Imperial College London
Alternate

Dr. Patrik R. Jones

Faculty of Natural Sciences, Department of Life Sciences

Professor of Metabolic Engineering
 
 
 
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Contact

 

+44 (0)20 7594 5213p.jones

 
 
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Location

 

503Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{De:2018:10.1016/j.ymben.2018.03.001,
author = {De, Porcellinis AJ and Norgaard, H and Brey, LMF and Erstad, SM and Jones, PR and Heazlewood, JL and Sakuragi, Y},
doi = {10.1016/j.ymben.2018.03.001},
journal = {Metabolic Engineering},
pages = {170--183},
title = {Overexpression of bifunctional fructose-1,6-bisphosphatase/sedoheptulose-1,7-bisphosphatase leads to enhanced photosynthesis and global reprogramming of carbon metabolism in Synechococcus sp PCC 7002},
url = {http://dx.doi.org/10.1016/j.ymben.2018.03.001},
volume = {47},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Cyanobacteria fix atmospheric CO2 to biomass and through metabolic engineering can also act as photosynthetic factories for sustainable productions of fuels and chemicals. The Calvin Benson cycle is the primary pathway for CO2 fixation in cyanobacteria, algae and C3 plants. Previous studies have overexpressed the Calvin Benson cycle enzymes, ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) and bifunctional sedoheptulose-1,7-bisphosphatase/fructose-1,6-bisphosphatase (hereafter BiBPase), in both plants and algae, although their impacts on cyanobacteria have not yet been rigorously studied. Here, we show that overexpression of BiBPase and RuBisCO have distinct impacts on carbon metabolism in the cyanobacterium Synechococcus sp. PCC 7002 through physiological, biochemical, and proteomic analyses. The former enhanced growth, cell size, and photosynthetic O2 evolution, and coordinately upregulated enzymes in the Calvin Benson cycle including RuBisCO and fructose-1,6-bisphosphate aldolase. At the same time it downregulated enzymes in respiratory carbon metabolism (glycolysis and the oxidative pentose phosphate pathway) including glucose-6-phosphate dehydrogenase (G6PDH). The content of glycogen was also significantly reduced while the soluble carbohydrate content increased. These results indicate that overexpression of BiBPase leads to global reprogramming of carbon metabolism in Synechococcus sp. PCC 7002, promoting photosynthetic carbon fixation and carbon partitioning towards non-storage carbohydrates. In contrast, whilst overexpression of RuBisCO had no measurable impact on growth and photosynthetic O2 evolution, it led to coordinated increase in the abundance of proteins involved in pyruvate metabolism and fatty acid biosynthesis. Our results underpin that singular genetic modifications in the Calvin Benson cycle can have far broader cellular impact than previously expected. These features could be exploited to more efficiently direct carbons towards desired
AU  - De,Porcellinis AJ
AU  - Norgaard,H
AU  - Brey,LMF
AU  - Erstad,SM
AU  - Jones,PR
AU  - Heazlewood,JL
AU  - Sakuragi,Y
DO  - 10.1016/j.ymben.2018.03.001
EP  - 183
PY  - 2018///
SN  - 1096-7176
SP  - 170
TI  - Overexpression of bifunctional fructose-1,6-bisphosphatase/sedoheptulose-1,7-bisphosphatase leads to enhanced photosynthesis and global reprogramming of carbon metabolism in Synechococcus sp PCC 7002
T2  - Metabolic Engineering
UR  - http://dx.doi.org/10.1016/j.ymben.2018.03.001
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000433423600017&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/60613
VL  - 47
ER  -
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