Imperial College London

DrPaulHitchen

Faculty of Natural SciencesDepartment of Life Sciences

Glycomics Technologist
 
 
 
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Contact

 

+44 (0)20 7589 5111 ext 55207p.hitchen

 
 
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Location

 

102Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

30 results found

Bouché L, Panico M, Hitchen P, Binet D, Sastre F, Faulds-Pain A, Valiente E, Vinogradov E, Aubry A, Fulton K, Twine S, Logan SM, Wren BW, Dell A, Morris HRet al., 2016, The Type B flagellin of hypervirulent Clostridium difficile is modified with novel sulphonated Peptidylamido-glycans, Journal of Biological Chemistry, Vol: 291, Pages: 25439-25449, ISSN: 1083-351X

Glycosylation of flagellins is a well recognized property of many bacterial species. In this study we describe the structural characterization of novel flagellar glycans from a number of hypervirulent strains of C. difficile. We used mass spectrometry (nano LC- MS and MS/MS analysis) to identify a number of putative glycopeptides which carried a variety of glycoform substitutions each of which was linked through an initial HexNAc residue to Ser or Thr. Detailed analysis of a LLDGSSTEIR glycopeptide released by tryptic digestion, which carried two variant structures, revealed that the glycopeptide contained, in addition to carbohydrate moieties, a novel structural entity. A variety of Electrospray-MS strategies using Q-TOF technology were used to define this entity, including positive- and negative-ion collisionally activated decomposition (CAD) MS/MS which produced unique fragmentation patterns, and high resolution accurate mass measurement to allow derivation of atomic compositions, leading to the suggestion of a Taurine-containing peptidylamido-glycan structure. Finally NMR analysis of flagellin glycopeptides provided complementary information. The glycan portion of the modification was assigned as α-Fuc3N-(1→3)-α-Rha-(1→2)-α-Rha3OMe-(1→3)-β-GlcNAc-(1→)Ser and the novel capping moiety was shown to be comprised of Taurine, Alanine, and Glycine. This is the first report of a novel O-linked sulphonated peptidylamido-glycan moiety decorating a flagellin protein.

Journal article

Leung M-C, Hitchen PG, Ward DG, Messer AE, Marston SBet al., 2013, Z-band Alternatively Spliced PDZ Motif Protein (ZASP) Is the Major <i>O</i>-Linked β-<i>N</i>-Acetylglucosamine-substituted Protein in Human Heart Myofibrils, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 288, Pages: 4891-4898

Journal article

Harrison R, Hitchen PG, Panico M, Morris HR, Mekhaiel D, Pleass RJ, Dell A, Hewitt JE, Haslam SMet al., 2012, Glycoproteomic characterization of recombinant mouse α-dystroglycan, GLYCOBIOLOGY, Vol: 22, Pages: 662-675, ISSN: 0959-6658

Journal article

Graham SA, Antonopoulos A, Hitchen PG, Haslam SM, Dell A, Drickamer K, Taylor MEet al., 2011, Identification of Neutrophil Granule Glycoproteins as Lewis<SUP>x</SUP>-containing Ligands Cleared by the Scavenger Receptor C-type Lectin, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 286, Pages: 24336-24349

Journal article

Jervis AJ, Langdon R, Hitchen P, Lawson AJ, Wood A, Fothergill JL, Morris HR, Dell A, Wren B, Linton Det al., 2010, Characterization of N-Linked Protein Glycosylation in <i>Helicobacter pullorum</i>, JOURNAL OF BACTERIOLOGY, Vol: 192, Pages: 5228-5236, ISSN: 0021-9193

Journal article

Hitchen PG, Twigger K, Valiente E, Langdon RH, Wren BW, Dell Aet al., 2010, Glycoproteomics: a powerful tool for characterizing the diverse glycoforms of bacterial pilins and flagellins, BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 38, Pages: 1307-1313, ISSN: 0300-5127

Journal article

Hitchen P, Brzostek J, Panico M, Butler JA, Morris HR, Dell A, Linton Det al., 2010, Modification of the <i>Campylobacter jejuni</i> flagellin glycan by the product of the Cj1295 homopolymeric-tract-containing gene, MICROBIOLOGY-SGM, Vol: 156, Pages: 1953-1962, ISSN: 1350-0872

Journal article

Peyfoon E, Meyer B, Hitchen PG, Panico M, Morris HR, Haslam SM, Albers S-V, Dell Aet al., 2010, The <i>S</i>-Layer Glycoprotein of the Crenarchaeote <i>Sulfolobus acidocaldarius</i> Is Glycosylated at Multiple Sites with Chitobiose-Linked <i>N</i>-Glycans, ARCHAEA-AN INTERNATIONAL MICROBIOLOGICAL JOURNAL, Vol: 2010, ISSN: 1472-3646

Journal article

North SJ, Hitchen PG, Haslam SM, Dell Aet al., 2009, Mass spectrometry in the analysis of <i>N</i>-linked and <i>O</i>-linked glycans, CURRENT OPINION IN STRUCTURAL BIOLOGY, Vol: 19, Pages: 498-506, ISSN: 0959-440X

Journal article

Powlesland AS, Hitchen PG, Parry S, Graham SA, Marcela Barrio M, Teresa Elola M, Mordoh J, Dell A, Drickamer K, Taylor MEet al., 2009, Targeted glycoproteomic identification of cancer cell glycosylation, GLYCOBIOLOGY, Vol: 19, Pages: 899-909, ISSN: 0959-6658

Journal article

Qazi O, Hitchen P, Tissot B, Panico M, Morris HR, Dell A, Fairweather Net al., 2009, Mass spectrometric analysis of the S-layer proteins from <i>Clostridium difficile</i> demonstrates the absence of glycosylation, JOURNAL OF MASS SPECTROMETRY, Vol: 44, Pages: 368-374, ISSN: 1076-5174

Journal article

Abu-Qarn M, Giordano A, Battaglia F, Trauner A, Hitchen PG, Morris HR, Dell A, Eichler Jet al., 2008, Identification of AglE, a second glycosyltransferase involved in N glycosylation of the <i>Haloferax volcanii</i> S-layer glycoprotein, JOURNAL OF BACTERIOLOGY, Vol: 190, Pages: 3140-3146, ISSN: 0021-9193

Journal article

Hitchen PG, Dell A, 2006, Bacterial glycoproteomics, MICROBIOLOGY-SGM, Vol: 152, Pages: 1575-1580, ISSN: 1350-0872

Journal article

Burguière A, Hitchen PG, Dover LG, Del A, Besra GSet al., 2005, Altered expression profile of mycobacterial surface glycopeptidolipids following treatment with the antifungal azole inhibitors econazole and clotrimazole, MICROBIOLOGY-SGM, Vol: 151, Pages: 2087-2095, ISSN: 1350-0872

Journal article

Wilson JC, Hitchen PG, Frank M, Peak IR, Collins PM, Morris HR, Dell A, Grice IDet al., 2005, Identification of a capsular polysaccharide from <i>Moraxella bovis</i>, CARBOHYDRATE RESEARCH, Vol: 340, Pages: 765-769, ISSN: 0008-6215

Journal article

Linton D, Dorrell N, Hitchen PG, Amber S, Karlyshev AV, Morris HR, Dell A, Valvano MA, Aebi M, Wren BWet al., 2005, Functional analysis of the <i>Campylobacter jejuni</i> N-linked protein glycosylation pathway, MOLECULAR MICROBIOLOGY, Vol: 55, Pages: 1695-1703, ISSN: 0950-382X

Journal article

Feldman MF, Wacker M, Hernandez M, Hitchen PG, Marolda CL, Kowarik M, Morris HR, Dell A, Valvano MA, Aebi Met al., 2005, Engineering N-linked protein glycosylation with diverse O antigen lipopolysaccharide structures in <i>Escherichia coli</i>, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 102, Pages: 3016-3021, ISSN: 0027-8424

Journal article

Hitchen PG, Linton D, Dorrel N, Amber S, Karlyshev AV, Morris HR, Dell A, Valvano MA, Aebi M, Wren BWet al., 2004, Functional analysis of a novel bacterial <i>N</i>-linked protein glycosylation pathway from <i>Campylobacter jejuni</i>., Joint Meeting of the Society-for-Glycobiology/Japanese-Society-for-Carbohydrate-Research, Publisher: OXFORD UNIV PRESS INC, Pages: 1124-1125, ISSN: 0959-6658

Conference paper

Hegge FT, Hitchen PG, Aas FE, Kristiansen H, Lovold C, Egge-Jacobsen W, Panico M, Leong WY, Bull V, Virji M, Morris HR, Dell A, Koomey Met al., 2004, Unique modifications with phosphocholine and phosphoethanolamine define alternate antigenic forms of <i>Neisseria gonorrhoeae</i> type IV pili, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 101, Pages: 10798-10803, ISSN: 0027-8424

Journal article

Prior JL, Prior RG, Hitchen PG, Diaper H, Griffin KF, Morris HR, Dell A, Titball RWet al., 2003, Characterization of the O antigen gene cluster and structural analysis of the O antigen of <i>Francisella tularensis</i> subsp <i>tularensis</i>, JOURNAL OF MEDICAL MICROBIOLOGY, Vol: 52, Pages: 845-851, ISSN: 0022-2615

Journal article

Wacker M, Linton D, Hitchen PG, Nita-Lazar M, Haslam SM, North SJ, Panico M, Morris HR, Dell A, Wren BW, Aebi Met al., 2002, N-linked glycosylation in <i>Campylobacter jejuni</i> and its functional transfer into <i>E-coli</i>, SCIENCE, Vol: 298, Pages: 1790-1793, ISSN: 0036-8075

Journal article

Hitchen PG, Prior JL, Oyston PCF, Panico M, Wren BW, Titball RW, Morris HR, Dell Aet al., 2002, Structural characterization of lipo-oligosaccharide (LOS) from <i>Yersinia pestis</i>:: regulation of LOS structure by the PhoPQ system, MOLECULAR MICROBIOLOGY, Vol: 44, Pages: 1637-1650, ISSN: 0950-382X

Journal article

Kremer L, Gurcha SS, Bifani P, Hitchen PG, Baulard A, Morris HR, Dell A, Brennan PJ, Besra GSet al., 2002, Characterization of a putative α-mannosyltransferase involved in phosphatidylinositol trimannoside biosynthesis in <i>Mycobacterium tuberculosis</i>, BIOCHEMICAL JOURNAL, Vol: 363, Pages: 437-447, ISSN: 0264-6021

Journal article

Prior JL, Parkhill J, Hitchen PG, Mungall KL, Stevens K, Morris HR, Reason AJ, Oyston PCF, Dell A, Wren BW, Titball RWet al., 2001, The failure of different strains of <i>Yersinia pestis</i> to produce lipopolysaccharide O-antigen under different growth conditions is due to mutations in the O-antigen gene cluster, FEMS MICROBIOLOGY LETTERS, Vol: 197, Pages: 229-233, ISSN: 0378-1097

Journal article

Prior JL, Hitchen PG, Williamson ED, Reason AJ, Morris HR, Dell A, Wren BW, Titball RWet al., 2001, Characterization of the lipopolysaccharide of <i>Yersinia pestis</i>, MICROBIAL PATHOGENESIS, Vol: 30, Pages: 49-57, ISSN: 0882-4010

Journal article

Linton D, Gilbert M, Hitchen PG, Dell A, Morris HR, Wakarchuk WW, Gregson NA, Wren BWet al., 2000, Phase variation of a β-1,3 galactosyltransferase involved in generation of the ganglioside GM<sub>1</sub>-like lipo-oligosaccharide of <i>Campylobacter jejuni</i>, MOLECULAR MICROBIOLOGY, Vol: 37, Pages: 501-514, ISSN: 0950-382X

Journal article

Linton D, Karlyshev AV, Hitchen PG, Morris HR, Dell A, Gregson NA, Wren BWet al., 2000, Multiple <i>N</i>-acetyl neuraminic acid synthetase (<i>neuB</i>) genes in <i>Campylobacter jejuni</i>:: identification and characterization of the gene involved in sialylation of lipo-oligosaccharide, MOLECULAR MICROBIOLOGY, Vol: 35, Pages: 1120-1134, ISSN: 0950-382X

Journal article

Simpson DZ, Hitchen PG, Elmhirst EL, Taylor MEet al., 1999, Multiple interactions between pituitary hormones and the mannose receptor, BIOCHEMICAL JOURNAL, Vol: 343, Pages: 403-411, ISSN: 0264-6021

Journal article

Hitchen PG, Mullin NP, Taylor ME, 1998, Orientation of sugars bound to the principal C-type carbohydrate-recognition domain of the macrophage mannose receptor, BIOCHEMICAL JOURNAL, Vol: 333, Pages: 601-608, ISSN: 0264-6021

Journal article

Mullin NP, Hitchen PG, Taylor ME, 1997, Mechanism of Ca2+ and monosaccharide binding to a C-type carbohydrate-recognition domain of the macrophage mannose receptor, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 272, Pages: 5668-5681, ISSN: 0021-9258

Journal article

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