Imperial College London
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RESEARCH TECHNICIAN

Faculty of Medicine, National Heart & Lung Institute

Research Technician
 
 
 
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Contact

 

+44 (0)20 7594 7897p.fenwick

 
 
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Location

 

Technician Room 229BGuy Scadding BuildingRoyal Brompton Campus

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Summary

 

Publications

Citation

BibTex format

@article{Iiott:2014:10.1038/ncomms4979,
author = {Iiott, NE and Heward, JA and Roux, B and Tsitsiou, E and Fenwick, PS and Lenzi, L and Goodhead, I and Hertz-Fowler, C and Heger, A and Hall, N and Donnelly, LE and Sims, D and Lindsay, MA},
doi = {10.1038/ncomms4979},
journal = {Nature Communications},
pages = {1--14},
title = {Long non-coding RNAs and enhancer RNAs regulate the lipopolysaccharide-induced inflammatory response in human monocytes},
url = {http://dx.doi.org/10.1038/ncomms4979},
volume = {5},
year = {2014}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Early reports indicate that long non-coding RNAs (lncRNAs) are novel regulators of biological responses. However, their role in the human innate immune response, which provides the initial defence against infection, is largely unexplored. To address this issue, here we characterize the long non-coding RNA transcriptome in primary human monocytes using RNA sequencing. We identify 76 enhancer RNAs (eRNAs), 40 canonical lncRNAs, 65 antisense lncRNAs and 35 regions of bidirectional transcription (RBT) that are differentially expressed in response to bacterial lipopolysaccharide (LPS). Crucially, we demonstrate that knockdown of nuclear-localized, NF-κB-regulated, eRNAs (IL1β-eRNA) and RBT (IL1β-RBT46) surrounding the IL1β locus, attenuates LPS-induced messenger RNA transcription and release of the proinflammatory mediators, IL1β and CXCL8. We predict that lncRNAs can be important regulators of the human innate immune response.
AU  - Iiott,NE
AU  - Heward,JA
AU  - Roux,B
AU  - Tsitsiou,E
AU  - Fenwick,PS
AU  - Lenzi,L
AU  - Goodhead,I
AU  - Hertz-Fowler,C
AU  - Heger,A
AU  - Hall,N
AU  - Donnelly,LE
AU  - Sims,D
AU  - Lindsay,MA
DO  - 10.1038/ncomms4979
EP  - 14
PY  - 2014///
SN  - 2041-1723
SP  - 1
TI  - Long non-coding RNAs and enhancer RNAs regulate the lipopolysaccharide-induced inflammatory response in human monocytes
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/ncomms4979
UR  - https://www.nature.com/articles/ncomms4979
UR  - http://hdl.handle.net/10044/1/40741
VL  - 5
ER  -
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