Publications
30 results found
Bouché L, Panico M, Hitchen P, et al., 2016, The Type B flagellin of hypervirulent Clostridium difficile is modified with novel sulphonated Peptidylamido-glycans, Journal of Biological Chemistry, Vol: 291, Pages: 25439-25449, ISSN: 1083-351X
Glycosylation of flagellins is a well recognized property of many bacterial species. In this study we describe the structural characterization of novel flagellar glycans from a number of hypervirulent strains of C. difficile. We used mass spectrometry (nano LC- MS and MS/MS analysis) to identify a number of putative glycopeptides which carried a variety of glycoform substitutions each of which was linked through an initial HexNAc residue to Ser or Thr. Detailed analysis of a LLDGSSTEIR glycopeptide released by tryptic digestion, which carried two variant structures, revealed that the glycopeptide contained, in addition to carbohydrate moieties, a novel structural entity. A variety of Electrospray-MS strategies using Q-TOF technology were used to define this entity, including positive- and negative-ion collisionally activated decomposition (CAD) MS/MS which produced unique fragmentation patterns, and high resolution accurate mass measurement to allow derivation of atomic compositions, leading to the suggestion of a Taurine-containing peptidylamido-glycan structure. Finally NMR analysis of flagellin glycopeptides provided complementary information. The glycan portion of the modification was assigned as α-Fuc3N-(1→3)-α-Rha-(1→2)-α-Rha3OMe-(1→3)-β-GlcNAc-(1→)Ser and the novel capping moiety was shown to be comprised of Taurine, Alanine, and Glycine. This is the first report of a novel O-linked sulphonated peptidylamido-glycan moiety decorating a flagellin protein.
Leung M-C, Hitchen PG, Ward DG, et al., 2013, Z-band Alternatively Spliced PDZ Motif Protein (ZASP) Is the Major O-Linked beta-N-Acetylglucosamine-substituted Protein in Human Heart Myofibrils, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 288, Pages: 4891-4898
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- Citations: 10
Harrison R, Hitchen PG, Panico M, et al., 2012, Glycoproteomic characterization of recombinant mouse alpha-dystroglycan, GLYCOBIOLOGY, Vol: 22, Pages: 662-675, ISSN: 0959-6658
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- Citations: 46
Graham SA, Antonopoulos A, Hitchen PG, et al., 2011, Identification of Neutrophil Granule Glycoproteins as Lewis(x)-containing Ligands Cleared by the Scavenger Receptor C-type Lectin, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 286, Pages: 24336-24349
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- Citations: 30
Jervis AJ, Langdon R, Hitchen P, et al., 2010, Characterization of N-Linked Protein Glycosylation in Helicobacter pullorum, JOURNAL OF BACTERIOLOGY, Vol: 192, Pages: 5228-5236, ISSN: 0021-9193
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- Citations: 52
Hitchen PG, Twigger K, Valiente E, et al., 2010, Glycoproteomics: a powerful tool for characterizing the diverse glycoforms of bacterial pilins and flagellins, BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 38, Pages: 1307-1313, ISSN: 0300-5127
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- Citations: 21
Hitchen P, Brzostek J, Panico M, et al., 2010, Modification of the Campylobacter jejuni flagellin glycan by the product of the Cj1295 homopolymeric-tract-containing gene, MICROBIOLOGY-SGM, Vol: 156, Pages: 1953-1962, ISSN: 1350-0872
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- Citations: 41
Peyfoon E, Meyer B, Hitchen PG, et al., 2010, The S-Layer Glycoprotein of the Crenarchaeote Sulfolobus acidocaldarius Is Glycosylated at Multiple Sites with Chitobiose-Linked N-Glycans, ARCHAEA-AN INTERNATIONAL MICROBIOLOGICAL JOURNAL, Vol: 2010, ISSN: 1472-3646
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- Citations: 61
North SJ, Hitchen PG, Haslam SM, et al., 2009, Mass spectrometry in the analysis of N-linked and O-linked glycans, CURRENT OPINION IN STRUCTURAL BIOLOGY, Vol: 19, Pages: 498-506, ISSN: 0959-440X
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- Citations: 164
Powlesland AS, Hitchen PG, Parry S, et al., 2009, Targeted glycoproteomic identification of cancer cell glycosylation, GLYCOBIOLOGY, Vol: 19, Pages: 899-909, ISSN: 0959-6658
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- Citations: 57
Qazi O, Hitchen P, Tissot B, et al., 2009, Mass spectrometric analysis of the S-layer proteins from Clostridium difficile demonstrates the absence of glycosylation, JOURNAL OF MASS SPECTROMETRY, Vol: 44, Pages: 368-374, ISSN: 1076-5174
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- Citations: 15
Abu-Qarn M, Giordano A, Battaglia F, et al., 2008, Identification of AglE, a second glycosyltransferase involved in N glycosylation of the Haloferax volcanii S-layer glycoprotein, JOURNAL OF BACTERIOLOGY, Vol: 190, Pages: 3140-3146, ISSN: 0021-9193
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- Citations: 56
Hitchen PG, Dell A, 2006, Bacterial glycoproteomics, MICROBIOLOGY-SGM, Vol: 152, Pages: 1575-1580, ISSN: 1350-0872
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- Citations: 56
Burguiere A, Hitchen PG, Dover LG, et al., 2005, Altered expression profile of mycobacterial surface glycopeptidolipids following treatment with the antifungal azole inhibitors econazole and clotrimazole, MICROBIOLOGY-SGM, Vol: 151, Pages: 2087-2095, ISSN: 1350-0872
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- Citations: 22
Wilson JC, Hitchen PG, Frank M, et al., 2005, Identification of a capsular polysaccharide from Moraxella bovis, CARBOHYDRATE RESEARCH, Vol: 340, Pages: 765-769, ISSN: 0008-6215
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- Citations: 10
Linton D, Dorrell N, Hitchen PG, et al., 2005, Functional analysis of the Campylobacter jejuni N-linked protein glycosylation pathway, MOLECULAR MICROBIOLOGY, Vol: 55, Pages: 1695-1703, ISSN: 0950-382X
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- Citations: 169
Feldman MF, Wacker M, Hernandez M, et al., 2005, Engineering N-linked protein glycosylation with diverse O antigen lipopolysaccharide structures in Escherichia coli, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 102, Pages: 3016-3021, ISSN: 0027-8424
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- Citations: 309
Hitchen PG, Linton D, Dorrel N, et al., 2004, Functional analysis of a novel bacterial N-linked protein glycosylation pathway from Campylobacter jejuni., Joint Meeting of the Society-for-Glycobiology/Japanese-Society-for-Carbohydrate-Research, Publisher: OXFORD UNIV PRESS INC, Pages: 1124-1125, ISSN: 0959-6658
Hegge FT, Hitchen PG, Aas FE, et al., 2004, Unique modifications with phosphocholine and phosphoethanolamine define alternate antigenic forms of Neisseria gonorrhoeae type IV pili, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 101, Pages: 10798-10803, ISSN: 0027-8424
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- Citations: 106
Prior JL, Prior RG, Hitchen PG, et al., 2003, Characterization of the O antigen gene cluster and structural analysis of the O antigen of Francisella tularensis subsp tularensis, JOURNAL OF MEDICAL MICROBIOLOGY, Vol: 52, Pages: 845-851, ISSN: 0022-2615
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- Citations: 60
Wacker M, Linton D, Hitchen PG, et al., 2002, N-linked glycosylation in Campylobacter jejuni and its functional transfer into E-coli, SCIENCE, Vol: 298, Pages: 1790-1793, ISSN: 0036-8075
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- Citations: 584
Hitchen PG, Prior JL, Oyston PCF, et al., 2002, Structural characterization of lipo-oligosaccharide (LOS) from Yersinia pestis: regulation of LOS structure by the PhoPQ system, MOLECULAR MICROBIOLOGY, Vol: 44, Pages: 1637-1650, ISSN: 0950-382X
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- Citations: 74
Kremer L, Gurcha SS, Bifani P, et al., 2002, Characterization of a putative alpha-mannosyltransferase involved in phosphatidylinositol trimannoside biosynthesis in Mycobacterium tuberculosis, BIOCHEMICAL JOURNAL, Vol: 363, Pages: 437-447, ISSN: 0264-6021
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- Citations: 78
Prior JL, Parkhill J, Hitchen PG, et al., 2001, The failure of different strains of Yersinia pestis to produce lipopolysaccharide O-antigen under different growth conditions is due to mutations in the O-antigen gene cluster, FEMS MICROBIOLOGY LETTERS, Vol: 197, Pages: 229-233, ISSN: 0378-1097
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- Citations: 44
Prior JL, Hitchen PG, Williamson ED, et al., 2001, Characterization of the lipopolysaccharide of Yersinia pestis, MICROBIAL PATHOGENESIS, Vol: 30, Pages: 49-57, ISSN: 0882-4010
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- Citations: 47
Linton D, Gilbert M, Hitchen PG, et al., 2000, Phase variation of a beta-1,3 galactosyltransferase involved in generation of the ganglioside GM(1)-like lipo-oligosaccharide of Campylobacter jejuni, MOLECULAR MICROBIOLOGY, Vol: 37, Pages: 501-514, ISSN: 0950-382X
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- Citations: 164
Linton D, Karlyshev AV, Hitchen PG, et al., 2000, Multiple N-acetyl neuraminic acid synthetase (neuB) genes in Campylobacter jejuni: identification and characterization of the gene involved in sialylation of lipo-oligosaccharide, MOLECULAR MICROBIOLOGY, Vol: 35, Pages: 1120-1134, ISSN: 0950-382X
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- Citations: 118
Simpson DZ, Hitchen PG, Elmhirst EL, et al., 1999, Multiple interactions between pituitary hormones and the mannose receptor, BIOCHEMICAL JOURNAL, Vol: 343, Pages: 403-411, ISSN: 0264-6021
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- Citations: 38
Hitchen PG, Mullin NP, Taylor ME, 1998, Orientation of sugars bound to the principal C-type carbohydrate-recognition domain of the macrophage mannose receptor, BIOCHEMICAL JOURNAL, Vol: 333, Pages: 601-608, ISSN: 0264-6021
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- Citations: 23
Mullin NP, Hitchen PG, Taylor ME, 1997, Mechanism of Ca2+ and monosaccharide binding to a C-type carbohydrate-recognition domain of the macrophage mannose receptor, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 272, Pages: 5668-5681, ISSN: 0021-9258
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- Citations: 80
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