Imperial College London
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ProfessorPaulFreemont

Faculty of Medicine, Department of Infectious Disease

Chair in Protein Crystallography
 
 
 
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Contact

 

+44 (0)20 7594 5327p.freemont

 
 
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Location

 

259Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Wood:2019:10.1016/j.celrep.2019.08.094,
author = {Wood, TE and Howard, SA and Forster, A and Nolan, LM and Manoli, E and Bullen, NP and Yau, HCL and Hachani, A and Hayward, RD and Whitney, JC and Vollmer, W and Freemont, PS and Filloux, A},
doi = {10.1016/j.celrep.2019.08.094},
journal = {Cell Reports},
pages = {187--201.e7},
title = {The Pseudomonas aeruginosa T6SS delivers a periplasmic toxin that disrupts bacterial cell morphology},
url = {http://dx.doi.org/10.1016/j.celrep.2019.08.094},
volume = {29},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The  type  VI  secretion  system  (T6SS) is crucialin  interbacterial  competition and is avirulence determinant ofmany Gram-negative bacteria. Several T6SS effectorsarecovalently fused to secreted T6SS  structural  components  such  asthe VgrG spike for delivery  into  target  cells.In Pseudomonas aeruginosa, theVgrG2b effector waspreviously proposedto mediatebacterial internalisation into eukaryotic cells. In this work, wefind that the VgrG2b C-terminal domain(VgrG2bC-ter) elicits toxicity in  the  bacterial  periplasm,  counteracted by a  cognate  immunity  protein.We  resolve  thestructure  of VgrG2bC-ter and confirm it is a member ofthezinc-metallopeptidasefamily of enzymes. We show that this effector causesmembrane blebbing atmidcell, whichsuggests a distincttype of T6SS-mediated growthinhibition through interference with cell division, mimicking the impact of β-lactam antibiotics. Ourstudyintroduces a further effector family to the T6SS arsenaland demonstrates that VgrG2b can target both prokaryotic and eukaryotic cells.
AU  - Wood,TE
AU  - Howard,SA
AU  - Forster,A
AU  - Nolan,LM
AU  - Manoli,E
AU  - Bullen,NP
AU  - Yau,HCL
AU  - Hachani,A
AU  - Hayward,RD
AU  - Whitney,JC
AU  - Vollmer,W
AU  - Freemont,PS
AU  - Filloux,A
DO  - 10.1016/j.celrep.2019.08.094
EP  - 201
PY  - 2019///
SN  - 2211-1247
SP  - 187
TI  - The Pseudomonas aeruginosa T6SS delivers a periplasmic toxin that disrupts bacterial cell morphology
T2  - Cell Reports
UR  - http://dx.doi.org/10.1016/j.celrep.2019.08.094
UR  - https://www.sciencedirect.com/science/article/pii/S2211124719311568?via%3Dihub
UR  - http://hdl.handle.net/10044/1/73329
VL  - 29
ER  -
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