Imperial College London
Portrait Picture

ProfessorPaulFreemont

Faculty of Medicine, Department of Infectious Disease

Chair in Protein Crystallography
 
 
 
//

Contact

 

+44 (0)20 7594 5327p.freemont

 
 
//

Location

 

259Sir Alexander Fleming BuildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Hoose:2023:10.1038/s41570-022-00456-9,
author = {Hoose, A and Vellacott, R and Storch, M and Freemont, PS and Ryadnov, MG},
doi = {10.1038/s41570-022-00456-9},
journal = {Nature Reviews Chemistry},
pages = {144--161},
title = {DNA synthesis technologies to close the gene writing gap},
url = {http://dx.doi.org/10.1038/s41570-022-00456-9},
volume = {7},
year = {2023}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Synthetic DNA is of increasing demand across many sectors of research and commercial activities. Engineering biology, therapy, data storage and nanotechnology are set for rapid developments if DNA can be provided at scale and low cost. Stimulated by successes in next generation sequencing and gene editing technologies, DNA synthesis is already a burgeoning industry. However, the synthesis of >200 bp sequences remains unaffordable. To overcome these limitations and start writing DNA as effectively as it is read, alternative technologies have been developed including molecular assembly and cloning methods, template-independent enzymatic synthesis, microarray and rolling circle amplification techniques. Here, we review the progress in developing and commercializing these technologies, which are exemplified by innovations from leading companies. We discuss pros and cons of each technology, the need for oversight and regulatory policies for DNA synthesis as a whole and give an overview of DNA synthesis business models.
AU  - Hoose,A
AU  - Vellacott,R
AU  - Storch,M
AU  - Freemont,PS
AU  - Ryadnov,MG
DO  - 10.1038/s41570-022-00456-9
EP  - 161
PY  - 2023///
SN  - 2397-3358
SP  - 144
TI  - DNA synthesis technologies to close the gene writing gap
T2  - Nature Reviews Chemistry
UR  - http://dx.doi.org/10.1038/s41570-022-00456-9
UR  - https://www.ncbi.nlm.nih.gov/pubmed/36714378
UR  - https://www.nature.com/articles/s41570-022-00456-9
UR  - http://hdl.handle.net/10044/1/102060
VL  - 7
ER  -
Download