenas, PA and Duff, R and Bhavsar, P and Cookson, W and Moffatt, M and Chung, KF},
doi = {10.1371/journal.pone.0152724},
journal = {PLOS One},
title = {Airway microbiota in severe asthma and relationship to asthma severity and phenotypes},
url = {},
volume = {11},
year = {2016}

RIS format (EndNote, RefMan)

AB - Background: The lower airways harbor a community of bacterial species which is altered in asthma. Objectives: We examined whether the lower airway microbiota were related to measures of asthma severityMethods: We prospectively recruited 26 severe asthma, 18 non-severe asthma and 12 healthy subjects. DNA was extracted from induced sputum and PCR amplification of the V3-V5 region of bacterial 16S rRNA gene was performed. Results: We obtained 138,218 high quality sequences which were rarefied at 133 sequences/sample. Twenty OTUs had sequences ≥1% of total. There were marked differences in the distribution of Phyla between groups (P=2.8x10-118). Bacteroidetes and Fusobacteria were reduced in non-severe and severe asthmatic groups. Proteobacteria were more common in non-severe asthmatics compared to controls (OR=2.26; 95% CI=1.94-2.64) and Firmicutes were increased in severe asthmatics compared to controls (OR=2.15; 95%CI=1.89-2.45). Streptococcal OTUs amongst the Firmicutes were associated with recent onset asthma, rhinosinusitis and sputum eosinophilia.Conclusions: Sputum microbiota in severe asthma differs from healthy controls and non-severe asthmatics, and is characterized by the presence of Streptococcus spp with eosinophilia. Whether these organisms are causative for the pathophysiology of asthma remains to be determined.
AU - Zhang,Q
AU - Cox,M
AU - Liang,Z
AU - Brinkmann,F
AU - Cardenas,PA
AU - Duff,R
AU - Bhavsar,P
AU - Cookson,W
AU - Moffatt,M
AU - Chung,KF
DO - 10.1371/journal.pone.0152724
PY - 2016///
SN - 1932-6203
TI - Airway microbiota in severe asthma and relationship to asthma severity and phenotypes
T2 - PLOS One
UR -
UR -
VL - 11
ER -