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@article{Wang:2022:10.1128/mbio.00836-22,
author = {Wang, Y and Ungerleider, N and Hoffman, BA and Kara, M and Farrell, PJ and Flemington, EK and Lee, N and Tibbetts, SA},
doi = {10.1128/mbio.00836-22},
journal = {mBio},
title = {A polymorphism in the Epstein-Barr virus EBER2 noncoding RNA drives in vivo expansion of latently infected B cells},
url = {http://dx.doi.org/10.1128/mbio.00836-22},
volume = {13},
year = {2022}
}

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TY  - JOUR
AB  - The oncogenic gammaherpesviruses, including human Epstein-Barr virus (EBV), human Kaposi’s sarcoma-associated herpesvirus (KSHV), and murine gammaherpesvirus 68 (MHV68, γHV68, MuHV-4), are associated with numerous malignancies, including B cell lymphomas and nasopharyngeal carcinoma. These viruses employ numerous molecular strategies to colonize the host, including the expression of noncoding RNAs (ncRNAs). As the first viral ncRNAs identified, EBV-encoded RNA 1 and 2 (EBER1 and EBER2, respectively) have been investigated extensively for decades; however, their specific in vivo functions remain largely unknown. In work here, we used chimeric MHV68 viruses in an in vivo complementation system to test whether EBV EBER2 contributes to acute and/or chronic phases of infection. Expression of EBER2 derived from EBV strain B95-8 resulted in a significant expansion of latently infected B cells in vivo, which was accompanied by a decrease in virus-infected plasma cells. EBV strains typically carry one of two variants of EBER2, which differ primarily by a 5-nucleotide core polymorphism identified initially in the EBV strain M81. Strikingly, mutation of the 5 nucleotides that define this core polymorphism resulted in the loss of the infected B cell expansion and restored plasma cell infection. This work reveals that the B95-8 variant of EBER2 promotes the expansion of the latently infected B cell pool in vivo and may do so in part through inhibition of terminal differentiation. These findings provide new insight into mechanisms by which viral ncRNAs promote in vivo colonization and further and provide further evidence of the inherent tumorigenic risks associated with gammaherpesvirus manipulation of B cell differentiation.
AU  - Wang,Y
AU  - Ungerleider,N
AU  - Hoffman,BA
AU  - Kara,M
AU  - Farrell,PJ
AU  - Flemington,EK
AU  - Lee,N
AU  - Tibbetts,SA
DO  - 10.1128/mbio.00836-22
PY  - 2022///
SN  - 2150-7511
TI  - A polymorphism in the Epstein-Barr virus EBER2 noncoding RNA drives in vivo expansion of latently infected B cells
T2  - mBio
UR  - http://dx.doi.org/10.1128/mbio.00836-22
UR  - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000806307300002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb
UR  - https://journals.asm.org/doi/10.1128/mbio.00836-22
UR  - http://hdl.handle.net/10044/1/109210
VL  - 13
ER  -

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