Imperial College London
Alternate

DrPankajBhavsar

Faculty of Medicine, National Heart & Lung Institute

Senior Research Fellow
 
 
 
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Contact

 

p.bhavsar

 
 
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Location

 

227AGuy Scadding BuildingRoyal Brompton Campus

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Summary

 

Publications

Citation

BibTex format

@article{Zhang:2016:10.1371/journal.pone.0152724,
author = {Zhang, Q and Cox, M and Liang, Z and Brinkmann, F and Cardenas, PA and Duff, R and Bhavsar, P and Cookson, W and Moffatt, M and Chung, KF},
doi = {10.1371/journal.pone.0152724},
journal = {PLOS One},
title = {Airway microbiota in severe asthma and relationship to asthma severity and phenotypes},
url = {http://dx.doi.org/10.1371/journal.pone.0152724},
volume = {11},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Background: The lower airways harbor a community of bacterial species which is altered in asthma. Objectives: We examined whether the lower airway microbiota were related to measures of asthma severityMethods: We prospectively recruited 26 severe asthma, 18 non-severe asthma and 12 healthy subjects. DNA was extracted from induced sputum and PCR amplification of the V3-V5 region of bacterial 16S rRNA gene was performed. Results: We obtained 138,218 high quality sequences which were rarefied at 133 sequences/sample. Twenty OTUs had sequences ≥1% of total. There were marked differences in the distribution of Phyla between groups (P=2.8x10-118). Bacteroidetes and Fusobacteria were reduced in non-severe and severe asthmatic groups. Proteobacteria were more common in non-severe asthmatics compared to controls (OR=2.26; 95% CI=1.94-2.64) and Firmicutes were increased in severe asthmatics compared to controls (OR=2.15; 95%CI=1.89-2.45). Streptococcal OTUs amongst the Firmicutes were associated with recent onset asthma, rhinosinusitis and sputum eosinophilia.Conclusions: Sputum microbiota in severe asthma differs from healthy controls and non-severe asthmatics, and is characterized by the presence of Streptococcus spp with eosinophilia. Whether these organisms are causative for the pathophysiology of asthma remains to be determined.
AU  - Zhang,Q
AU  - Cox,M
AU  - Liang,Z
AU  - Brinkmann,F
AU  - Cardenas,PA
AU  - Duff,R
AU  - Bhavsar,P
AU  - Cookson,W
AU  - Moffatt,M
AU  - Chung,KF
DO  - 10.1371/journal.pone.0152724
PY  - 2016///
SN  - 1932-6203
TI  - Airway microbiota in severe asthma and relationship to asthma severity and phenotypes
T2  - PLOS One
UR  - http://dx.doi.org/10.1371/journal.pone.0152724
UR  - http://hdl.handle.net/10044/1/30430
VL  - 11
ER  -
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