Imperial College London
Alternate

Dr. Patrik R. Jones

Faculty of Natural Sciences, Department of Life Sciences

Professor of Metabolic Engineering
 
 
 
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Contact

 

+44 (0)20 7594 5213p.jones

 
 
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Location

 

503Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Sattayawat:2020:10.1073/pnas.1914069117,
author = {Sattayawat, P and Yunus, IS and Jones, PR},
doi = {10.1073/pnas.1914069117},
journal = {Proceedings of the National Academy of Sciences of USA},
pages = {1404--1413},
title = {Bioderivatization as a concept for renewable production of chemicals that are toxic or poorly soluble in the liquid phase},
url = {http://dx.doi.org/10.1073/pnas.1914069117},
volume = {117},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Bio-based production technologies may complement or replace petroleum-based production of chemicals, but they face a number of technical challenges, including product toxicity and/or water insolubility. Plants and microorganisms naturally biosynthesize chemicals that often are converted into derivatives with reduced toxicity or enhanced solubility. Inspired by this principle, we propose a bioderivatization strategy for biotechnological chemicals production, defined as purposeful biochemical derivatization of intended target molecules. As proof of principle, the effects of hydrophobic (e.g., esterification) and hydrophilic (e.g., glycosylation) bioderivatization strategies on the biosynthesis of a relatively toxic and poorly soluble chemical, 1-octanol, were evaluated in Escherichia coli and Synechocystis sp. PCC 6803. The 1-octanol pathway was first optimized to reach product titers at which the host displayed symptoms of toxicity. Solvent overlay used to capture volatile products partially masked product toxicity. Regardless of whether solvent overlay was used, most strains with bioderivatization had a higher molar product titer and product yield, as well as improved cellular growth and glucose consumption, compared with strains without bioderivatization. The positive effect on bioproduction was observed with both the hydrophobic and hydrophilic strategies. Interestingly, in several combinations of genotype/induction strength, bioderivatization had a positive effect on productivity without any apparent effect on growth. We attribute this to enhanced product solubility in the aqueous or solvent fraction of the bioreactor liquid phase (depending on the derivative and medium used), with consequent enhanced product removal. Overall, under most conditions, a benefit of bioproduction was observed, and the bioderivatization strategy could be considered for other similar chemicals as well.
AU  - Sattayawat,P
AU  - Yunus,IS
AU  - Jones,PR
DO  - 10.1073/pnas.1914069117
EP  - 1413
PY  - 2020///
SN  - 0027-8424
SP  - 1404
TI  - Bioderivatization as a concept for renewable production of chemicals that are toxic or poorly soluble in the liquid phase
T2  - Proceedings of the National Academy of Sciences of USA
UR  - http://dx.doi.org/10.1073/pnas.1914069117
UR  - https://www.ncbi.nlm.nih.gov/pubmed/31915296
UR  - http://hdl.handle.net/10044/1/76813
VL  - 117
ER  -
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