Publications
160 results found
Shahzeidi S, Sarnstrand B, Jeffery PK, et al., 1991, Oral N-acetylcysteine reduces bleomycin-induced collagen deposition in the lungs of mice., Eur Respir J, Vol: 4, Pages: 845-852, ISSN: 0903-1936
N-acetylcysteine (NAC) has been employed in the treatment of acute lung injury but its therapeutic value is as yet unproven. In the present study we examined the effect of both L- and D-forms of NAC as inhibitors of bleomycin-induced fibrosis in mice. We hypothesized that, because of the D-form is not metabolized, it may be more effective than the L-form in ameliorating lung injury and fibrosis. Both drugs were given daily in the drinking water at a dose of approximately 400 mg.kg-1 body weight commencing one week prior to a single intratracheal instillation of bleomycin at a dose of 6 mg.kg-1 body weight. Lung injury was assessed 35 days later by measuring total lung collagen content, lung wet weight and examination of tissues by light and electron microscopy. Total collagen content and lung wet weight of animals receiving bleomycin together with L-NAC were 2.90 +/- 0.03 mg and 0.23 +/- 0.01 g, respectively. The values for collagen content, but not wet weight, were significantly less (p less than 0.05) than those given for bleomycin alone (3.90 +/- 0.02 mg and 0.260 +/- 0.005 g, respectively), but greater than (p less than 0.05) controls (2.10 +/- 0.01 mg and 0.160 +/- 0.002 g, respectively). Values for collagen content and wet weight of animals given bleomycin together with D-NAC (3.10 +/- 0.02 mg and 0.21 +/- 0.01 mg, respectively) were both significantly greater than values for control animals but lower than animals given bleomycin alone.(ABSTRACT TRUNCATED AT 250 WORDS)
HAMID Q, AZZAWI M, YING S, et al., 1991, EXPRESSION OF MESSENGER-RNA FOR INTERLEUKIN-5 IN MUCOSAL BRONCHIAL BIOPSIES FROM ASTHMA, JOURNAL OF CLINICAL INVESTIGATION, Vol: 87, Pages: 1541-1546, ISSN: 0021-9738
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- Citations: 786
JEFFERY PK, 1991, MORPHOLOGY OF THE AIRWAY WALL IN ASTHMA AND IN CHRONIC OBSTRUCTIVE PULMONARY-DISEASE, Publisher: AMER THORACIC SOC, Pages: 1152-1158, ISSN: 0003-0805
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- Citations: 119
Read RC, Wilson R, Rutman A, et al., 1991, Interaction of nontypable Haemophilus influenzae with human respiratory mucosa in vitro., J Infect Dis, Vol: 163, Pages: 549-558, ISSN: 0022-1899
One laboratory strain (SH9) (n = 12) and five clinical isolates of unencapsulated Haemophilus influenzae replicated from 10(4) to 10(8) cfu/ml over 24 h in an organ culture of human respiratory mucosa in which only the intact mucosal surface is exposed. By transmission electron microscopy (TEM), bacteria were not seen in association with normal respiratory epithelium, even after incubation for 24 h. Histology and TEM morphometry demonstrated patchy and occasionally confluent damage to epithelia at this time, with bacteria associated only with cells that were structurally damaged. Scanning electron microscopy revealed an increased quantity of mucus in infected preparations; H. influenzae were associated with mucus by 14 h of incubation and with damaged epithelial cells by 24 h. Fimbriation of H. influenzae increased buccal cell adherence but did not facilitate association with normal respiratory epithelium and failed to increase epithelial damage or association with damaged cells. Epithelial damage may be prerequisite for association of H. influenzae with respiratory epithelium in vitro.
READ RC, WILSON R, RUTMAN A, et al., 1991, INTERACTION OF NONTYPABLE HAEMOPHILUS-INFLUENZAE WITH HUMAN RESPIRATORY MUCOSA INVITRO, JOURNAL OF INFECTIOUS DISEASES, Vol: 163, Pages: 549-558, ISSN: 0022-1899
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- Citations: 133
HAMID Q, AZZAWI M, YING S, et al., 1991, INTERLEUKIN-5 MESSENGER-RNA IN MUCOSAL BRONCHIAL BIOPSIES FROM ASTHMATIC SUBJECTS, INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, Vol: 94, Pages: 169-170, ISSN: 0020-5915
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- Citations: 38
AZZAWI M, BRADLEY B, JEFFERY PK, et al., 1990, IDENTIFICATION OF ACTIVATED LYMPHOCYTES-T AND EOSINOPHILS IN BRONCHIAL BIOPSIES IN STABLE ATOPIC ASTHMA, AMERICAN REVIEW OF RESPIRATORY DISEASE, Vol: 142, Pages: 1407-1413, ISSN: 0003-0805
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- Citations: 804
Sharma RK, Jeffery PK, 1990, Airway beta-adrenoceptor number in cystic fibrosis and asthma., Clin Sci (Lond), Vol: 78, Pages: 409-417, ISSN: 0143-5221
1. Maximal binding capacity (Bmax) and the dissociation constant (KD) for the beta-adrenoceptor antagonist 125I-cyanopindol were estimated in membrane preparations of hilar, lobar/main bronchi (level I) and peripheral lung (level II) of grossly normal lungs resected for bronchial carcinoma. The tissue distribution of 125I-cyanopindol-binding sites was assessed by autoradiography of complementary cryostat sections. The data obtained from the resections for carcinoma and bronchiectasis were used as disease controls for comparison with those obtained from patients with cystic fibrosis and asthma. 2. In carcinoma controls, mean Bmax values (+/- SEM) for airway levels I and II were 89 +/- 4 and 133 +/- 6 fmol/mg of protein, respectively (P less than 0.01). The corresponding KD values at each airway level were similar, i.e. 29 +/- 2 and 33 +/- 1 pmol/l, respectively. Autoradiography revealed that there was dense labelling of bronchial and bronchiolar epithelium and most strikingly of the alveolar wall. 3. Compared with carcinoma controls, mean Bmax values in cystic fibrosis were significantly reduced in membrane preparations of both airway levels I and II (P less than 0.01). Autoradiography showed the reduction was most apparent in alveolar wall and bronchial epithelium. 4. There was a tendency to reduction of Bmax in membrane preparations from patients with bronchiectasis at airway level I, but this failed to reach statistical significance. Autoradiography demonstrated that the density of labelling was significantly reduced in bronchial epithelium and bronchial smooth muscle as compared with carcinoma controls (P less than 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
FREW AJ, MOQBEL R, AZZAWI M, et al., 1990, LYMPHOCYTES-T AND EOSINOPHILS IN ALLERGEN-INDUCED LATE-PHASE ASTHMATIC REACTIONS IN THE GUINEA-PIG, AMERICAN REVIEW OF RESPIRATORY DISEASE, Vol: 141, Pages: 407-413, ISSN: 0003-0805
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- Citations: 114
Jeffery PK, Wardlaw AJ, Nelson FC, et al., 1989, Bronchial biopsies in asthma. An ultrastructural, quantitative study and correlation with hyperreactivity., Am Rev Respir Dis, Vol: 140, Pages: 1745-1753, ISSN: 0003-0805
Little is known of the structural changes in mild asthma. We have studied the light and electron microscopic structure of lobar bronchial biopsies taken at fiberoptic bronchoscopy from 11 atopic asthmatics, four of whom were symptomatic and seven of whom were asymptomatic. The former and three of the latter had bronchial hyperresponsiveness to methacholine (PC20 less than 4 mg/ml). Quantitative comparisons were made with biopsies from ten control subjects with normal airway reactivity; five had hay fever and five were nonatopic healthy volunteers. Complete absence of surface epithelium was found in three cases of symptomatic asthma, and stratified squamous epithelium was present in the fourth. A biopsy from one of the healthy control subjects had also lost its surface epithelium. The degree of epithelial loss in all subjects correlated with the degree of airway reactivity (rs = 0.67, p less than 0.001). The reticular lamina of the epithelial basement membrane showed a trend toward thickening in the seven hyperreactive asthmatics (p less than 0.001: median test). There was a tendency to high numbers of inflammatory cells in the lamina propria, but not in the submucosa, of asthmatics, but the differences between groups did not achieve statistical significance. There were significant alterations (px2 less than 0.001) in the proportions of each type of inflammatory cell found in the lamina propria and submucosa of symptomatic asthmatics: an increase of irregularly shaped lymphocytes contributed most to the observed alteration. Where surface epithelium was present, intraepithelial lymphocytes formed the major proportion of intraepithelial "migratory" cells: 64% in normal control subjects, 78% in subjects with hay fever, and 87% in asymptomatic asthmatics.(ABSTRACT TRUNCATED AT 250 WORDS)
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