Imperial College London
Alternate

Professor Patrice D. Cani

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Visiting Professor
 
 
 
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Contact

 

p.cani Website CV

 
 
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Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Plovier:2017:10.1038/nm.4236,
author = {Plovier, H and Everard, A and Depommier, C and Van, Hul M and Geurts, L and Ottman, N and Chilloux, J and Duparc, T and Lichtenstein, L and Myridakis, A and Delzenne, NM and Klievink, J and Bhattacharjee, A and van, der Ark KC and Aalvink, S and Martinez, LO and Dumas, ME and Maiter, D and Loumaye, A and Hermans, MP and Thissen, JP and Belzer, C and de, Vos WM and Cani, PD},
doi = {10.1038/nm.4236},
journal = {Nature Medicine},
pages = {107--113},
title = {A purified membrane protein from Akkermansia muciniphila or the pasteurized bacterium improves metabolism in obese and diabetic mice},
url = {http://dx.doi.org/10.1038/nm.4236},
volume = {23},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Obesity  and  type  2  diabetes  are  associated  with  low-grade  inflammation  and  specific  changes in gut microbiota composition1-7. We previously demonstrated that administration of Akkermansia   muciniphila   prevents   the   development   of   obesity   and   associated   complications8. However, its mechanisms of action remain unclear, whilst the sensitivity of A.  muciniphila  to  oxygen  and  the  presence  of  animal-derived  compounds  in  its  growth  medium currently limit the development of translational approaches for human medicine9. Here  we  addressed  these  issues  by  showing  that  A.  muciniphila  retains  its  efficacy  when  grown  on  a  synthetic  medium  compatible  with  human  administration.  Unexpectedly,  we  discovered  that  pasteurization  of  A.  muciniphila  enhanced  its  capacity  to  reduce  fat  mass  development,  insulin  resistance  and  dyslipidemia  in  mice.  These  improvements  were  notably  associated  with  a  modulation  of  the  host  urinary  metabolomics  profile  and  intestinal energy absorption. We demonstrated that Amuc_1100, a specific protein isolated  from the outer membrane of A. muciniphila, interacts with Toll-Like Receptor 2, is stable at temperatures used for pasteurization, improves the gut barrier and partly recapitulates the beneficial  effects  of  the  bacterium.  Finally,  we  showed  that  administration  of  live  or  pasteurized A. muciniphila grown on the synthetic medium is safe in humansThese findings  provide  support  for  the  use  of  different  preparations  of  A.  muciniphila  as  therapeutic  options to target human obesity and associated disorders.
AU  - Plovier,H
AU  - Everard,A
AU  - Depommier,C
AU  - Van,Hul M
AU  - Geurts,L
AU  - Ottman,N
AU  - Chilloux,J
AU  - Duparc,T
AU  - Lichtenstein,L
AU  - Myridakis,A
AU  - Delzenne,NM
AU  - Klievink,J
AU  - Bhattacharjee,A
AU  - van,der Ark KC
AU  - Aalvink,S
AU  - Martinez,LO
AU  - Dumas,ME
AU  - Maiter,D
AU  - Loumaye,A
AU  - Hermans,MP
AU  - Thissen,JP
AU  - Belzer,C
AU  - de,Vos WM
AU  - Cani,PD
DO  - 10.1038/nm.4236
EP  - 113
PY  - 2017///
SN  - 1546-170X
SP  - 107
TI  - A purified membrane protein from Akkermansia muciniphila or the pasteurized bacterium improves metabolism in obese and diabetic mice
T2  - Nature Medicine
UR  - http://dx.doi.org/10.1038/nm.4236
UR  - http://hdl.handle.net/10044/1/42901
VL  - 23
ER  -
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