Imperial College London
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DrPaoloInglese

Faculty of Medicine, Institute of Clinical Sciences

Research Associate in Data Science and Machine Learning
 
 
 
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Contact

 

p.inglese14 Website

 
 
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Location

 

Robert Steiner MR unitHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@unpublished{Inglese:2021:10.1101/2021.03.29.437482,
author = {Inglese, P and Huang, X and Wu, V and Lewis, M and Takats, Z},
doi = {10.1101/2021.03.29.437482},
publisher = {Cold Spring Harbor Laboratory},
title = {Mass recalibration for desorption electrospray ionization mass spectrometry imaging using endogenous reference ions},
url = {http://dx.doi.org/10.1101/2021.03.29.437482},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - UNPB
AB  - BackgroundMass spectrometry imaging (MSI) data often consist of tens of thousands of mass spectra collected from a sample surface. During the time necessary to perform a single acquisition, it is likely that uncontrollable factors alter the validity of the initial mass calibration of the instrument, resulting in mass errors of magnitude significantly larger than their theoretical values. This phenomenon has a two-fold detrimental effect: a) it reduces the ability to interpret the results based on the observed signals, b) it can affect the quality of the observed signal spatial distributions. ResultsWe present a post-acquisition computational method capable of reducing the observed mass drift by up to 60 ppm in biological samples, exploiting the presence of typical molecules with a known mass-to-charge ratio. The procedure, tested on time-of-flight (TOF) and Orbitrap mass spectrometry analyzers interfaced to a desorption electrospray ionization (DESI) source, improves the molecular annotation quality and the spatial distributions of the detected ions.ConclusionThe presented method represents a robust and accurate tool for performing post-acquisition mass recalibration of DESI-MSI datasets and can help to increase the reliability of the molecular assignment and the data quality.
AU  - Inglese,P
AU  - Huang,X
AU  - Wu,V
AU  - Lewis,M
AU  - Takats,Z
DO  - 10.1101/2021.03.29.437482
PB  - Cold Spring Harbor Laboratory
PY  - 2021///
TI  - Mass recalibration for desorption electrospray ionization mass spectrometry imaging using endogenous reference ions
UR  - http://dx.doi.org/10.1101/2021.03.29.437482
UR  - https://www.biorxiv.org/content/10.1101/2021.03.29.437482v3
UR  - http://hdl.handle.net/10044/1/96368
ER  -
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